Evaluation of 4 quantification methods for monitoring 16 antibiotics and 1 beta-lactamase inhibitor in human serum by high-performance liquid chromatography with tandem mass spectrometry detection

Seraissol, Patrick; Lanot, Thomas; Baklouti, S.; Mané, Camille; Ruiz, Stéphanie; Lavit, Michel; Riols, Pascale De; Garrigues, Jean‐Christophe; Gandia, Peggy

doi:10.1016/j.jpba.2022.114900

Cited by 15 publications

(12 citation statements)

References 27 publications

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“…We used a deuterated chemical analog of DBV as IS. This is in agreement with the study of Seraissol et al [ 21 ] and different from other methods published previously [ 17 , 18 , 19 ]. The major advantage granted by using a deuterated IS is the high accuracy achieved in compensating for extraction yield and matrix effect variations of patients’ samples whose content and composition of endogenous and exogenous compounds may be very different.…”

Section: Discussionsupporting

confidence: 93%

“…The aim of this study was to develop and validate a fast and simple analytical method for measuring DBV concentrations in plasma microsamples by means of a liquid chromatography-isotope dilution tandem mass spectrometry (ITD LC-MS/MS) technique using as internal standard the exa-deuterated analog of DBV (DBV-d6), similar to Seraissol et al [ 21 ].…”

Section: Introductionmentioning

confidence: 99%

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Fast and Simple Liquid Chromatography-Isotope Dilution Tandem Mass Spectrometry Method for Therapeutic Drug Monitoring of Dalbavancin in Long-Term Treatment of Subacute and/or Chronic Infections

et al. 2023

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Dalbavancin (DBV) is a long-acting antistaphylococcal lypoglycopeptide that is being increasingly used for long-term treatment of a wide range of subacute and/or chronic infections, mainly osteo-articular infections (OAI). Population pharmacokinetic studies showed that two 1500 mg doses 1 week apart can ensure effective treatment for several weeks. In this scenario, therapeutic drug monitoring (TDM) can be a helpful tool for providing clinicians with real-time feedback on the duration of optimal treatment by measuring drug concentrations over time in each single patient. The aim of this study was to develop and validate a fast and simple analytical method based on the Liquid Chromatography-Isotope Dilution Tandem Mass Spectrometry (ITD LC-MS/MS) technique for measuring DBV concentrations in human plasma microsamples. It will allow an innovative, very convenient and minimally invasive way of sampling. Analysis was performed by simple single-step sample preparation and very short instrumental run time (4 min). Analytical performance met all criteria in terms of specificity, sensitivity, linearity, precision, accuracy, matrix effect, extraction recovery, limit of quantification, dilution integrity and stability under different conditions set by the European Medicines Agency (EMA) for drug quantification by means of bioanalytical methods. The method was successfully applied for measuring DBV concentrations (range = 2.0–77.0 mg/L) in a cohort of patients receiving long-term DBV treatment of subacute and/or chronic infections.

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Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Fast and Simple Liquid Chromatography-Isotope Dilution Tandem Mass Spectrometry Method for Therapeutic Drug Monitoring of Dalbavancin in Long-Term Treatment of Subacute and/or Chronic Infections

et al. 2023

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“…HPLC can effectively exclude the influence of digoxin metabolites in patients on the detection results and has high specificity for digoxin. − The results obtained using this method were accurate and highly sensitive. However, it has a certain requirement for operational technology, and thus this method is preferred when the sample size is small. Radioimmunoassay (RIA) combines the high sensitivity of radionuclide tracing technology and the high specificity of immunochemistry.…”

Section: Resultsmentioning

confidence: 99%

Immunosensor for Rapid and Sensitive Detection of Digoxin

et al. 2023

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Digoxin is a cardiac glycosylated steroid-like drug with a positive inotropic effect and has been widely used in treating congestive heart failure, atrial fibrillation, atrial flutter, and other heart diseases. Digoxin is also a dangerous drug, which can cause drug poisoning at a low blood drug concentration (2.73–3.9 nmol/L, i.e., 2.14–3.05 ng/mL). Therefore, the timely detection of a patient’s blood drug concentration plays a significant role in controlling blood drug concentration, reducing the occurrence of drug poisoning events, and maximizing the role of drug therapy. In this study, a DNA vector for the expression of the antidigoxin antibody Fab fragment was constructed. With the vector, Fab was expressed in E. coli and purified, and 1.2 mg of antibodies was obtained from 100 mL of culture. An immunofluorescent sensor based on the mechanism of photoinduced electron transfer was constructed by labeling additional cysteines in the heavy chain variable region and light chain variable region of the antibody Fab fragment with fluorescent dyes. The assay for digoxin with the immunosensor could be finished within 5 min with a limit of detection of 0.023 ng/mL, a detectable range of 0.023 ng/mL to 100 μg/mL, and an EC50 of 0.256 ng/mL. A new approach for the rapid detection of digoxin was developed and will contribulte to therapeutic drug monitoring.

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Section: Beta-lactamsmentioning

confidence: 99%

“…47 In addition, difficulties such as the lack of commercial kits for beta-lactam serum measurement, absence of education on beta-lactam TDM, and low beta-lactam serum stability (eg, 3 hours for cefepime and piperacillin-tazobactam at ambient temperature) are important barriers to be considered in daily routine. 48 Currently, beta-lactam TDM is not widely used in clinical practice, and there is no conclusive analysis of its cost benefits. 49 An multicenter randomized clinical trial in the Netherlands (the DOLPHIN study) was designed to assess the efficacy and cost benefit of beta-lactam and fluoroquinolone TDM.…”

Section: Beta-lactamsmentioning

confidence: 99%

Optimization of Antimicrobial Stewardship Programs Using Therapeutic Drug Monitoring and Pharmacokinetics–Pharmacodynamics Protocols: A Cost-Benefit Review

Telles

Morales

Yamada

et al. 2023

Therapeutic Drug Monitoring

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Purpose:Antimicrobial stewardship programs are important for reducing antimicrobial resistance because they can readjust antibiotic prescriptions to local guidelines, switch intravenous to oral administration, and reduce hospitalization times. Pharmacokinetics–pharmacodynamics (PK-PD) empirically based prescriptions and therapeutic drug monitoring (TDM) programs are essential for antimicrobial stewardship, but there is a need to fit protocols according to cost benefits. The cost benefits can be demonstrated by reducing toxicity and hospital stay, decreasing the amount of drug used per day, and preventing relapses in infection. Our aim was to review the data available on whether PK-PD empirically based prescriptions and TDM could improve the cost benefits of an antimicrobial stewardship program to decrease global hospital expenditures.Methods:A narrative review based on PubMed search with the relevant studies of vancomycin, aminoglycosides, beta-lactams, and voriconazole.Results:TDM protocols demonstrated important cost benefit for patients treated with vancomycin, aminoglycosides, and voriconazole mainly due to reduce toxicities and decreasing the hospital length of stay. In addition, PK-PD strategies that used infusion modifications to meropenem, piperacillin-tazobactam, ceftazidime, and cefepime, such as extended or continuous infusion, demonstrated important cost benefits, mainly due to reducing daily drug needs and lengths of hospital stays.Conclusions:TDM protocols and PK-PD empirically based prescriptions improve the cost-benefits and decrease the global hospital expenditures.

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Evaluation of 4 quantification methods for monitoring 16 antibiotics and 1 beta-lactamase inhibitor in human serum by high-performance liquid chromatography with tandem mass spectrometry detection

Cited by 15 publications

References 27 publications

Fast and Simple Liquid Chromatography-Isotope Dilution Tandem Mass Spectrometry Method for Therapeutic Drug Monitoring of Dalbavancin in Long-Term Treatment of Subacute and/or Chronic Infections

Fast and Simple Liquid Chromatography-Isotope Dilution Tandem Mass Spectrometry Method for Therapeutic Drug Monitoring of Dalbavancin in Long-Term Treatment of Subacute and/or Chronic Infections

Immunosensor for Rapid and Sensitive Detection of Digoxin

Optimization of Antimicrobial Stewardship Programs Using Therapeutic Drug Monitoring and Pharmacokinetics–Pharmacodynamics Protocols: A Cost-Benefit Review

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