“…Each tooth was first reduced to powder. After overnight decalcification and protein digestion at 558C with agitation (EDTA 0.5 M, pH 5 8.5, proteinase K 1-2 mg/mL, N-lauryl Sarcosyl 0.5%), the supernatants were extracted using phenol-chloroform- Ubelaker, 1984; sex determination according to Bruzek et al, 1996;n.d Amplification, cloning, and sequencing PCR amplifications were performed on the HVR-I region of the mitochondrial DNA control region. Partial sequences of 225 bp of the HVR-I (nps 16,165 to 16,390) were obtained using a set of three overlapping primer pairs: Ms1 (Jehaes et al, 2001), L16210 (nps 16,190-16,210) and H16322 (nps 16,302-16,322), and Ms3 (Jehaes et al, 2001).…”