Evaluation of a Native and Introduced Isolate of<i>Pochonia chlamydosporia</i>against<i>Meloidogyne javanica</i>

Sorribas, Francisco Javier; Ornat, C.; Galeano, M.; Verdejo-Lucas, Soledad

doi:10.1080/09583150310001606282

Cited by 28 publications

(15 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To determine fungal population, 1 g of each culture was then washed through 250-and 38-lm sieves to remove sand and bran, and fungal propagules were collected on a 5-lm sieve. The concentration of dictyo-chlamydospores in the inoculum was estimated in diluted samples using a haemacytometer (De Leij and Kerry, 1991;Sorribas et al, 2003).…”

Section: Fungal Isolates and Inoculum Preparationmentioning

confidence: 99%

Pathogenicity of Pochonia species on eggs of Meloidogyne javanica

Moosavi

Zare

Zamanizadeh

et al. 2010

Journal of Invertebrate Pathology

View full text Add to dashboard Cite

Section: Fungal Isolates and Inoculum Preparationmentioning

confidence: 99%

Pathogenicity of Pochonia species on eggs of Meloidogyne javanica

Moosavi

Zare

Zamanizadeh

et al. 2010

Journal of Invertebrate Pathology

View full text Add to dashboard Cite

“…An extensive literature deals with problems encountered in the management of plant-parasitic nematode by means of biocontrol agents and, due to the complexity of the soil environment, the number of NFs-based successful biocontrol tests almost equals unsuccessful reports (Kerry 2000;Sorribas et al 2003;Ornat and Sorribas 2008;Lopez-Llorca et al 2008). A few study deals with biocontrol agents present in areas from extensive agriculture or semi-arid climates, i.e.…”

Section: Discussionmentioning

confidence: 99%

Detection and biocontrol potential of Verticillium leptobactrum parasitizing Meloidogyne spp.

Regaieg

Ciancio²,

Raouani

et al. 2010

World J Microbiol Biotechnol

View full text Add to dashboard Cite

Three isolates of Verticillium leptobactrum proceeding from egg masses of root-knot nematodes (RKN) Meloidogyne spp. and soil samples collected in Tunisia were evaluated against second-stage juveniles (J2) and eggs of M. incognita, to determine the fungus biocontrol potential. In vitro tests showed that V. leptobactrum is an efficient nematode parasite. The fungus also colonized egg masses and parasitized hatching J2. In a greenhouse assay with tomato plants parasitized by M. incognita and M. javanica, V. leptobactrum was compared with isolates of Pochonia chlamydosporia and Monacrosporium sp., introducing the propagules into nematode-free or naturally infested soils. The V. leptobactrum isolates were active in RKN biocontrol, improving plants growth with a significant increase of tomato roots length, lower J2 numbers in soil or egg masses, as well as higher egg mortalities. In a second assay with M. javanica, treatments with three V. leptobactrum isolates reduced egg masses on roots as well as the density of J2 and the number of galls. To evaluate the fungus capability to colonize egg masses a nested Real-time polymerase chain reaction (PCR) assay, based on a molecular beacon probe was used to assess its presence. The probe was designed on a V. leptobactrum ITS region, previously sequenced. This method allowed detection of V. leptobactrum from egg masses, allowing quantitative DNA and fungal biomass estimations.

show abstract

“…One limiting factor is their inconsistent performance in the field (e.g., Lay et al 1982;Dickson and Mitchell 1985;Carneiro and Cayrol 1991;Persson and Jansson 1999;Meyer et al 2001;Sorribas et al 2003), due to the virulence loss (Lohmann et al 1989;Zuckerman et al 1989;Wang et al 2003), insufficient quality control in pre-application steps (Jenkins and Grzywacz 2000) and the fact that with the test scale ascending, more artificially uncontrollable and unknown factors changing with time and space will be involved. Due to the scope of this journal, the pre-application steps will be excluded, a range of complex, integrated, intrinsic (microbial) and external (host and environmental) factors, including chemical and physical properties of the soil, weather conditions, species of host plant, variations in rhizosphere colonisation by MCA, and interactions with soil microflora and fauna, will be considered.…”

Section: Commercialised Microbial Nematicidesmentioning

confidence: 97%

Microbial control of plant-parasitic nematodes: a five-party interaction

2006

View full text Add to dashboard Cite

Plant-parasitic nematodes cause significant economic losses to a wide variety of crops. Chemical control is a widely used option for plantparasitic nematode management. However, chemical nematicides are now being reappraised in respect of environmental hazard, high costs, limited availability in many developing countries or their diminished effectiveness following repeated applications. This review presents progress made in the field of microbial antagonists of plant-parasitic nematodes, including nematophagous fungi, endophytic fungi, actinomycetes and bacteria. A wide variety of microorganisms are capable of repelling, inhibiting or killing plant-parasitic nematodes, but the commercialisation of these microorganisms lags far behind their resource investigation. One limiting factor is their inconsistent performance in the field. No matter how well suited a nematode antagonist is to a target nematode in a laboratory test, rational management decision can be made only by analysing the interactions naturally occurring among ''host plant-nematode target-soil-microbial control agent (MCA)-environment''. As we begin to develop a better understanding of the complex interactions, microbial control of nematodes will be more fine-tuned. Multidisciplinary collaboration and integration of biological control with other control methods will also contribute to more successful control practices.

show abstract

Evaluation of a Native and Introduced Isolate ofPochonia chlamydosporiaagainstMeloidogyne javanica

Cited by 28 publications

References 11 publications

Pathogenicity of Pochonia species on eggs of Meloidogyne javanica

Pathogenicity of Pochonia species on eggs of Meloidogyne javanica

Detection and biocontrol potential of Verticillium leptobactrum parasitizing Meloidogyne spp.

Microbial control of plant-parasitic nematodes: a five-party interaction

Contact Info

Product

Resources

About