2019
DOI: 10.1111/myc.13023
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Evaluation of a Histoplasma antigen lateral flow assay for the rapid diagnosis of progressive disseminated histoplasmosis in Colombian patients with AIDS

Abstract: Summary Background Progressive disseminated histoplasmosis (PDH) is an important cause of mortality in persons living with HIV (PLHIV), especially in countries where patients have limited access to antiretroviral therapies and diagnostic testing. Objective A lateral flow assay (LFA) to detect Histoplasma capsulatum antigen in serum developed by MiraVista® was evaluated. Methods We tested 75 serum samples: 24 from PLHIV and culture‐proven PDH and 51 from PLHIV with other fungal and bacterial infections as well … Show more

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Cited by 43 publications
(36 citation statements)
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References 14 publications
(32 reference statements)
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“…al. [16] recently reported the performance of this test in serum of 75 PLWHIV with suspected histoplasmosis, 24 of them with proven-PDH. They found a sensitivity of 96% and specificity of 90%, by visual reading; while, with the use of an automated reader, the sensitivity and specificity were 92% and 94%, respectively.…”
Section: Plos Neglected Tropical Diseasesmentioning
confidence: 99%
See 1 more Smart Citation
“…al. [16] recently reported the performance of this test in serum of 75 PLWHIV with suspected histoplasmosis, 24 of them with proven-PDH. They found a sensitivity of 96% and specificity of 90%, by visual reading; while, with the use of an automated reader, the sensitivity and specificity were 92% and 94%, respectively.…”
Section: Plos Neglected Tropical Diseasesmentioning
confidence: 99%
“…[14] Two of the commercially available assays were recently validated, one of them (clarus Histoplasma GM Enzyme Immunoassay) showed a sensitivity and specificity of 98% and 97%, respectively when tested in urine samples from HIV patients, [15] while the other [MiraVista Histoplasma Urine Antigen LFA] showed a sensitivity and specificity of 92% and 94%, respectively when serum samples from patients with HIV and suspected PDH were analyzed. [16] Among the molecular tests for the diagnosis of histoplasmosis, the Hcp100 (H. capsulatum protein 100 kDa) nested PCR has been widely used by different authors and has shown a sensitivity of up to 100% and a specificity of 92�4%. [17] Another recently described PCR directed to the genetic sequence called 1281−1283 220 SCAR in H. capsulatum [18], was evaluated together with Hcp100 nested PCR and real-time quantitative PCR (qPCR) for the ITS1 region of the rDNA.…”
Section: Introductionmentioning
confidence: 99%
“…Histoplasma antigen serum lateral flow assay developed at MiraVista Diagnostics was evaluated showing a sensitivity of 95.8% (23 of 24) and a specificity of 90.2% (46 of 51). 77 Serum specimens were stored from culture-proven cases enrolled in a prospective study from May 2008 to August 2011 in Medellin, Columbia. The LFA was performed at the Centers for Disease Control in 2019.…”
Section: Antigen Detectionmentioning
confidence: 99%
“…A Colombian group is working on the development of an IGRA-based assay with promising preliminary results, and a huge potential for the diagnosis of subclinical infections regardless of the immune status of the host (Rubio-Carrasquilla et al, 2019); more results are awaited. A lateral flow device (LFD) for the detection of Histoplasma antigen in serum has been developed recently showing an excellent sensitivity, and extensive validation in non-progressive disseminated cases is expected (Cáceres et al, 2019). Molecular test might be part of the diagnostic armamentarium in settings where clinicians and laboratory personnel are not familiar with this pathogen.…”
Section: Future Directions and Conclusionmentioning
confidence: 99%