Higher House Dust Mite-Specific IgE Levels among Chronic Spontaneous Urticaria Patients May Implicate Higher Basophil Reactivity

Abstract

<b><i>Introduction:</i></b> Allergen-specific IgE (sIgE) sensitization exists in a considerable fraction of chronic spontaneous urticaria (CSU) patients. Basophils have been implicated in the pathogenesis of CSU. This paper aimed to explore the relationship between allergic sensitization and basophil reactivity in CSU and the possible underlying mechanism. <b><i>Methods:</i></b> Basophil-enriched leukocytes were isolated from the peripheral blood of 76 CSU patients and 9 healthy controls. Basophil CD63 and FcεRIα (the alpha subunit of the high-affinity IgE receptor) expression in the blood samples with various house dust mite (HDM)-sIgE levels were determined by flow cytometry. Basophil reactivity and SHIP-1 (a molecule related to the IgE/FcεRI signaling pathway) expression were analyzed after stimulation with an HDM allergen or other stimuli. <b><i>Results:</i></b> HDM-sIgE<sup>strong positive</sup> (≥3.5 kU/L) CSU patients had a significantly higher mean percentage of basophil CD63 and higher baseline levels of FcεRIα expressed by basophils than HDM-sIgE<sup>normal</sup> (<0.35 kU/L) CSU patients and healthy controls; the same went for total serum IgE. After stimulation with <i>Dermatophagoides pteronyssinus</i> peptidase 1 (Derp1) alone or together with Derp1-sIgE, the stimulation index of CD63 and levels of FcεRIα expressed by basophils in HDM-sIgE<sup>strong positive</sup> CSU patients were significantly higher than those in HDM-sIgE<sup>normal</sup> CSU patients and healthy controls. Significantly more SHIP-1 mRNA expression in HDM-sIgE<sup>strong positive</sup> CSU patients was induced after the combined stimulation in comparison to other subjects. <b><i>Conclusion:</i></b> CSU patients with higher HDM-sIgE levels (≥3.5 kU/L) may have higher CD63 and FcεRIα expression on peripheral blood basophils. Peripheral blood basophils in these CSU patients are more responsive to HDM allergen stimulation. Higher HDM-sIgE levels among CSU patients may implicate higher basophil reactivity.

Density Gradient Centrifugation‐Independent Purification of Human Basophils

“…Moreover, blood donation services do not require donors do disclose all underlying health conditions, such as allergies. This could possibly influence experimental results if basophils from atopic donors are unknowingly used for experiments as basophils from atopic donors have been found to possess higher baseline CD63 externalization levels than those of healthy donors (Liang et al, 2023).…”