Evaluation of a ddPCR Commercial Assay for the Absolute Quantification of the Monkeypox Virus West Africa in Clinical Samples

Pomari, Elena; Mori, Antonio; Accordini, Silvia; Donini, Annalisa; Cordioli, Maddalena; Tacconelli, Evelina; Castilletti, Concetta

doi:10.3390/diagnostics13071349

Cited by 2 publications

(1 citation statement)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To determine the sensitivity of this assay, different copy numbers of MPXV DNA were used as the template. MPXV DNA was diluted to 10 3 copies, 10 2 copies, 10 copies, and 1 copy as absolute quantified by digital PCR 27 (Supporting Information S3: Figure S3A). Results showed that all four sets of primers and probes were able to detect MPXV DNA as low as 1 copy (Figure 1D).…”

Section: Specificity and Sensitivity Of The Raa-lf Systemmentioning

confidence: 99%

A field diagnostic method for rapid and sensitive detection of mpox virus

Zhao,

Xu,

Wang

et al. 2024

Journal of Medical Virology

View full text Add to dashboard Cite

The mpox outbreak has subdued with fewer reported cases at the present in high‐income countries. It is known that mpox virus (MPXV) infection has been epidemic for more than 50 years in African countries. The ancestral MPXV strain has changed into multiple clades, indicating the ongoing evolution of MPXV, which reflects the historical neglect of mpox in Africa, especially after smallpox eradication, and bestows the danger of more severe mpox epidemics in the future. It is thus imperative to continue the development of mpox diagnostics and treatments so we can be prepared in the event of a new mpox epidemic. In this study, we have developed an MPXV detection tool that leverages the recombinase‐aid amplification assay by integrating lateral flow strips (RAA‐LF) and one‐step sample DNA preparation, with visible readout, no need of laboratory instrument, and ready for field deployment. The detection limit reaches 10 copies per reaction. The performance of our RAA‐FL assay in diagnosing mpox clinical samples is on par with that of the quantitative polymerase chain reaction (PCR) assay. Taken together, we have developed a point‐of‐care RAA‐LF method of high accuracy and sensitivity, readily deployable for field detection of MPXV. This diagnostic tool is expected to improve and accelerate field‐ and self‐diagnosis, allow timely isolation and treatment, reduce the spread of MPXV, thus effectively mitigate MPXV outbreak in the future.

show abstract

Section: Specificity and Sensitivity Of The Raa-lf Systemmentioning

confidence: 99%

A field diagnostic method for rapid and sensitive detection of mpox virus

Zhao,

Xu,

Wang

et al. 2024

Journal of Medical Virology

View full text Add to dashboard Cite

show abstract

Review of virological methods for laboratory diagnosis and characterization of monkeypox virus (MPXV): lessons learned from the 2022 Mpox outbreak

Resman Rus,

Zakotnik,

Sagadin

et al. 2024

Acta Dermatovenerologica Alpina Pannonica Et Adriatica

View full text Add to dashboard Cite

Monkeypox virus (MPXV), originally endemic in West Africa (Clade II) and Central Africa (Clade I), has recently emerged worldwide and has reinforced the need for rapid and accurate MPXV diagnostics. This review presents and critically discusses the range of virological methods for laboratory diagnosis and characterization of MPXV as well as related lessons learned and practical experience gained from the 2022 Mpox global outbreak. Real-time PCR is currently considered the diagnostic gold standard and ensures accurate and timely confirmation of suspected Mpox cases based on suspicious skin lesions, and digital PCR improves the precision of MPXV DNA quantification. Whole genome sequencing reveals the diversity within the Clade IIb outbreak and highlights the role of microevolution in the adaptation of the virus to the human host. Continuous genomic surveillance is important for better understanding of human-to-human transmission and prevention of the emergence of variola virus-like strains. Traditional virological methods such as electron microscopy and virus isolation remain essential for comprehensive virus characterization, particularly in the context of vaccine and antiviral drug development. Despite the current challenges, serological tests detecting a range of anti-MPXV antibodies are important adjunct diagnostic and research tools for confirmation of late-presenting or asymptomatic MPXV cases, contact tracing, epidemiological studies, seroepidemiological surveys, and better understanding of the role of IgG and neutralizing antibodies in the immune response to infection and vaccination. A multidisciplinary approach combining advanced molecular techniques with traditional virological methods is important for rapid and reliable diagnosis, surveillance, and control of the outbreak.

show abstract

Evaluation of a ddPCR Commercial Assay for the Absolute Quantification of the Monkeypox Virus West Africa in Clinical Samples

Cited by 2 publications

References 29 publications

A field diagnostic method for rapid and sensitive detection of mpox virus

A field diagnostic method for rapid and sensitive detection of mpox virus

Review of virological methods for laboratory diagnosis and characterization of monkeypox virus (MPXV): lessons learned from the 2022 Mpox outbreak

Contact Info

Product

Resources

About