Evaluation of 25-hydroxyvitamin D quantification using a commercial HPLC kit method

Dd, Heath; Flatt, Shirley W.; Ca, Thomson; Et, Jacobs; Ma, Pruitt; Cl, Rock

doi:10.1080/09674845.2011.11730329

Cited by 5 publications

(4 citation statements)

References 3 publications

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“…However, a liquid chromatography/mass spectrometry method to measure 25 OHD is currently a preferred technique. This technique has the ability to separate and accurately quantify both vitamin D 2 and vitamin D 3 , as compared with radioimmunoassay [56,57].…”

Section: Discussionmentioning

confidence: 99%

Less than Adequate Vitamin D Status and Intake in Latin America and the Caribbean: A Problem of Unknown Magnitude

Brito¹,

Cori²,

Olivares³

et al. 2013

Food Nutr Bull

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Section: Discussionmentioning

confidence: 99%

Less than Adequate Vitamin D Status and Intake in Latin America and the Caribbean: A Problem of Unknown Magnitude

Brito¹,

Cori²,

Olivares³

et al. 2013

Food Nutr Bull

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show abstract

“…Thus, for the current work, 25(OH)D was measured an average of 2 years post- diagnosis, similar to the study of Neuhouser et al(4). The chemiluminescent DiaSorin assay, a direct, competitive chemiluminescence immunoassay was employed for 25(OH)D analysis in the current work and has been described in detail elsewhere(3, 25, 26). Briefly, the assay range was 7.0 -150 ng/mL and the lowest reportable value was 7.0 ng/mL.…”

Section: Methodsmentioning

confidence: 99%

Correlates of 25-Hydroxyvitamin D and Breast Cancer Stage in the Women's Healthy Eating and Living Study

Jacobs

Thomson

Flatt

et al. 2013

Nutrition and Cancer

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Inverse associations between circulating 25-hydroxyvitamin D [25(OH)D] and breast cancer stage have been reported, thus it is critical to understand the variables that contribute to 25(OH)D levels among women with breast cancer. Among 904 women in the Women’s Healthy Eating and Living Study, plasma 25(OH)D concentrations were measured and data on demographic characteristics, diet, physical activity, and tumor characteristics were collected at study entry. Statistically significant associations with 25(OH)D concentrations were observed for body mass index (BMI), body surface area (BSA), height, smoking, total vitamin D intake, physical activity, and race or ethnicity. Of the correlates of 25(OH)D, BMI, BSA, height, physical activity, and study site were associated with stage of breast cancer; however, concentrations of 25(OH)D were not significantly related to stage. In fully adjusted logistic regression models, the ORs (95% CIs) for the association between vitamin D deficiency and Stage II and III cancers were 0.85 (0.59-1.22) and 1.23 (0.71-2.15), respectively (p-trend=0.59), compared to Stage I. This study confirms previous work regarding the correlates of 25(OH)D concentrations but does not provide support for an association between vitamin D status and breast cancer stage.

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“…Due to an increased awareness of vitamin D deficiency, clinical laboratories have seen significant increase in the testing of 25‐OH vitamin D2 and D3. The common methods for the measurement of vitamin D metabolites are immunoassays and chromatographic methods . Due to high sensitivity and specificity, and ability to differentiate between D2 and D3, liquid chromatography tandem mass spectrometry (LC‐MS‐MS) using electrospray or atmospheric pressure chemical ionization (APCI) is a preferred method.…”

Section: Introductionmentioning

confidence: 99%

A Simple, Rapid Atmospheric Pressure Chemical Ionization Liquid Chromatography Tandem Mass Spectrometry Method for the Determination of 25‐Hydroxyvitamin D2 and D3

Garg

Munar

Frazee

et al. 2012

Clinical Laboratory Analysis

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Vitamin D plays a vital role not only in bone health but also in pathophysiology of many other body functions. In recent years, there has been significant increase in testing of 25-hydroxyvitamin D (25-OH vitamin D), a marker of vitamin D deficiency. The most commonly used methods for the measurement of 25-OH vitamin D are immunoassays and liquid chromatography tandem mass spectrometry (LC-MS-MS). Since immunoassays suffer from inaccuracies and interferences, LC-MS-MS is a preferred method. In LC-MS-MS methods, 25-OH vitamin D is extracted from serum or plasma by solid-phase or liquid-phase extraction. Because these extraction methods are time consuming, we developed an easy method that uses simple protein precipitation followed by injection of the supernatant to LC-MS-MS. Several mass-to-charge (m/z) ratio transitions, including commonly used transitions based on water loss, were evaluated and several tube types were tested. The optimal transitions for 25-OH vitamin D2 and D3 were 395.5 > 269.5 and 383.4 > 257.3, respectively. The reportable range of the method was 1-100 ng/mL, and repeatability (within-run) and within-laboratory imprecision were <4% and <6%, respectively. The method agreed well with the solid-phase extraction methods.

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Evaluation of 25-hydroxyvitamin D quantification using a commercial HPLC kit method

Cited by 5 publications

References 3 publications

Less than Adequate Vitamin D Status and Intake in Latin America and the Caribbean: A Problem of Unknown Magnitude

Less than Adequate Vitamin D Status and Intake in Latin America and the Caribbean: A Problem of Unknown Magnitude

Correlates of 25-Hydroxyvitamin D and Breast Cancer Stage in the Women's Healthy Eating and Living Study

A Simple, Rapid Atmospheric Pressure Chemical Ionization Liquid Chromatography Tandem Mass Spectrometry Method for the Determination of 25‐Hydroxyvitamin D2 and D3

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