Evaluation of a new whole‐blood filter that allows preparation of platelet concentrates by platelet‐rich plasma methods

Lozano, Marı́a Luisa; Pérez‐Ceballos, Elena; Rivera, José; Paunovic, Dragica; Candela, M. J.; Vicente, Vicente

doi:10.1046/j.0041-1132.2003.00587.x

Cited by 12 publications

(11 citation statements)

References 21 publications

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“…The use of some LRFs as research resources has been previously described [5, 6], but not the use of the common Terumo LR Express (TLRE) filter that according to the manufacturer (A. Wegehaupt, personal communication, July 1, 2015) has unique physical properties [7]. Here we describe a procedure for recovering leukocytes from TLRE filters and demonstrate that the recovered cells are well-suited for a variety of research applications.…”

Section: Introductionmentioning

confidence: 93%

Recovery and assessment of leukocytes from LR Express filters

et al. 2017

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Leukocytes, or white blood cells, are used for a variety of investigational purposes and they offer advantages over laboratory-adapted cell lines. Leukocytes that are typically discarded by blood banks during the collection of red blood cells, platelets, and plasma can often be obtained for research use. However, the available leukocytes are frequently contained within a blood filtration device, such as the Terumo LR Express (TLRE) filter. In this study, procedures were evaluated for the ability to elute viable leukocytes from TLRE filters. The recovered leukocytes were assessed for composition, growth, and functionality. The large majority (>70%) of leukocytes were eluted with a single reverse-elution procedure and the recovered cells contained representative populations of the major leukocyte subsets. Purified T cells exhibited diverse T cell receptor repertoires, characteristic growth upon mitogen stimulation, and CD4+ T cells were able to support HIV-1 propagation. Purified monocytes were able to be differentiated into phenotypically characteristic populations of macrophages and dendritic cells. Overall, TLRE filters offer an attractive source of primary human cells for research and possibly clinical purposes.

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Section: Introductionmentioning

confidence: 93%

Recovery and assessment of leukocytes from LR Express filters

et al. 2017

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“…To avoid undesirable elimination of platelets, a platelet-sparing LR filter (Imuflex WB-SP; Terumo Corporation, Tokyo, Japan) was developed. This filter has the capability to create blood products that meet or exceed Food and Drug Administration processing (FDA) regulations and has been validated to preserve platelets while effectively leukoreducing whole blood [5][6][7]. FDA regulations, however, do not require in vivo assessment of coagulation thus the impact of the platelet-sparing LR filter on the coagulation profile and function of the platelets within whole blood products is unclear [8].…”

Section: Introductionmentioning

confidence: 99%

In vitro Analysis of the Hemostatic Properties of Whole Blood Products Prepared with a Platelet-Sparing Leukoreduction Filter

Zielinski¹,

Stubbs²,

Sf³

et al. 2018

J Thrombo Cir

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Background: Warm fresh whole blood (WFWB) is an ideal resuscitation fluid for exsanguinating patients but there are myriad logistic and infectious issues associated with its use. Cold whole blood (CWB) may be an acceptable alternative to the reconstituted whole blood (RWB), the current standard of care. A leukoreduction filter has been developed which maintains platelet count while eliminating white blood cells but its effect on platelet function is unknown. We hypothesize that CWB will retain an acceptable functional coagulation profile after filtration and over time.

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“…It would not only contribute to the reduced incidence of cytokine-mediated transfusion reactions but also create the conditions for reduced activation of thrombocytes in vitro, with improved therapeutic efficacy [11].…”

Section: Introductionmentioning

confidence: 99%

The effect of a leukodepletion model on the activation stage of platelets

et al. 2011

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AbstractThe preparation of thrombocyte concentrates with filtration before storage (in-line) makes it possible to avoid the presence of mononuclear cells in the concentrate and proinflammatory cytokines. Therefore, this filtration may result with decreased activation of trombocyte receptors in vitro, which may improve therapeutic efficiancy. Methods. We compared two groups, each with 30 therapeutic doses of concentrated thrombocytes. We prepared the first group using the classic model from the buffy coat and the other with concentrated thrombocyte samples filtrated during sampling, so-called in-line, with the WBC filter Imuflex (Terumo). Mononuclear cells (MNC), thrombocyte, and erythrocyte counts in the units of concentrated thrombocytes were obtained on an automatic cell counter, and we used flow cytometry to measure the expression of surface thrombocyte receptors. The results demonstrated that the trombocytes prepared with pre-storage filtration contained a very low level of mononuclear cells and markedly reduced trombocyte receptors. Conclusion. The number of MNC and expression of surface thrombocyte receptors were markedly lower in the concentrated thrombocyte units prepared with in-line filtration. The thrombocytes prepared in this way contain fewer mononuclear cells, are of higher quality, are more functional, and may produce a better therapeutic effect in vivo.

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Evaluation of a new whole‐blood filter that allows preparation of platelet concentrates by platelet‐rich plasma methods

Cited by 12 publications

References 21 publications

Recovery and assessment of leukocytes from LR Express filters

Recovery and assessment of leukocytes from LR Express filters

In vitro Analysis of the Hemostatic Properties of Whole Blood Products Prepared with a Platelet-Sparing Leukoreduction Filter

The effect of a leukodepletion model on the activation stage of platelets

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