Evaluation of a new enzyme immunoassay (TESTPACK rotavirus) for the detection of rotavirus in fecal specimens

Dennehy, Penelope H.; Gauntlett, D. R.

doi:10.1016/0732-8893(88)90004-1

Cited by 21 publications

(29 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…21,34,43,44 Agreement beyond chance the tests between LA and PAGE showed regular/good agreement, demonstrating low sensitivity for latex agglutination test, as shown in other studies. 20,[45][46][47] Regarding specificity, some authors report that, unlike our findings, they have found values above 90%, where the specificity obtained in this study was 81.9%. 42,48 In general, the results obtained by PAGE and EIA had strong agreement, unlike the findings for PAGE and LA.…”

Section: Discussioncontrasting

confidence: 53%

Rotavirus infection in a tertiary hospital: laboratory diagnosis and impact of immunization on pediatric hospitalization

Pereira¹,

Raboni²,

Nogueira³

et al. 2011

Braz J Infect Dis

View full text Add to dashboard Cite

Background and objectives: Rotavirus (RV) is the main etiological agent of diarrhea in childhood; its laboratory diagnosis is crucial to guide the clinical management and prevention of its spread. RV immunization was introduced in Brazilian 6-month-old children in 2006. The present study was aimed to evaluate three methodologies used for human RV detection in stool samples obtained from patients hospitalized due to gastroenteritis in a teaching hospital and report the impact of RV immunization in hospitalization by diarrhea. Methods: 293 stool samples collected in the 2001-2008 period were analyzed by enzyme immunoassay (EIA), latex agglutination (LA) and polyacrylamide gel electrophoresis (PAGE). Results: Rotavirus was detected in 34.8% of samples by LA assay, 28.3% of samples by EIA assay and in 25.6% of samples by PAGE assay. Considering the PAGE method as gold standard, the sensitivity, specificity and accuracy of EIA were 94.6%, 94.4% and 94.5%, and to LA were 82.6%, 81.6% and 81.9%, respectively. Conclusions: These results indicate that antigen detection by EIA is a rapid, sensitive and specific method, and could be used in large-scale applications for screening stool samples suspected of RV infection. This study showed decreased incidence of RV infection in hospitalized children prior to the implementation of the national immunization program against RV.

show abstract

Section: Discussioncontrasting

confidence: 53%

Rotavirus infection in a tertiary hospital: laboratory diagnosis and impact of immunization on pediatric hospitalization

Pereira¹,

Raboni²,

Nogueira³

et al. 2011

Braz J Infect Dis

View full text Add to dashboard Cite

show abstract

“…ICG test was reported as a sensitive, specific and relatively simple test (Dennehy et al, 1999 (Wilhelmi et al, 2001). …”

Section: Resultsmentioning

confidence: 99%

A Comparative Analysis of Enzyme-Linked Immunosorbent Assay and Rapid Card Test for Diagnosis of Rotavirus Antigen in Acute Diarrhea Below Five Years Children

Agarwal¹,

Garg²,

chand³

et al. 2016

Int.J.Curr.Microbiol.App.Sci

View full text Add to dashboard Cite

“…IC assay is rapid, technically very simple, and showed results comparable to those achieved with EIA [87,88]. Given that these tests have a high sensitivity and specificity (90%-95%), they are widely used in clinical laboratory practice.…”

Section: Antigen Detectionmentioning

confidence: 98%

“…Enzyme immunoassay has been proven to be very sensitive and specific for the detection of group A and C rotaviruses in fecal specimens, especially if monoclonal antibodies are used [73,74]. Immunoassay techniques are also available for the detection of astroviruses, due to the development of monoclonal antibodies against these viruses [75][76][77][78].…”

Section: Antigen Detectionmentioning

confidence: 99%

Diagnosis of viral gastroenteritis: limits and potential of currently available procedures

Sidoti

Rittá

Costa

et al. 2015

J Infect Dev Ctries

View full text Add to dashboard Cite

The diagnostic approaches to viral gastroenteritis have evolved substantially over the past decades because of the advances in detection methods, the emergence of new pathogens, and the increase in diarrhea hospitalizations attributed to viruses, especially in young children in non-industrialized countries. Overall, these factors have lead to a relevant improvement of types and operating characteristics of diagnostic methods (including sensitivity and specificity), as well as turnaround time. In this review, clinical and laboratory approaches to the diagnosis of viruses causing gastroenteritis are presented; in particular, specimen collection and detection methods are reviewed and discussed, taking into account performance and limitations.

show abstract

Evaluation of a new enzyme immunoassay (TESTPACK rotavirus) for the detection of rotavirus in fecal specimens

Cited by 21 publications

References 4 publications

Rotavirus infection in a tertiary hospital: laboratory diagnosis and impact of immunization on pediatric hospitalization

Rotavirus infection in a tertiary hospital: laboratory diagnosis and impact of immunization on pediatric hospitalization

A Comparative Analysis of Enzyme-Linked Immunosorbent Assay and Rapid Card Test for Diagnosis of Rotavirus Antigen in Acute Diarrhea Below Five Years Children

Diagnosis of viral gastroenteritis: limits and potential of currently available procedures

Contact Info

Product

Resources

About