Evaluation of 18F-labeled targeted perfluorocarbon-filled albumin microbubbles as a probe for microUS and microPET in tumor-bearing mice

Liao, Ai‐Ho; Wu, Shih-Yen; Wang, Hsin‐Ell; Weng, Chien-Hsiu; Wu, Ming‐Fang; Li, Pai‐Chi

doi:10.1016/j.ultras.2012.06.014

Cited by 20 publications

(14 citation statements)

References 28 publications

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“… Figure 1 illustrates the TEM image of albumin MBs. The shell thickness of the albumin MBs was about 30–60 nm [23]. For albumin/dextrose MB preparation, dextrose (D(+)-glucose; Acros Organics, Fair Lawn, NJ, USA) was purchased to prepare stock solutions of 5%, 10%, 15%, 20%, 30%, 40%, and 45% (weight (w)/volume (v)) dextrose in physiological saline (pH 7.4, 0.9% sodium chloride).…”

Section: Methodsmentioning

confidence: 99%

Effects of Microbubble Size on Ultrasound-Mediated Gene Transfection in Auditory Cells

Liao

Hsieh

Hsin-Chiao

et al. 2014

BioMed Research International

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Gene therapy for sensorineural hearing loss has recently been used to insert genes encoding functional proteins to preserve, protect, or even regenerate hair cells in the inner ear. Our previous study demonstrated a microbubble- (MB-)facilitated ultrasound (US) technique for delivering therapeutic medication to the inner ear. The present study investigated whether MB-US techniques help to enhance the efficiency of gene transfection by means of cationic liposomes on HEI-OC1 auditory cells and whether MBs of different sizes affect such efficiency. Our results demonstrated that the size of MBs was proportional to the concentration of albumin or dextrose. At a constant US power density, using 0.66, 1.32, and 2.83 μm albumin-shelled MBs increased the transfection rate as compared to the control by 30.6%, 54.1%, and 84.7%, respectively; likewise, using 1.39, 2.12, and 3.47 μm albumin-dextrose-shelled MBs increased the transfection rates by 15.9%, 34.3%, and 82.7%, respectively. The results indicate that MB-US is an effective technique to facilitate gene transfer on auditory cells in vitro. Such size-dependent MB oscillation behavior in the presence of US plays a role in enhancing gene transfer, and by manipulating the concentration of albumin or dextrose, MBs of different sizes can be produced.

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Section: Methodsmentioning

confidence: 99%

Effects of Microbubble Size on Ultrasound-Mediated Gene Transfection in Auditory Cells

Liao

Hsieh

Hsin-Chiao

et al. 2014

BioMed Research International

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“…MBs were injected into the tail vein while imaging the mouse. Nontargeting MBs were cleared by the bloodstream very quickly (typically within 5–10 minutes)16. Targeting MBs (tMBs), on the other hand, bind to the specific angiogenesis marker and thus stay at the tumor site over a longer period of time.…”

Section: Resultsmentioning

confidence: 99%

“…Moreover, the use of molecular binding extends the retention period of these targeted AuMBs in the tumor relative to using plain MBs (i.e., without bioconjugation). MB accumulation can be observed using ultrasound imaging (i.e., ultrasound molecular imaging)16.…”

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confidence: 99%

Synergistic delivery of gold nanorods using multifunctional microbubbles for enhanced plasmonic photothermal therapy

Wang

Chen

Liao

et al. 2014

Sci Rep

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Plasmonic photothermal therapy (PPTT) using plasmonic nanoparticles as efficient photoabsorbing agents has been proposed previously. One critical step in PPTT is to effectively deliver gold nanoparticles into the cells. This study demonstrates that the delivery of gold nanorods (AuNRs) can be greatly enhanced by combining the following three mechanisms: AuNRs encapsulated in protein-shell microbubbles (AuMBs), molecular targeting, and sonoporation employing acoustic cavitation of microbubbles (MBs). Both in vitro and in vivo tests were performed. For molecular targeting, the AuMBs were modified with anti-VEGFR2. Once bound to the angiogenesis markers, the MBs were destroyed by ultrasound to release the AuNRs and the release was confirmed by photoacoustic measurements. Additionally, acoustic cavitation was induced during MB destruction for sonoporation (i.e., increase in transient cellular permeability). The measured inertial cavitation dose was positively correlated with the temperature increase at the tumor site. The quantity of AuNRs delivered into the cells was also determined by measuring the mass spectrometry and observed using third-harmonic-generation microscopy and two-photon fluorescence microscopy. A temperature increase of 20°C was achieved in vitro. The PPTT results in vivo also demonstrated that the temperature increase (>45°C) provided a sufficiently high degree of hyperthermia. Therefore, synergistic delivery of AuNRs was demonstrated.

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“…66 Other applications include better characterizing phantom tissue, 154 tracking cell death from macro-to submicroscales, 67,92,155 detecting metastatic regions in lymph nodes, 156 and characterizing contrast agent biodistribution. 93 The aforementioned studies are the first few to explore this modality with QMIB, with many more potential opportunities. The parameters that HF-US measures reflect the tissue microstructure, 47,137,141,157,158 which is important in breast cancer development and progression.…”

Section: High-frequency Ultrasoundmentioning

confidence: 99%

Review of quantitative multiscale imaging of breast cancer

et al. 2018

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Breast cancer is the most common cancer among women worldwide and ranks second in terms of overall cancer deaths. One of the difficulties associated with treating breast cancer is that it is a heterogeneous disease with variations in benign and pathologic tissue composition, which contributes to disease development, progression, and treatment response. Many of these phenotypes are uncharacterized and their presence is difficult to detect, in part due to the sparsity of methods to correlate information between the cellular microscale and the whole-breast macroscale. Quantitative multiscale imaging of the breast is an emerging field concerned with the development of imaging technology that can characterize anatomic, functional, and molecular information across different resolutions and fields of view. It involves a diverse collection of imaging modalities, which touch large sections of the breast imaging research community. Prospective studies have shown promising results, but there are several challenges, ranging from basic physics and engineering to data processing and quantification, that must be met to bring the field to maturity. This paper presents some of the challenges that investigators face, reviews currently used multiscale imaging methods for preclinical imaging, and discusses the potential of these methods for clinical breast imaging.

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Evaluation of 18F-labeled targeted perfluorocarbon-filled albumin microbubbles as a probe for microUS and microPET in tumor-bearing mice

Cited by 20 publications

References 28 publications

Effects of Microbubble Size on Ultrasound-Mediated Gene Transfection in Auditory Cells

Effects of Microbubble Size on Ultrasound-Mediated Gene Transfection in Auditory Cells

Synergistic delivery of gold nanorods using multifunctional microbubbles for enhanced plasmonic photothermal therapy

Review of quantitative multiscale imaging of breast cancer

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