Evaluation of a New Simple and Rapid Enzyme-Linked Immunosorbent Assay Kit for Neopterin Determination

Abstract

A new commercially available enzyme-linked immunosorbent assay (ELISA) kit has been evaluated for the measurement of neopterin concentrations in serum, plasma and urine. This competitive ELISA is technically simple, requires only small sample volume and is rapid to perform. The assay procedure consists of sequential 1.5 h and 10 min room temperature incubation steps. The ELISA is accurate, sensitive, specific, and precise. Linear regression analysis of neopterin concentrations measured with the new ELISA and with an established method yielded a highly significant correlation (r = 0.99). The new assay is applicable to ELISA workstations, thus enabling determination of neopterin in large series of samples. The neopterin ELISA kit has been used in routine laboratory testing of blood donations in a blood bank.

Neopterin: A potential marker in chronic peripheral arterial disease

“…Venous blood samples were obtained from the patients within 12 h of fasting. A Neopterin Elisa kit for quantitative determination (IBL, Hamburg, Germany), as previously described by Westermann et al (26) and Smith et al (27), was used to measure the N concentrations. N values were expressed as nmol/litre (nmol/l).…”

Serum neopterin, tumor necrosis factor-α and soluble tumor necrosis factor receptor II (p75) levels and disease activity in Egyptian female patients with systemic lupus erythematosus

“…A blood sample was drawn from all participants after an overnight fast, centrifuged at 3000 rpm for 20 min after being allowed to clot at room temperature. Sera were separated as soon as possible from the clot of red cells after centrifugation to avoid TNF-a production by blood cells that falsely could increase its values [17] and always kept protected from light during the assay for neopterin [18], then aliquoted and stored at À808C until assayed. ANA and ds-DNA antibodies were positive in all patients.…”

Dissociation of neopterin and 7,8-dihydroneopterin from plasma components before HPLC analysis

“…The level of neopterin in biological fluids is measured either by ELISA [13] or by HPLC with fluorescence detection of the highly fluorescent neopterin. Though the initial purchase price of a HPLC is relatively high compared to ELISA equipment, the cost in consumables for each individual sample is relatively inexpensive making it the method of choice in many research laboratories.…”