Evaluation of a cut‐off value for sperm motility after different hours of incubation to select the suitable reproductive technology (IVF or ICSI)

Zollner, U.; Martin, Stefanie; Liebermann, Jürgen; Steck, Theodore L.

doi:10.1034/j.1600-0412.1999.780410.x

Cited by 6 publications

(8 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The results showed a significant decrease in fertilization rate and 2PN rate with > 4h SI time (p < 0.01), these results could be explained by two conclusions proved before, the sperm motility and viability decline rapidly when spermatozoa were incubated at 37℃ for more than four hours [4]. The fertilization rate will decrease with the sperm loss of motion [15]; and high sperm DNA fragmentation has a significant negative association with fertilization rate [16], more than four hour incubation of sperm will increase the DNA fragmentation [8,9,17].…”

Section: Discussionmentioning

confidence: 58%

The influence of preincubation time of prepared sperm before IVF on fertilization, embryo developmental competence and the reproductive outcomes

Liu

Wang

et al. 2023

Ginekol Pol

View full text Add to dashboard Cite

Objectives: It has been provided that if incubation time of prepared sperm can affect sperm motility and DNA fragment, but little is known about the influence of sperm preincubation time (SI) on the sperm's fertilizing ability, subsequent embryonic development and pregnancy outcomes in in vitro fertilization (IVF). The aim of this 2 study was to explore the association of SI with fertilization rate, embryo development and clinical outcomes in IVF, further, to find an optimal preincubation time for prepared sperm before insemination in IVF. Material and methods:This retrospective cohort study included a total of 1453 infertile couples undergoing IVF in our center performed from January 2016 to January 2019. Sperm were preincubated at 37 ℃ 6% CO2 for different times before insemination. Preincubation time associated with fertilization rate (FR), 2PN rate, D3 good quality embryo rate, fresh embryo implantation rate (IR), blastocyst formation rate, cumulative pregnancy rate (CPR), cumulative ongoing pregnancy rate (COPR), cumulative live birth rate (CLBR), newborn health and gender ratio were analyzed by chi-square analysis.Results: FR and 2PN rate of SI more than four hours SI groups (> 4 h SI group) decreased significantly compared with other SI groups (p < 0.01). There were no significant differences of the D3 high quality embryo rate among five SI groups. The blastocyst formation rate of > 4 h SI group was significantly lower than that of 2-3 h SI group (45.5% vs 56.1%, p < 0.05); and 1-2 h SI group also had significant difference with 2-3 h and 3-4 h SI group (48.9% vs 56.1% and 54.6%, p < 0.05). There were a significant decrease of fresh IR and CPR in ≤1 h SI group compared with 1-2 h SI group (19.6% vs. 38.0%, p < 0.05; 62.7% vs 73.7%, p < 0.05); ≤ 1 h SI group also have the lowest CLBR (45.6%), it had statistic differences with 1-2 SI group and 3-4 SI group (45.6% vs 63.2%, p < 0.01; 45.6% vs 61.2%, p < 0.05). Conclusions:The sperm preincubated time at 37℃ 6% CO2 before insemination could influence sperm fertilizing ability, blastocyst formation, embryo implantation and CLBR in IVF cycles. The best time for prepared sperm preincubation at 37℃ is one to four hours before insemination in IVF.

show abstract

Section: Discussionmentioning

confidence: 58%

The influence of preincubation time of prepared sperm before IVF on fertilization, embryo developmental competence and the reproductive outcomes

Liu

Wang

et al. 2023

Ginekol Pol

View full text Add to dashboard Cite

show abstract

“…The gamete population that demonstrated smaller motility dynamics had a lower fertilization value. In previous studies in which the assessment of correlation between sperm motility parameters and oocytes fertilization rate was made without taking into consideration male gametes kinetic subpopulations, the unambiguous results were not obtained [2,3,5,13].…”

Section: Sperm Kinetics=ivfmentioning

confidence: 87%

EFFECT OF SPERM SUBPOPULATION'S KINETICS ON HUMAN FERTILIZATIONIN VITRO

Jȩdrzejczak

Rzepczynska

Taszarek-Hauke

et al. 2005

Archives of Andrology

View full text Add to dashboard Cite

The present study was carried out to investigate the relationship between sperm subpopulation kinetics on in vitro fertilization rate. The ability of human sperm to achieve fertilization oocytes was investigated in relation to particular motility parameters obtained on a computer aided sperm analysis system base. Analysis covers velocity straight linear (VSL), cross beat frequency (CBF), lateral head displacement (LHD) and homogeneity of progressive motility velocity (HPMV) of fresh semen and semen after density gradient selection. Investigation was based on sperm samples from 82 infertile couples undergoing IVF. Two subpopulations were extracted from each sample using the clustering method with respect to VSL parameter: a slow and rapid one. Comparison of obtained results before and after selection shows no significant change of subpopulations percentage. However, this method of selection strongly influences motility parameters of both subpopulations. There was found a positive correlation for VSL, LHD and HPMV and a negative correlation for CBF parameters found in slow fraction of fresh semen and percentage of fertilized oocytes. On the other hand, rapid subpopulation parameters for fresh semen and parameters found for both subpopulations in semen after selection did not correlate with one. This means that information of slow sperm subpopulation kinetics carries important prognostic value of IVF success. Since the current prognosis factors ignore motility parameters of slow sperms, our results show the importance of such an analysis.

show abstract

“…() also found that the concentration of normal motile spermatozoon had an effect on fertilisation and pregnancy rates. Sperm motility characteristics and resistance to the time of incubation have been observed (Zollner et al ., ; Petrella et al ., ). These authors processed semen samples and determined a cut of value of sperm motility to select the suitable reproductive technology (IVF or ICSI).…”

Section: Discussionmentioning

confidence: 99%

Sperm selection based on motility in polyvinylpyrrolidone is associated with successful pregnancy and embryo development

et al. 2012

View full text Add to dashboard Cite

The aim of this study was to investigate whether spermatozoon motility in polyvinylpyrrolidone (PVP) is associated with better embryo development and pregnancy rates in ICSI cycles. A total of 123 primary ICSI treatment cycles were included in this study. Semen samples were tested for motility before ICSI procedure in PVP. Within 3 min, the presence or absence of motility was recorded. Sperm functions were examined by the aniline blue (AB) chromatin condensation test and the hypoosmotic swelling test, and the chromatin stability was evaluated by inducing its decondensation with sodium dodecyl sulphate and ethylenediaminetetraacetic acid (EDTA). Fertilisation and embryo scoring were evaluated. Fifty (64%) of 78 women conceived in the PVP (+) group; and 12 (26%) of 45 women conceived in the PVP (-) group; the pregnancy rate was significantly higher in the PVP (+) group (P = 0.003). Semen parameters were observed to be similar in both groups. The mean number of total embryos obtained in ICSI procedure and transferred grade 1 embryos were significantly higher in PVP (+) group (P = 0.01 and P = 0.003 respectively). The presence of sperm motility in PVP is associated with increased pregnancy rate, higher percentage of good quality embryos, sperm chromatin condensation and decondensation.

show abstract

Evaluation of a cut‐off value for sperm motility after different hours of incubation to select the suitable reproductive technology (IVF or ICSI)

Abstract: The predictive power of sperm motility after 48 h for fertilization outcome provides support in the decision-making process within the assisted reproduction setting. If less than 20% of sperm are motile after 48 h micromanipulatory techniques should be considered.

Cited by 6 publications

References 19 publications

The influence of preincubation time of prepared sperm before IVF on fertilization, embryo developmental competence and the reproductive outcomes

The influence of preincubation time of prepared sperm before IVF on fertilization, embryo developmental competence and the reproductive outcomes

EFFECT OF SPERM SUBPOPULATION'S KINETICS ON HUMAN FERTILIZATIONIN VITRO

Sperm selection based on motility in polyvinylpyrrolidone is associated with successful pregnancy and embryo development

Contact Info

Product

Resources

About