An evaluation of four different phenotypic techniques for detection of metallo-β-lactamase producing <i> Pseudomonas aeruginosa</i>

Abstract

Purpose: The present study was undertaken to detect metallo-β-lactamase (MBL) in nosocomial isolates of Pseudomonas aeruginosa by four different phenotypic methods. Methods: Ninety-one consecutive P. aeruginosa isolates were subjected to susceptibility testing by disc-diffusion assay and Vitek 2. Imipenem resistance was determined by three different methods (disc-diffusion, Vitek 2 and E test). Screening for MBL production was done by imipenem-EDTA combined disc test, imipenem-EDTA double-disc synergy test, imipenem-EDTA MBL E test and EDTA disc potentiation using four cephalosporins. Results: Of 63 imipenem resistant isolates, MBL screening could be done in 56 isolates, of which 48 were MBL positive by combined disc test and 36 by the double disc synergy test. For conÞ rmation of MBL production, MBL E test was done in 30 isolates. All the 30 isolates were conÞ rmed to be MBL positive by the MBL E test method. EDTA disc potentiation using four cephalosporins was not very useful for MBL detection. Conclusions: Imipenem-EDTA combined disc test and imipenem-EDTA MBL E test are equally effective for MBL detection, but given the cost-constraints, combined disc test can be used as a convenient screening method in the clinical microbiology laboratory.

Growing Menace of Antibacterial Resistance in Clinical Isolates ofPseudomonas aeruginosain Nepal: An Insight of Beta-Lactamase Production

“…All the beta-lactam antibiotics tested in this study showed resistance rate up to 100%, much higher than the study conducted in Nepal by Mishra et al in 2012 [19], Sherchan et al in 2012 [20], and Khan et al in 2014 [16]. Similarly, the findings of our study corroborate the observations of other authors such as Rafiee et al (Iran) [21], Basak et al [4], and Behera et al from India [22]. …”

Prevalence of Extended Spectrum Betalactamase (ESBL) and Metallobetalactamase (MBL) ProducingPseudomonas aeruginosaandAcinetobacter baumanniiIsolated from Various Clinical Samples

“…The results are similar to Upadhyay et al [16] who had reported MBL production in P. aeruginosa as 20.8%. Sadhna et al [10], Madhu et al [17], and Behera et al [18] have reported MBL production in P. aeruginosa as 41.0%, 61.5%, and 69.5%, respectively, which is higher as compared to this study. Aggarwal et al [19] had reported MBL production to be 11.4% which is lesser as compared to this study.…”

Prevalence of metallo-β-lactamase producing Pseudomonas aeruginosa and Acinetobacter species in intensive care areas in a tertiary care hospital

“…[5] Over the last decade, most of the studies were on different methods of MBL detection in P. aeruginosa and Acinetobacter species. [3456] Though MIC detection is gold standard, DDST and MDST are comparable with the former and at the same time are simple, reliable, less cumbersome and cheap, as per previous reports. [3–5] Lee et al , have reported 100% sensitivity and specificity of MDST.…”