Evaluation of a New Etest for Detecting Metallo-β-Lactamases in Routine Clinical Testing

Walsh, Timothy R.; Bolmström, Anne; Qwärnström, Anette; Gales, Ana Cristina

doi:10.1128/jcm.40.8.2755-2759.2002

Cited by 236 publications

(182 citation statements)

References 33 publications

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“…The E test MBL strip, based on a combination of a β lactam substrate and a β lactam/ metallo β lactamase inhibitor is speciÞ cally designed to detect as many clinically relevant MBL as possible. [13] The E test MBL strip (IP-IPE) has the ability to detect metallo β lactamases, both chromosomally and plasmid mediated, in aerobic and anaerobic bacteria. [13] This novel method could be used by clinical laboratories to monitor the emergence of metallo β lactamase in a range of clinically signiÞ cant bacteria and by surveillance network to establish the spread of the enzyme.…”

Section: Discussionmentioning

confidence: 99%

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An evaluation of four different phenotypic techniques for detection of metallo-β-lactamase producing <i> Pseudomonas aeruginosa</i>

Behera

Mathur

Das

et al. 2008

Indian J Med Microbiol

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Purpose: The present study was undertaken to detect metallo-β-lactamase (MBL) in nosocomial isolates of Pseudomonas aeruginosa by four different phenotypic methods. Methods: Ninety-one consecutive P. aeruginosa isolates were subjected to susceptibility testing by disc-diffusion assay and Vitek 2. Imipenem resistance was determined by three different methods (disc-diffusion, Vitek 2 and E test). Screening for MBL production was done by imipenem-EDTA combined disc test, imipenem-EDTA double-disc synergy test, imipenem-EDTA MBL E test and EDTA disc potentiation using four cephalosporins. Results: Of 63 imipenem resistant isolates, MBL screening could be done in 56 isolates, of which 48 were MBL positive by combined disc test and 36 by the double disc synergy test. For conÞ rmation of MBL production, MBL E test was done in 30 isolates. All the 30 isolates were conÞ rmed to be MBL positive by the MBL E test method. EDTA disc potentiation using four cephalosporins was not very useful for MBL detection. Conclusions: Imipenem-EDTA combined disc test and imipenem-EDTA MBL E test are equally effective for MBL detection, but given the cost-constraints, combined disc test can be used as a convenient screening method in the clinical microbiology laboratory.

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Section: Discussionmentioning

confidence: 99%

“…[13] The E-test was done according to manufacturer's instructions. MIC ratio of IP (Imipenem)/IPI (Imipenem-EDTA) of >8 or >3 log 2 dilutions indicates MBL production [13] (Fig. 4).…”

Section: Mbl E-testmentioning

confidence: 99%

An evaluation of four different phenotypic techniques for detection of metallo-β-lactamase producing <i> Pseudomonas aeruginosa</i>

Behera

Mathur

Das

et al. 2008

Indian J Med Microbiol

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“…Essa metodologia é bastante simples e apresenta praticidade, podendo também ser utilizada para rastreamento de amostras produtoras de MßL, porém apresenta custo mais elevado (74) . Para essa metodologia considera-se amostra produtora de MßL aquela que apresentar (68) .…”

Section: Detecçãounclassified

Metalo-beta-lactamases

Mendes¹,

Castanheira²,

Pignatari³

et al. 2006

J. Bras. Patol. Med. Lab.

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“…The second method consisted of an Etest® MBL-detection strip (AB Biodisk, Dalvägen, Solna, Sweden) placed onto the same agar plate, which was considered positive for MBL whenever the minimal inhibitory concentration (MIC) of imipenem plus EDTA decreased at least three dilutions as compared to the imipenem MIC alone [10]. A strain of P. aeruginosa known to produce SPM-1 metallo-beta-lactamase was used as a positive control in all cases.…”

Section: Methodsmentioning

confidence: 99%

Metallo-β-lactamase-producing Pseudomonas aeruginosa in two hospitals from southern Brazil

Wirth

Picoli²,

Cantarelli

et al. 2009

Braz J Infect Dis

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This study determined the prevalence of metallo-β β β β β-lactamase (MBL)-producing Pseudomonas aeruginosa in two hospitals located in the Southern part of Brazil and compare the performance of two different phenotypic tests. Thirty-one non-repetitive Pseudomonas aeruginosa isolates from various clinical samples from patients admitted to two hospitals located in Rio Grande do Sul, Brazil (twenty-three from a hospital in Porto Alegre City and eight isolates from a hospital in Vale dos Sinos Region). All strains suggestive of possessing MBLs by phenotypic methods were included in this study. Phenotypic detection of MBLs was carried out simultaneously by using both the MBL Etest® and disk approximation test using 2-mercaptopropionic acid close to a ceftazidime disk. Strains positive were further confirmed using molecular techniques for bla VIM , bla IMP and bla SPM-1 . The prevalence of MBLs from samples of inpatients from the hospital located in Porto Alegre was 30.4% and that of inpatients from Vale dos Sinos hospital was only 3.1%. Only MBL type SPM-1 was detected in these samples by molecular analysis and all were detected by the Etest® MBL strips. The prevalence of P. aeruginosa that produce MBLs can be markedly different in distinct geographical areas, even among different hospitals in the same area. In our study, the EDTA-based method was the only method able to detect all strains harboring the SPM-1 enzyme.

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Evaluation of a New Etest for Detecting Metallo-β-Lactamases in Routine Clinical Testing

Cited by 236 publications

References 33 publications

An evaluation of four different phenotypic techniques for detection of metallo-β-lactamase producing <i> Pseudomonas aeruginosa</i>

An evaluation of four different phenotypic techniques for detection of metallo-β-lactamase producing <i> Pseudomonas aeruginosa</i>

Metalo-beta-lactamases

Metallo-β-lactamase-producing Pseudomonas aeruginosa in two hospitals from southern Brazil

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