Evaluation of a hereditary spherocytosis screening algorithm by automated blood count using reticulocytes and erythrocytic parameters on the Sysmex XN‐series

Sottiaux, Jean-Yves; Favresse, Julien; Chevalier, Charles; Châtelain, Bernard; Jacqmin, Hugues; Mullier, François

doi:10.1111/ijlh.13125

Cited by 6 publications

(4 citation statements)

References 14 publications

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“…Erythrocytic parameters has been already used to help the diagnosis of haemoglobinopathy and membranopathy. Automated reticulocyte indices can differentiate hereditary from acquired spherocytosis ( 4 , 6 ). A previous study, combining reticulocyte count, leukocyte count, and pain in the pelvis and spine, demonstrated a good negative predictive value for ACS risk in SCD ( 24 ).…”

Section: Discussionmentioning

confidence: 99%

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Assessment of Reticulocyte and Erythrocyte Parameters From Automated Blood Counts in Vaso-Occlusive Crisis on Sickle Cell Disease

et al. 2022

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Sickle cell disease is a complex genetic disease involving cell adhesion between red blood cells, white blood cells, platelets and endothelial cells, inducing painful vaso-occlusive crisis (VOC). We assessed reticulocyte and erythrocyte parameters in a cohort of confirmed SCD patients, and investigated whether a combination of these routine laboratory biomarkers of haemolysis could be used to predict VOC development. Reticulocyte and erythrocyte parameters were evaluated using the Sysmex XN-9000 analyser. A total of 98 patients with SCD were included, 72 in steady state and 26 in VOC. Among the 72 patients in steady state, 22 developed a VOC in the following year (median: 3 months [2–6]). The following parameters were increased in SCD patients with VOC development compared to SCD patients without VOC development in the following year: reticulocyte count (94.6 109/L [67.8–128] vs. 48.4 109/L [24.9–87.5]), immature reticulocyte count (259 109/L [181–334] vs. 152 109/L [129–208]) reticulocyte/immature reticulocyte fraction (IRF) ratio (6.63 109/(L*%) [4.67–9.56] vs. 4.94 109/(L*%) [3.96–6.61]), and medium fluorescence reticulocytes (MFR) (19.9% [17.4–20.7] vs. 17.1% [15.95–19.75]). The association of a reticulocyte count of >189.4 109/L and an MFR of >19.75% showed a sensitivity of 81.8% and a specificity of 88% to predict VOC development in the following year. Based on our findings, a combination of routine laboratory biomarkers, as reticulocyte count, immature reticulocyte count and fluorescent reticulocyte fraction at steady state, could be used to predict VOC development in SCD.

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Section: Discussionmentioning

confidence: 99%

“…Whole reticulocyte fraction provides information on bone marrow recovery. However, few studies have evaluated these parameters in sickle cell disease and VOC development ( 3 , 4 , 6 ).…”

Section: Introductionmentioning

confidence: 99%

Assessment of Reticulocyte and Erythrocyte Parameters From Automated Blood Counts in Vaso-Occlusive Crisis on Sickle Cell Disease

et al. 2022

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“…Moreover, the adding of IRF% gives a clear differentiation between the two RBC diseases with high reticulocyte count (SCD and HS). Indeed, IRF% is an excellent parameter to screen HS as previously described by Mullier et al on XE‐series 8 and recently validated by Sottiaux et al on XN‐Series 9 . This second step is necessary to reach a good sensitivity and specificity for RBC disease detection, respectively, of 95.2% and 99.9% and that is why we advise to do a blood smear review and, according to RBC morphology and clinical and biological history, to add further additional laboratory investigations (haemoglobin electrophoresis or EMA test, 10 Figure 3).…”

Section: Discussionmentioning

confidence: 88%

Use of Sysmex XN‐10 red blood cell parameters for screening of hereditary red blood cell diseases and iron deficiency anaemia

Nivaggioni

Bouriche

Coito³

et al. 2020

Int J Lab Hematology

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“…Some RBC and reticulocyte indices, or algorithms containing them, have shown value for the diagnostic screening of hereditary spherocytosis 17 or congenital or acquired dyserythropoietic anemias.…”

Section: Diagnosis and Differential Diagnosis Of Anemiamentioning

confidence: 99%

Full‐field hemocytometry. Forty years of progress seen through Clinical and Laboratory Hematology and the International Journal of Laboratory Hematology

D’Onofrio

2021

Int J Lab Hematology

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The extraordinary advances in clinical hematology, biology, and oncology in the last decades would not have been possible without discovering how to identify and count the cells circulating in the blood. For centuries, scientists have used slides, counting chambers (hemocytometers), and diluting and staining solutions for this task. Then, automated hemocytometry began. This science, now linked to the daily routine of laboratory hematology, has completed an overwhelming path over a few decades. Our laboratories today operate with versatile multiparameter systems, ranging from complex single‐channel instruments to bulky continuous flow machines. In terms of clinical information obtained from a simple routine blood test, the full exploitation of their potential depends on the operators' imagination and courage. A comprehensive review of the scientific publications that have accompanied the development of hemocytometry from the 1950s to today would require entire volumes. More than seven hundred contributions that authors worldwide have published in Clinical and Laboratory Haematology until 2007 and then the International Journal of Laboratory Hematology are summarized. Such journals have represented and hopefully will continue to represent the privileged place of welcome for future scientific research in hemocytometry. Improved technologies, attention to quality, new reagents and electronics, information technology, and scientist talent ensure a more profound and deeper knowledge of cell properties: current laboratory devices measure and count even minor immature or pathological cell subpopulations. Full‐field hemocytometry includes the analysis of nonhematic fluids, digital adds to the microscope, and the development of effective point‐of‐care devices.

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Evaluation of a hereditary spherocytosis screening algorithm by automated blood count using reticulocytes and erythrocytic parameters on the Sysmex XN‐series

Cited by 6 publications

References 14 publications

Assessment of Reticulocyte and Erythrocyte Parameters From Automated Blood Counts in Vaso-Occlusive Crisis on Sickle Cell Disease

Assessment of Reticulocyte and Erythrocyte Parameters From Automated Blood Counts in Vaso-Occlusive Crisis on Sickle Cell Disease

Use of Sysmex XN‐10 red blood cell parameters for screening of hereditary red blood cell diseases and iron deficiency anaemia

Full‐field hemocytometry. Forty years of progress seen through Clinical and Laboratory Hematology and the International Journal of Laboratory Hematology

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