Evaluation of 4-nonylphenols and 4-tert-octylphenol contamination of fish in rivers by laboratory accumulation and excretion experiments

Tsuda, Taizo; Takino, Akihiko; Muraki, Kazue; Harada, Hiroyuki; Kojima, Mihoko

doi:10.1016/s0043-1354(00)00445-0

Cited by 51 publications

(18 citation statements)

References 26 publications

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“…Tsuda et al . reported NP bioconcentration factors of 167 in killifish and 21 in ayu fish 9 . Our results are in the same order as for these previous findings.…”

Section: Bioconcentration Factors Of Of Bisphenol a (Bpa) And Nonylphsupporting

confidence: 91%

Bisphenol A and nonylphenol bioconcentration in spotted halibut Varaspar variegates

et al. 2004

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KEY WORDS: bioconcentration factors, bisphenol A, nonylphenol, spotted halibut.Bisphenol A (2,2-bis(4-hydroxyphenyl)propane, BPA) and 4-nonylphenol (isomers mixture, NP) are synthetic chemicals used in a wide variety of plastics and other products, and many scientists have reported that these chemicals have an endocrinedisrupting effect. 1-4 High concentrations of BPA have been detected in industrial waste effluent. 5 In Japan, endocrine-disrupting chemicals have been detected in river water, seawater, fish and other aquatic organisms. 6,7 However, little information is available about uptake and accumulation of these chemicals from the environment on marine fish. In the present study, spotted halibut ( Varaspar variegates ) were exposed to waterborne BPA and NP in seawater for several days, and time dependence of BPA and NP concentrations in these fish was investigated.Spotted halibut (2 months old) were maintained in a water tank at our laboratory under natural photoperiod at a constant temperature of 14.0 ± 0.2 ∞ C for 2 weeks before the exposure experiment. The fish were starved during this preexperimental period. At the start of the experiment, 140 fish (body weight, 0.06-0.12 g; body length, 8-15 mm) were randomly selected, distributed into four groups of 35 fish, and placed into four glass tanks (30 L). During the 1-week experimental period, the fish were starved and exposed to waterborne BPA (Tokyo Kasai Kogyo Co., Ltd, Tokyo, Japan) and NP (isomers mixture; Kanto Chemical Co., Inc., Tokyo, Japan). The groups were exposed to the following concentration mixtures: group 1 (control), 0 m g/L BPA and 0 m g/L NP; group 2, 7 m g/L BPA and 8 m g/L NP; group 3, 70 m g/L BPA and 80 m g/L NP; group 4, 700 m g/L BPA and 800 m g/L NP. BPA and NP were dissolved in ethanol prior to being added to the tank water. The final concentration of ethanol in the water was 10 m L/L. Every 24 h during the experimental period, all of the water in each experimental tank was changed. Seven fish were taken out of each tank for analysis after 0, 1, 2, 4 and 7 days of exposure. To the samples were added the internal standards, BPA-d 16 and NP-d 4 (Kanto Chemical Co., Inc.), and they were homogenized with methanol. Then, the extracts were concentrated using flowing N 2 gas and cleaned using a silica-gel column by hexane. The chemicals, which were eluted with acetone : hexane (30% : 70%) were again concentrated with N 2 , and were then derivatized using N,O -bis(trimethylsilyl)trifluoroacetamide (BSTFA; SUPELCO, Inc., Bellefonte, PA, USA). Trimethylsilylated BPA (BPA-TMS) and NP (NP-TMS) were analyzed by gas chromatography/mass spectroscopy (GC/MS). GC/MS analysis was performed using a Varian CP-3800 GC and SATURN 2000 MS in EI auto mode with a scan range of 40-600 a.m.u. (Varian, Palo Alto, CA, USA). Chromatographic separations were performed using a 30 m ¥ 0.25 mm i.d. (film thickness, 0.25 m m) DB-5 capillary column (J & W Scientific, Palo Alto, CA, USA). The injector temperature was maintained at 280 ∞ C, and the injection volume was...

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“…Tsuda et al . reported NP bioconcentration factors of 167 in killifish and 21 in ayu fish 9 . Our results are in the same order as for these previous findings.…”

Section: Bioconcentration Factors Of Of Bisphenol a (Bpa) And Nonylphsupporting

confidence: 91%

Bisphenol A and nonylphenol bioconcentration in spotted halibut Varaspar variegates

et al. 2004

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“…Indeed, the assumption that total 4 NP amounts at the end of the depuration result from the metabolization of NP1EC leads to conclude that total 4 NP background amounts have been eliminated, which suggests a lower depuration halflife for 4 NP than for NP1EC. To compare, depuration halflives for 4 NP of 9 h (Tsuda et al 2001) and 62 h have been reported respectively for killifish and G. pulex (CrossSorokin et al 2003). Furthermore, 4 NP is commonly biotransformed in fish into its conjugated form by the glutathione-S-transferase enzyme (Hugues and Gallagher 2004) which has also been found in E. affinis (Cailleaud et al 2007c) and which could explain the elimination of 4 NP by this copepod.…”

Section: Npe Uptake and Depuration In E Affinismentioning

confidence: 99%

Uptake and elimination, and effect of estrogen-like contaminants in estuarine copepods: an experimental study

Cailleaud

Budzinski

Lardy

et al. 2010

Environ Sci Pollut Res

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“…Conversely, in the case of NP, the present experiment and published literature (e.g., Yokota et al, 2001;Kang et al, 2003;Ishibashi et al, 2006) demonstrate that concentrations p20 mg/L are below threshold for reproductive effects while reproductive LOAELs are in the 52-100 mg/L range for freshwater fish. Given that surface water NP concentrations are typically well below these toxic levels (see Section 1; Ahel et al, 1994;Blackburn and Waldock, 1995;Tsuda et al, 2001;Kolpin et al, 2002). However, NP is a degradation product of NPEOs and usually co-occurs with other often more potent alkylphenols (APs).…”

Section: Reproduction Growth and Survival Of Larvaementioning

confidence: 99%

“…Field surveys have revealed that nonylphenols are distributed at low mg/L levels in the aquatic environment (Tsuda et al, 2001). More specifically, the US Geological Survey reported that NP was one of the most frequently detected compounds with a median concentration of 0.8 mg/L (Kolpin et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Comparison of ethinylestradiol and nonylphenol effects on reproduction of Chinese rare minnows (Gobiocypris rarus)

Zha

Sun

Spear

et al. 2008

Ecotoxicology and Environmental Safety

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Breeding pairs of rare minnows were exposed to 5, 10, 20 mg/L 4-nonylphenol (NP) or 4 ng/L 17a-ethinylestradiol (EE 2 ) for 21 days. Among reproductive parameters investigated, fecundity, fertility, and laying interval were significantly affected by 4 ng/L EE 2 , but were not affected at any exposure level of NP. These effects coincided with male feminization, ova-testis, increased plasma VTG in both males and females as well as the accumulation of eosinophilic material in kidneys and livers. Histopathologic evaluation revealed severe kidney lesions in both EE 2 and NP-exposed fish. The bioassay succeeded in demonstrating that EE 2 was several orders of magnitude more potent than the industrial chemical NP. The rare minnow was more sensitive to EE 2 than medaka, but comparable to zebrafish in terms of sensitivity. The results of the present investigation indicated that a short-term reproductive bioassay is feasible using the Chinese rare minnow as the test species. r

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Evaluation of 4-nonylphenols and 4-tert-octylphenol contamination of fish in rivers by laboratory accumulation and excretion experiments

Cited by 51 publications

References 26 publications

Bisphenol A and nonylphenol bioconcentration in spotted halibut Varaspar variegates

Bisphenol A and nonylphenol bioconcentration in spotted halibut Varaspar variegates

Uptake and elimination, and effect of estrogen-like contaminants in estuarine copepods: an experimental study

Comparison of ethinylestradiol and nonylphenol effects on reproduction of Chinese rare minnows (Gobiocypris rarus)

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