Evaluation criteria for bioaerosol samplers

Kesavan, Jana; Sagripanti, José-Luis

doi:10.1039/c4em00510d

Cited by 30 publications

(33 citation statements)

References 22 publications

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“…That work focused mainly on sampling efficiency associated with particle size and concentration, meteorological conditions, and/or culturability. Some studies focused on targeting specific microorganisms or on one type of air sampler (43,47,48,(64)(65)(66)(67)(68)(69)(70). Since the rise in popularity of sequencing techniques, bioinformatics analyses have allowed for a better understanding of the microbial composition and dynamics in several different environments.…”

Section: Discussionmentioning

confidence: 99%

Bioaerosol Sampler Choice Should Consider Efficiency and Ability of Samplers To Cover Microbial Diversity

Mbareche

Veillette

Bilodeau

et al. 2018

Appl Environ Microbiol

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Bioaerosol studies tend to describe the microbial content and understand the aerosolization processes linked to diseases. Air samplers are used to collect, identify and quantify bioaerosols. Studies comparing the performance of air samplers have typically used a culture approach or have targeted a specific microorganism in laboratory settings. The objective of this study was to use environmental field samples to compare the efficiency of 3 high airflow rate samplers for describing bioaerosol diversity using a next-generation sequencing approach. Two liquid cyclonic impactors and one electrostatic filter dry sampler were used in four wastewater treatment plants to target bacterial diversity and in five dairy farms to target fungal diversity. The dry electrostatic sampler was consistently more powerful in collecting more fungal and bacterial OTUs. Substantial differences in OTU abundance between liquid and dry sampling were revealed. The majority of the diversity revealed by dry electrostatic sampling was not identified using the cyclonic liquid impactors. The findings on this work suggest that the choice of a bioaerosol sampler should include information about the efficiency and ability of samplers to cover microbial diversity. Although these results suggest that electrostatic filters result in better coverage of the microbial diversity amongst the tested air samplers, further studies are needed to confirm this hypothesis. While it is difficult to determine a single universally optimal air sampler, this work provides an in-depth look at some of the considerations that are essential when choosing an air sampler for studying the microbial ecology of bioaerosols. Associating bioaerosol exposure and health problems is challenging and adequate exposure monitoring is a priority for scientist in the field. Conclusions that can be drawn from bioaerosol exposure studies are highly dependent on the design of the study and the methodologies used. The air sampling strategy is the first methodological step leading to an accurate interpretation of what is present in the air. Applying new molecular approaches to evaluate the efficiency of the different types of samplers used in the field is a necessary work around the traditional approaches and the biases they introduce to such studies. The results and conclusions provided in this manuscript should be taken in considerations when conducting a bioaerosol study.

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Section: Discussionmentioning

confidence: 99%

Bioaerosol Sampler Choice Should Consider Efficiency and Ability of Samplers To Cover Microbial Diversity

Mbareche

Veillette

Bilodeau

et al. 2018

Appl Environ Microbiol

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“…The VBCS samples at a flow rate of 25 L/min. We selected a flow rate of 25 L/ min to provide a balance of high enough flow rate to detect a short-term incident of bioaerosol while allowing the design of equipment to minimize stress that the microbes may experience from high-volume sampling (e.g., 500 L/min; Kesavan and Sagripanti 2015;Li et al 1999). The aerosol particles, including bioaerosols, are collected onto a 25 mm PU nanofiber filter held in a standard air sampling cassette.…”

Section: The Viable Bioaerosol Collection System (Vbcs)mentioning

confidence: 99%

“…Traditional impingers are limited to sampling times of less than 30 minutes (Lin et al 1997). Impingers, impactors, and cyclones have poor physical collection efficiencies for sizes below approximately 0.3-0.7 mm (Kesavan and Sagripanti 2015;Nevalainen et al 1993). Loading of the collection liquid with particles and evaporation of fluid (unless refreshed) are common problems for most liquid-based samplers.…”

Section: Introductionmentioning

confidence: 99%

Long-term viable bioaerosol sampling using a temperature- and humidity-controlled filtration apparatus, a laboratory investigation using culturable E. coli.

Walls

Kim

Yaga

et al. 2017

Aerosol Science and Technology

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2017) Longterm viable bioaerosol sampling using a temperature-and humidity-controlled filtration apparatus, a laboratory investigation using culturable E.coli., Aerosol Science and Technology, 51:5, 576-586, ABSTRACT Sampling for culturable (e.g., viable) aerosolized microbes (bioaerosols) is a useful means to provide information for public health monitoring and studies. However, it is challenging to maintain microbe culturability when sampling at high flow rates (>12 L/min) and extended periods of time (4 h). We developed a first-generation, viable bioaerosol collection system (VBCS) utilizing temperature (T) and relative humidity (RH)-conditioned filtration at a flow rate of 25 L/min. A twostage system of tube-in-shell Nafion TM exchange units provides cooling to 10 C and RH conditioning to 80-95%. Aerosol particles are collected on a polyurethane nanofiber filter providing a physical collection efficiency of >95% for sizes 0.06-10 mm. The T and RH conditions at the collection filter are maintained, despite changes to ambient conditions. The initial testing of the VBCS was done under indoor, laboratory conditions with aerosolized, vegetative E. coli. A scenario of a 30-min challenge of bioaerosol followed by continued sampling of clean air for various times was used to judge culturability maintenance under extended-term sampling. An initial loss of culturability upon collection onto the filter was observed; 23 § 13% relative to 4-mm all-glass impinger. However once collected, 98% of culturability was maintained for an additional 4.5 h of sampling. An exponential decay in culturability was observed from 8 h to 15 h of sampling. Also, 24h cold storage of the filters collected was studied. The VBCS is based on the use of dry filter cassettes, needs minimal maintenance, and preserves culturability of vegetative bacteria for >4 h. EDITOR Tiina Reponen

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“…Some examples of commercially available sample collectors include viable particle size samplers (impactors), virtual impactors, cyclones, and bubblers/impingers. Selection of samplers for BWA detection is very important as inappropriate selection could result in failure to collect particles of interest 72 . Sampling methods like wipe, wet/dry swab vacuum shock and composite-based sampling have been validated for collection of BWA samples from porous and nonporous surfaces…”

Section: Samplers/collectorsmentioning

confidence: 99%

Biological Warfare Agents and their Detection and Monitoring Techniques (Review Paper)

Pal¹,

Sharma²,

Sharma³

et al. 2016

Def. Sc. Jl.

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Recently, threat from biological warfare agents (BWAs) has emerged as the foremost national and global security challenge because of their simple and cheap production, easy dispersal, complicated detection, expensive protection and psychological, economical and social impact. Early detection and identification of BWAs during intentional biological event is essential to initiate corrective emergency responses for management of such incidents. Efforts are being made across the globe for development of state of the art technologies and systems for detection and identification of BWAs. However, till date there is no single system which can detect all the bio-threat agents. In the present review, we describe the currently available techniques and systems for detection and identification of these agents. The basic identification techniques including biological culture, immunological methods, nucleic acid based detection, MALDI-TOF MS, cellular fatty acid profiling and flow cytometry based detection are presented. Detection of BWAs with bio-sensors, surface plasmon resonance, biological detectors, and stand-off detection systems is also summarized. However, despite of availability of several techniques and tools, no full proof system is available for detection/identification of all the BWAs.

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Evaluation criteria for bioaerosol samplers

Cited by 30 publications

References 22 publications

Bioaerosol Sampler Choice Should Consider Efficiency and Ability of Samplers To Cover Microbial Diversity

Bioaerosol Sampler Choice Should Consider Efficiency and Ability of Samplers To Cover Microbial Diversity

Long-term viable bioaerosol sampling using a temperature- and humidity-controlled filtration apparatus, a laboratory investigation using culturable E. coli.

Biological Warfare Agents and their Detection and Monitoring Techniques (Review Paper)

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