Évaluation de la stratégie de dépistage du virus de l’hépatite B chez les donneurs de sang à Brazzaville

Angounda, Brunel Monic; Dzia, Amélia Bokilo; Niama, Fabien Roch; Ibara, Blaise Irénée Atipo; Kobawila, Simon Charles; Ibara, J. R.

doi:10.1016/j.tracli.2015.06.045

Cited by 1 publication

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“…This male superiority, is explained by the many contraindications to blood donation in women (breastfeeding, pregnancy and menstruation). This is in line with several studies carried out at the CNTS [21][22][23][24].…”

Section: Discussionsupporting

confidence: 92%

Gene expression profiling of p53 and c-myc in HTLV-1 positive blood donors in Congo

Iloukou,

Boumba,

Pouki

et al. 2024

Problems of Virology

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Objectives. The HTLV-1 infection persists for life, remaining as asymptomatic viral reservoirs in most patients, ensuring the chain of transmission, but around 4% develop adult T-cell leukemia/lymphoma (ATLL). HTLV-1 is an oncogenic retrovirus that transforms CD4+ T lymphocytes and deregulates the lymphoproliferative pathways that contribute to the development of ATLL. To achieve cell transformation, most oncogenic retroviruses use proto-oncogene capture transduction, with proviral integration disrupting the expression of tumor suppressors or proto-oncogenes. The aim. We conducted this study on the prevalence of HTLV-1 infection in blood donors to expand the HTLV-1 database, assess the risk of transmission via blood products, as well as evaluate the risk of persistent infection or development of neoplastic diseases in HTLV-1 carriers. Materials and methods. This is a cross-sectional study of blood donors of all categories. For this study, 265 blood donors were recruited at the Centre National de Transfusion Sanguine in Brazzaville. After testing for HTLV-1 antibodies by ELISA, proviral DNA was extracted from all ELISA-positive samples for detection by nested PCR, followed by RT qPCR using specific primers p53 and c-myc for gene expression. Results. 20/265 were positive for anti-HTLV-1 antibody, 5 donors were positive for proviral DNA. The prevalence of HTLV-1 was 1.8%. All HTLV-1-positive donors were male (1.8%), with a positive correlation (p = 0.05); the 1.1% of positive donors were regular, with the majority aged between 31 and 45 years (1.5%), and concubine donors were the most frequent (1.1%). All samples showed normal expression of the p53 and c-myc genes. Conclusion. The prevalence, though low, remains a serious problem. No abnormal p53 or c-myc gene expression was detected in HTLV-1-positive donors, which could mean that none of the T lymphocytes in these donors had been transformed by HTLV-1.

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Section: Discussionsupporting

confidence: 92%