Use of Sysmex XN‐10 red blood cell parameters for screening of hereditary red blood cell diseases and iron deficiency anaemia

Nivaggioni, Vanessa; Bouriche, Lakhdar; Coito, Sylvie; Floch, Anne-Sophie Le; Ibrahim‐Kosta, Manal; Léonnet, Caroline; Arnoux, Isabelle; Loosveld, Marie

doi:10.1111/ijlh.13278

Cited by 6 publications

(6 citation statements)

References 12 publications

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“…Erythrocytic parameters has been already used to help the diagnosis of haemoglobinopathy and membranopathy. Automated reticulocyte indices can differentiate hereditary from acquired spherocytosis ( 4 , 6 ). A previous study, combining reticulocyte count, leukocyte count, and pain in the pelvis and spine, demonstrated a good negative predictive value for ACS risk in SCD ( 24 ).…”

Section: Discussionmentioning

confidence: 99%

“…Another study demonstrated, with RBC, MCV and MCHC, the possibility of diagnostic orientation in SCD ( 25 ). Nivaggioni et al, demonstrated the interest of combining MicroR, MCHC and IRF to differentiate hereditary RBC diseases as spherocytosis and SCD ( 4 ). In our study, we observed an association of increased reticulocyte count and increased reticulocyte/IRF during crises.…”

Section: Discussionmentioning

confidence: 99%

“…However, in daily laboratory practice, no analyser can determine properly RBC morphological abnormalities. These criteria are not always relevant hence the interest to combine several RBC and reticulocyte parameters in order to improve the specificity and sensitivity of microscope examination of a blood smear ( 4 ). The introduction of additional parameters in the past years has opened new opportunities for red cell evaluation.…”

Section: Introductionmentioning

confidence: 99%

“…Whole reticulocyte fraction provides information on bone marrow recovery. However, few studies have evaluated these parameters in sickle cell disease and VOC development ( 3 , 4 , 6 ).…”

Section: Introductionmentioning

confidence: 99%

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Assessment of Reticulocyte and Erythrocyte Parameters From Automated Blood Counts in Vaso-Occlusive Crisis on Sickle Cell Disease

et al. 2022

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Sickle cell disease is a complex genetic disease involving cell adhesion between red blood cells, white blood cells, platelets and endothelial cells, inducing painful vaso-occlusive crisis (VOC). We assessed reticulocyte and erythrocyte parameters in a cohort of confirmed SCD patients, and investigated whether a combination of these routine laboratory biomarkers of haemolysis could be used to predict VOC development. Reticulocyte and erythrocyte parameters were evaluated using the Sysmex XN-9000 analyser. A total of 98 patients with SCD were included, 72 in steady state and 26 in VOC. Among the 72 patients in steady state, 22 developed a VOC in the following year (median: 3 months [2–6]). The following parameters were increased in SCD patients with VOC development compared to SCD patients without VOC development in the following year: reticulocyte count (94.6 109/L [67.8–128] vs. 48.4 109/L [24.9–87.5]), immature reticulocyte count (259 109/L [181–334] vs. 152 109/L [129–208]) reticulocyte/immature reticulocyte fraction (IRF) ratio (6.63 109/(L*%) [4.67–9.56] vs. 4.94 109/(L*%) [3.96–6.61]), and medium fluorescence reticulocytes (MFR) (19.9% [17.4–20.7] vs. 17.1% [15.95–19.75]). The association of a reticulocyte count of >189.4 109/L and an MFR of >19.75% showed a sensitivity of 81.8% and a specificity of 88% to predict VOC development in the following year. Based on our findings, a combination of routine laboratory biomarkers, as reticulocyte count, immature reticulocyte count and fluorescent reticulocyte fraction at steady state, could be used to predict VOC development in SCD.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…Whole reticulocyte fraction provides information on bone marrow recovery. However, few studies have evaluated these parameters in sickle cell disease and VOC development ( 3 , 4 , 6 ).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Assessment of Reticulocyte and Erythrocyte Parameters From Automated Blood Counts in Vaso-Occlusive Crisis on Sickle Cell Disease

et al. 2022

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“…Nivaggioni et al analyzed hematological parameters from the Sysmex XN-10 (a different analyzer than the Sysmex XN-1000) using a two-step decision by classification and regression tree (CART) to predict types of anemia, including iron deficiency anemia, heterozygous haemoglobinopathy, sickle cell disease syndrome, hereditary spherocytosis and other, in 8217 patients (15+ years) from a hospital in France [ 15 ]. Here, the CART method found that the percentage of microcytes, RDW, percentage of nucleated red blood cells, MCH, and immature reticulocyte fraction resulted in a correct disorder classification rate of 99.4%.…”

Section: Discussionmentioning

confidence: 99%

Can Automated Hematology Analyzers Predict the Presence of a Genetic Hemoglobinopathy? An Analysis of Hematological Biomarkers in Cambodian Women

et al. 2021

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Genetic hemoglobinopathies are the most common single-gene disorder worldwide. Some automated hematology analyzers have the capability of flagging individuals who may have hematological disorders based on complete blood count (CBC) biomarkers. We aimed to evaluate the accuracy of a hematology analyzer in identifying genetic hemoglobinopathies in Cambodian women and to determine which hematological biomarkers are the best predictors. A CBC was completed using a Sysmex XN-1000 analyzer and hemoglobinopathies were determined with capillary hemoglobin electrophoresis for 808 nonpregnant Cambodian women. Sysmex XN-1000 Interpretive Program (IP) messages, which flag potential hematological disorders, were produced from CBC results. Then, 2 × 2 tables were used to determine sensitivity and specificity of the IP message “Hemoglobin defect” to detect a genetic hemoglobinopathy. Receiver operating characteristic (ROC) analyses assessed the diagnostic ability of six CBC biomarkers to predict a genetic hemoglobinopathy. In total, 74% of women had a hemoglobinopathy (predominantly Hb E and α-thalassemia). “Hb defect” IP message sensitivity and specificity for genetic hemoglobinopathy detection were 10.4% and 98.6%, respectively. Variable selection strategies yielded a two-variable model including mean corpuscular volume (MCV) and red blood cell (RBC) count (AIC = 99.83, AUCROC = 0.98 (95% CI: 0.97, 0.99)) for the prediction of a homozygous EE disorder. Sensitivity and specificity values do not justify the use of Sysmex XN-1000 IP flag messages for identification of genetic hemoglobinopathies in Cambodian women. Development of an algorithm based on MCV and RBC biomarkers may optimize the screening ability of automated hematology analyzers.

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Screening of subclinical functional hemoglobin and red blood cell abnormalities among blood donors of Fayoum University Hospital in Egypt: Are RET-He, and IRF useful screening tools?

Bakr,

Yousief,

Ezzat

et al. 2023

Transfusion and Apheresis Science

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Use of Sysmex XN‐10 red blood cell parameters for screening of hereditary red blood cell diseases and iron deficiency anaemia

Abstract: This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Cited by 6 publications

References 12 publications

Assessment of Reticulocyte and Erythrocyte Parameters From Automated Blood Counts in Vaso-Occlusive Crisis on Sickle Cell Disease

Assessment of Reticulocyte and Erythrocyte Parameters From Automated Blood Counts in Vaso-Occlusive Crisis on Sickle Cell Disease

Can Automated Hematology Analyzers Predict the Presence of a Genetic Hemoglobinopathy? An Analysis of Hematological Biomarkers in Cambodian Women

Screening of subclinical functional hemoglobin and red blood cell abnormalities among blood donors of Fayoum University Hospital in Egypt: Are RET-He, and IRF useful screening tools?

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