Evaluation of a commercial latex agglutination test for detecting antibodies to cytomegalovirus in organ donors and transplant recipients

Gray, Jim; Alvey, Barbara; Smith, D.J.; Wreghitt, Tim

doi:10.1016/0166-0934(87)90026-7

Cited by 29 publications

(11 citation statements)

References 7 publications

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“…This localization is also in agreement with in vivo animal experiments; perifollicular red pulp cells are infected in acute infections (30] and are demonstrated to be a site for latency in CMV infected animals [26]. As has been shown in several other studies [6,9,11,20], it is important to use a dependable serological assay for screening blood and organ donors to achieve a reduction of transmitted HCMV infection and HCMV disease in seronegative recipients at risk of primary infection. However, there is some debate about seronegative donors being free from previous HCMV infection and the possibility of presence of latent virus [10,28,29].…”

Section: Discussionsupporting

confidence: 87%

“…Spleen tissue from seropositive patients (kidney donors) known to contain latent HCMV in their arterial walls [12], served as a positive control group for all DNA hybridization techniques. For the detection of HCMV antibodies in the serum samples the latex agglutination test (LA), which is used in most laboratories, and the enzyme linked immuno sorbent assay (ELISA), were used [2,11 ]. To confirm the HCMV antibody negative status of the patients the very sensitive immunoblotting technique described by Landini et al [17] was performed.…”

Section: Abstract: Human Cytomegalovirus-latency-hybridization-antibmentioning

confidence: 99%

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Detection of latent human cytomegalovirus in organ tissue and the correlation with serological status

Kraat

Hendrix

Wijnen

et al. 1992

Transplant International

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The presence of human cytomegalovirus (HCMV) genome in spleen tissue was studied by using DNA hybridization techniques in seropositive and seronegative organ donors without clinical or laboratory confirmed HCMV infection. The serum samples of these patients were screened by latex agglutination test (LA) and enzyme linked immuno sorbent assay (ELISA) for the presence of HCMV antibodies, and confirmed by immunoblotting technique (IB ). For the detection of HCMV sequences in spleen tissue dot blot DNA hybridization (DBH) using probes derived from immediate-early and late regions (ES and BH fragment respectively) of the HCMV genome were used. Samples positive in DBH were further tested by in situ DNA hybridization (ISH) using the ES probe. The number of spleen tissue specimens positive for HCMV nucleic acids indicated that HCMV may be present in human beings, even without serological evidence.Human cytomegalovirus (HCMV), a member of the herpesvirus family, is an ubiquitous human viral pathogen. Infection with HCMV is usually asymptomatic in the immunocompetent host, but can result in a dramatic disease in immunosuppressed patients [15]. After primary infection the virus persists in the host as a chronic or latent infection, which can periodically reactivate to an active infection. HCMV is known to be transmitted through blood transfusions [1,24] and transplanted organs [10,33] which can result in a severe HCMV disease, especially in immunosuppressed and seronegative patients [3,22]. Several studies indicate that in HCMV seropositive healthy individuals latent infection can be demonstrated by the presence of HCMV antigens. This is done by immunohistochemical techniques or by the detecting the presence of Offprint requests to: Y. J. Kraat,

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Section: Discussionsupporting

confidence: 87%

Section: Abstract: Human Cytomegalovirus-latency-hybridization-antibmentioning

confidence: 99%

Detection of latent human cytomegalovirus in organ tissue and the correlation with serological status

Kraat

Hendrix

Wijnen

et al. 1992

Transplant International

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“…Deeply frozen serum samples from 20 of 62 patients of the endometrial biopsy group and the very early pregnancy group were available for the latex agglutination test for CMV antibody testing. 3z , 33 The serum samples were taken within 3 days of the biopsy in 18 patients, 5 days before the endometrial biopsy in one patient and 2 months after endometrial biopsy in another patient. The results of the latex agglutination test were compared with the endometrial findings.…”

Section: Methodsmentioning

confidence: 99%

The Presence of Cytomegalovirus Antigens in Karyotyped Abortions

Lijnschoten¹,

Stals

Evers

et al. 1994

American J Rep Immunol

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“…Latex particles coated with CMV antigens have become a popular serologic tool to detect antihodies by agglutination (Adler et al, 1985). This test has been particularly unduly advocated in screening of blood donors (Adler et al, 1985;LaRocco et al, 1986;McHugh et al, 1985;Traswell et al, 1986;Gray et al, 1987). Some authors report that the test compares favorably in sensitivity and specificity to standard tests such as the ELISA and IHA (Adler et al, 1985;Sererat et al, 1986;LaRocco et al, 1986).…”

Section: Latex Agglutination Testmentioning

confidence: 99%

Cytomegalovirus

1991

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All rights reserved 90-14345 CIP No part of this book may be reproduced, stored in a retrieval system, or transmitted in any form or by any means, electronic, mechanical, photocopying, microfilming, recording, or otherwise, without written permission from the Publisher Preface to the First EditionAlthough there are a number of excellent current reviews on one or another aspect of cytomegalovirus, the last comprehensive treatment of this subject was that of Krech et al. (197la). In view of the amazing advances in the virological, epidemiologic, and clinical knowledge of cytomegaloviruses, an up-to-date book is needed. Such a work should cover many areas of expertise and a voluminous technical literature. Each area might have been reviewed and analyzed by workers more expert than myself. However, I have embarked on the entire venture alone in order to attain unity and continuity in this book, characteristics that are not easily achieved in the more popular multiauthored works. I have tried to review the Iiterature and provide a critical summary for each area discussed. To do this, I provide as much of the primary data of the relevant works as needed and not just the qualitative conclusions. Inevitably, the flow of the narrative may be interrupted by dry facts and figures. However, such information is essential to make this a meaningful reference work. But for those not interested in such details, I have provided at what I hope are crucial points critiques and summaries.This book is not an exhaustive review of all the literature. This is probably no Ionger possible or even desirable. By selection, however, one runs the risk of having missed or ignored important papers. I am keenly aware of this, and I wish to apologize for such oversight, if that is possible.I visualize this work as being useful for physicians, scientists, and students interested in the biology of and infection by human cytomegalovirus. I feel that to understand these, the basic virology of this virus and related herpesviruses must be included. Topics that have not advanced a great deal and that are adequately described in available works, such as the anatomic pathology, have not been reviewed in detail. The emphasis has been placed on newer insights in the virology, immunology, serology, epidemiology, and especially clinical aspects of human cytomegalovirus. I wish tobring out the interactions of knowledge in these areas. Part II is a much shorter treatment of the nonhuman cytomegaloviruses, reviewed primarily from the point of view of their contribution to the understanding of the human virus. V vi Preface to the First Edition Finally, I wish to thank Drs. Charles R. Rinaldo, Jr., John A. Armstrong, and Donald N. Medearis for their critical review of the manuscript. Their suggestions were invaluable. Dr. John N. Dowling's thorough review of CMV infection in patients with malignancies was the basis of Section 13.4. I also acknowledge the indispensable and patient secretarial assistance of Ms.The remarkable medical events and advances in knowle...

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Evaluation of a commercial latex agglutination test for detecting antibodies to cytomegalovirus in organ donors and transplant recipients

Cited by 29 publications

References 7 publications

Detection of latent human cytomegalovirus in organ tissue and the correlation with serological status

Detection of latent human cytomegalovirus in organ tissue and the correlation with serological status

The Presence of Cytomegalovirus Antigens in Karyotyped Abortions

Cytomegalovirus

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