Evaluation of 177Lu[Lu]-CHX-A″-DTPA-6A10 Fab as a radioimmunotherapy agent targeting carbonic anhydrase XII

Fully Automated Production and Characterization of 64Cu and Proof‐of‐Principle Small‐Animal PET Imaging Using 64Cu‐Labelled CA XII Targeting 6A10 Fab

“…The image also shows activity accumulation in organs such as kidney,l iver,b ladder,s pleen and intestines. According to our previous work, [60] this accumulationp attern can be expected, as the metabolism of proteins with molecular weights imilar to aF ab fragment (~55 kDa) is dominated by kidney and liver.T he calculated tumor-to-contralateral muscle ratio of 12 is notably high for as ystemically applied Fab fragment, as Fabs undergo rapid degradation processes and are therefore much more short-lived in the blood than full-length antibodies. [10][11][12] This encourages further studies regarding the in vivo behavior of this 64 Cu-labelled compound for diagnostic imaging of CA XII expressing tumors.…”

“…The cobalt isotopes 55 Co, 57 Co and 61 Co were identifiedw ith contentso f0 .03 AE 0.10, 0.004 AE 0.014 and 1.4 AE 3.4 At %, respectively.T he amount of 64 Cu was determined to be 98.5 AE 3.4 At %. Radionuclides with short half-lives, such as [60][61][62] Cu, were not detected in the examined sample. This supports the implementation of a1 2h time intervalb etweenE OB and EOP for eliminationo fa ny short-lived isotopes.…”

“…This enzyme is expressed on various aggressively growing cancer cells, such as renal carcinoma, breast cancer, ovarian tumors, and gliomas . The 6A10 Fab has already been radiolabelled with 177 Lu and investigated for potential use as a radiotherapy agent against glioma recurrence . Further applications as a diagnostic tracer, radiolabelled with the PET nuclide 64 Cu, is now considered to extend the use of this biomolecule for tumor imaging.…”

“…[59] The 6A10 Fab has already been radiolabelledw ith 177 Lu and investigated for potentialu se as a radiotherapy agent against glioma recurrence. [60] Further applications as ad iagnostic tracer,r adiolabelled with the PET nuclide 64 Cu, is now considered to extendt he use of this biomolecule for tumor imaging.…”

Targeted RNA NextGenSeq profiling in oncology using single molecule molecular inversion probes

“…How the lack of exon 9 in CA12v2 affects its function is not known. Because CA12v2 is not expressed in normal brain, it may be a good target for glioblastomas expressing this variant [60]. Other interesting therapeutic targets for low-grade gliomas that were identified by our assay are NTRK2, ERBB3 and ERBB4.…”