Evaluation of a fully automated and completely quantitative allergen-specific IgE and total IgE detection assay

Huang, Huimin; Huang, Zhifeng; Zheng, Peiyan; Wei, Nili; Liang, Xueqing; Li, Wanjia; Sun, Baoqing

doi:10.1039/d1ay01172c

Cited by 1 publication

(1 citation statement)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The normal range of tIgE level was 0.00–100.00 IU/mL. In this study, according to the international classification standard [14], the cut-off value of HDM-sIgE positive reactivity in this study was 0.35 kU/L; 0.35–3.5 kU/L was considered a low-to-moderate HDM-sIgE level and indicated a weakly positive result (abbreviated as HDM-sIgE weak positive ), and ≥3.5 kU/L was considered a highly elevated concentration and indicated a strongly positive result (abbreviated as HDM-sIgE strong positive ).…”

Section: Methodsmentioning

confidence: 85%

Higher House Dust Mite-Specific IgE Levels among Chronic Spontaneous Urticaria Patients May Implicate Higher Basophil Reactivity

Liang,

Zhou,

Jiang

et al. 2023

Int Arch Allergy Immunol

View full text Add to dashboard Cite

Introduction: Allergen-specific IgE (sIgE) sensitization exists in a considerable fraction of chronic spontaneous urticaria (CSU) patients. Basophils have been implicated in the pathogenesis of CSU. This paper aimed to explore the relationship between allergic sensitization and basophil reactivity in CSU and the possible underlying mechanism. Methods: Basophil-enriched leukocytes were isolated from the peripheral blood of 76 CSU patients and 9 healthy controls. Basophil CD63 and FcεRIα (the alpha subunit of the high-affinity IgE receptor) expression in the blood samples with various house dust mite (HDM)-sIgE levels were determined by flow cytometry. Basophil reactivity and SHIP-1 (a molecule related to the IgE/FcεRI signaling pathway) expression were analyzed after stimulation with an HDM allergen or other stimuli. Results: HDM-sIgEstrong positive (≥3.5 kU/L) CSU patients had a significantly higher mean percentage of basophil CD63 and higher baseline levels of FcεRIα expressed by basophils than HDM-sIgEnormal (<0.35 kU/L) CSU patients and healthy controls; the same went for total serum IgE. After stimulation with Dermatophagoides pteronyssinus peptidase 1 (Derp1) alone or together with Derp1-sIgE, the stimulation index of CD63 and levels of FcεRIα expressed by basophils in HDM-sIgEstrong positive CSU patients were significantly higher than those in HDM-sIgEnormal CSU patients and healthy controls. Significantly more SHIP-1 mRNA expression in HDM-sIgEstrong positive CSU patients was induced after the combined stimulation in comparison to other subjects. Conclusion: CSU patients with higher HDM-sIgE levels (≥3.5 kU/L) may have higher CD63 and FcεRIα expression on peripheral blood basophils. Peripheral blood basophils in these CSU patients are more responsive to HDM allergen stimulation. Higher HDM-sIgE levels among CSU patients may implicate higher basophil reactivity.

show abstract

Section: Methodsmentioning

confidence: 85%

Higher House Dust Mite-Specific IgE Levels among Chronic Spontaneous Urticaria Patients May Implicate Higher Basophil Reactivity

Liang,

Zhou,

Jiang

et al. 2023

Int Arch Allergy Immunol

View full text Add to dashboard Cite

show abstract

Evaluation of a fully automated and completely quantitative allergen-specific IgE and total IgE detection assay

Abstract: Based on the current urgent need for an in vitro quantitative detection system for allergens in most hospitals in China, we introduced a novel allergen-specific immunoglobulin E detection system that...

Cited by 1 publication

References 16 publications

Higher House Dust Mite-Specific IgE Levels among Chronic Spontaneous Urticaria Patients May Implicate Higher Basophil Reactivity

Higher House Dust Mite-Specific IgE Levels among Chronic Spontaneous Urticaria Patients May Implicate Higher Basophil Reactivity

Contact Info

Product

Resources

About