Evaluation of a filter system to harvest plasma for identification of failure of passive transfer in newborn calves

Oliveira, L. da C. C.; Borchardt, S.; Heuwieser, W.; Rauch, E.; Erhard, Michael; Sutter, F.

doi:10.3168/jds.2018-15006

Cited by 4 publications

(8 citation statements)

References 33 publications

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“…Filtered plasma obtained through a disposable filter been validated as an analytical medium for FPT assessment in dairy calves using refractometry (da Costa Corrêa Oliveira et al, 2019). Before plasma filtration, each lithium heparin sample was swiveled using a roll mixer for blood samples (RS-TR 5; Phoenix Instrument, Garbsen, Germany) for 10 min.…”

Section: Sutter Et Al: Evaluating Failure Of Passive Transfermentioning

confidence: 99%

“…Coagulation proteins such as fibrinogen are present in the plasma TP. These proteins are clotted during serum processing, leading to lower serum TP results (Lumeij and Maclean, 1996;George, 2001;da Costa Corrêa Oliveira et al, 2019;Elsohaby et al, 2019). Furthermore, plasma tubes contain anticoagulants such as lithium heparin, which can falsely increase plasma TP concentrations if the blood tubes are underfilled (Dubin and Hunt, 1978).…”

Section: Test Characteristicsmentioning

confidence: 99%

“…In addition to serum samples, other media such as centrifuged plasma or filtered plasma are appropriate for the assessment of FPT (da Costa Corrêa Oliveira et al, 2019;Elsohaby et al, 2019). The advantage of using filtered plasma to assess FPT is that a large number of blood samples can be collected and analyzed without the need for centrifugation (da Costa Corrêa Oliveira et al, 2019). In this way, regular assessment of FPT could be integrated into the daily work routine of a dairy farm.…”

Section: Introductionmentioning

confidence: 99%

“…Different studies have used either serum or plasma samples to assess FPT, or they have directly compared the 2 media in a small number of samples (MacFarlane et al, 2014;Villarroel et al, 2014). Elsohaby et al (2019) andda Costa Corrêa Oliveira et al (2019) have published the first studies directly comparing serum and plasma samples from the same dairy calves using a larger sample size (n = 217 and 227, respectively).…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of different analytical methods to assess failure of passive transfer in neonatal calves

Sutter

Rauch²,

Erhard³

et al. 2020

Journal of Dairy Science

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The objective of this study was to evaluate different analytical methods of assessing failure of passive transfer (FPT) in neonatal calves. We hypothesized that 3 different media (i.e., centrifuged serum, centrifuged plasma, filtered plasma) and different analytical methods [i.e., ELISA, capillary electrophoresis (CE), Brix refractometer, and handheld optical refractometer] would be highly correlated with the gold standard radial immunodiffusion (RID) and would generate comparable results. Serum and plasma blood samples were collected from Holstein Friesian calves (n = 216) aged 1 to 7 d, from 2 commercial dairy herds in northeast Germany. The RID analysis showed that 59 of 216 calves (27%) had serum IgG concentrations of <10 mg/mL and 157 calves (73%) had serum concentrations of ≥10 mg/mL. The mean IgG concentration (± standard deviation) was 17.1 ± 9.8 mg/mL, and the range was 0.8 to 47.8 mg/mL. In serum, the correlation between RID and CE was r = 0.97, and between RID and ELISA was r = 0.90; CE and ELISA were also highly correlated (r = 0.89). Both refractometry methods were highly correlated with RID using centrifuged serum, centrifuged plasma, or filtered plasma (Brix refractometer: r = 0.84, 0.80, and 0.78, respectively; handheld optical refractometer: r = 0.83, 0.81, and 0.80, respectively). We determined test characteristics (optimum thresholds, sensitivity, specificity, positive predictive value, negative predictive value, and area under the curve) for CE, ELISA, and the handheld optical and digital refractometers using receiver operating characteristic curve analyses with RID as the reference value. Optimal thresholds for assessing FPT using plasma were higher than for serum, regardless of the method of plasma harvesting. The 4 different devices had comparable areas under the curve, irrespective of the medium used. All analytical methods can be used to assess FPT.

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Section: Sutter Et Al: Evaluating Failure Of Passive Transfermentioning

confidence: 99%

Section: Test Characteristicsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Evaluation of different analytical methods to assess failure of passive transfer in neonatal calves

Sutter

Rauch²,

Erhard³

et al. 2020

Journal of Dairy Science

Self Cite

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“…In practice the collected blood is often left to clot, avoiding centrifugation for reason of practicability. Alternatively, a disposable filter system can be used to evaluate blood samples immediately after collection ( da Costa Corrêa Oliveira et al, 2019 ; Sutter et al, 2020 ).…”

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confidence: 99%

Evaluating different methods of serum collection to detect failed transfer of passive immunity in newborn calves via refractometry

et al. 2023

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Graphical Abstract Summary: The objective of this study was to evaluate the necessity of centrifuging blood samples and to compare 4 different types of serum collection to detect failed transfer of passive immunity (FTPI) in dairy calves. All different types of serum collection showed high correlations and good test characteristics compared with the gold standard. In conclusion, all 4 methods are suited to evaluate FTPI.

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Predicting colostrum and calf blood components based on refractometry

Hue

Williams

Petrovski

et al. 2021

Journal of Dairy Research

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Provision of good quality colostrum is essential for the passive immunity and nutrition of newborn calves. In order to better predict the quality of colostrum and the transfer of passive immunity, the relationships between colostrum components and between calf serum components were examined in this study. Samples of bulk tank milk, colostrum pooled from several cows 0–4 d postpartum, and colostrum collected from individual cows twice daily for 3 d post-partum were compared. With the exception of fat percentage, there were strong correlations between the levels of the components in the pooled colostrum and in the individual cow colostrum collected 0–1 d postpartum. The correlations between total solids as measured by Brix refractometry and total protein, immunoglobulin G (IgG), lactose % and protein % in colostrum within 1 d postpartum and pooled colostrum were 0.92, 0.90, −0.88 and 0.98, respectively. These high correlations enabled these colostrum components to be accurately predicted from Brix % and therefore, the volume of colostrum required to feed neonate calves can be optimised based on Brix refractometry to avoid failure of passive immunity transfer. To assess whether the components obtained from colostrum were correlated in calf blood, newborn calves were separated from their dams before suckling and blood sampled before feeding (day 0), and on days 1 and 7, after receiving colostrum or milk twice a day. The correlations between glucose, total protein, IgG, and gamma-glutamyl transferase (GGT) levels in the calf blood were lower than the correlations observed between the colostrum components. The highest correlation was between serum protein measured by refractometer and serum IgG within one week postpartum. GGT activity was not a good indicator of serum IgG levels. However, serum protein refractometer measurements predicted serum IgG level with high accuracy, providing an on-farm test to determine that calves have received sufficient passive immunity and colostrum components.

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Evaluation of a filter system to harvest plasma for identification of failure of passive transfer in newborn calves

Cited by 4 publications

References 33 publications

Evaluation of different analytical methods to assess failure of passive transfer in neonatal calves

Evaluation of different analytical methods to assess failure of passive transfer in neonatal calves

Evaluating different methods of serum collection to detect failed transfer of passive immunity in newborn calves via refractometry

Predicting colostrum and calf blood components based on refractometry

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