Evaluation and identification of markers of damage in mushrooms (Agaricus bisporus) postharvest using a GC/MS metabolic profiling approach

O’Gorman, Aoife; Barry‐Ryan, Catherine; Frias, Jesus Maria

doi:10.1007/s11306-011-0294-3

Cited by 28 publications

(16 citation statements)

References 35 publications

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“…Testing A. bisporus , fatty acids were found to be the most important group of metabolites for predicting mechanical damage of mushrooms. The ratio between linolenic acid and pentadecanoic acid seems to be a useful chemometric tool …”

Section: Chemical Composition and Nutritional Valuementioning

confidence: 99%

“…The ratio between linolenic acid and pentadecanoic acid seems to be a useful chemometric tool. 28 Lipids of Macrolepiota procera and Collybia platyphylla provoked an increase in erythrocyte membrane fluidity, a property beneficial for hypertension treatment. 29 The nutritional contribution of mushroom lipids is limited due to low total lipid content and a low proportion of desirable n-3 fatty acids.…”

Section: Lipidsmentioning

confidence: 99%

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A review of chemical composition and nutritional value of wild‐growing and cultivated mushrooms

2012

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Fruit bodies of about 200 mushroom species are consumed throughout the world, preferably as a delicacy. Knowledge of their chemical composition, nutritional value and health-promoting effects has expanded dynamically during the last few years. Dry matter (DM) is low: commonly about 100 g kg⁻¹ . The usual contents of protein, lipids and ash are 200-250, 20-30 and 80-120 g kg⁻¹ DM, respectively. Various carbohydrates form the remaining DM. Nevertheless, great variations occur both among and within species. Energy is low, usually 350-400 kcal kg⁻¹ of fresh fruit bodies. The nutritional contribution of mushroom protein derived from earlier data seems to be overestimated. Fat content is low with markedly prevailing in linoleic acid and oleic acid, while the proportion of n-3 fatty acids is nutritionally marginal. The main carbohydrates are chitin, glycogen, trehalose and mannitol. Information on fibre content and composition is limited. Health-promoting β-glucans are an auspicious group of polysaccharides. High potassium content is characteristic of mushrooms. Several species can accumulate very high levels of both detrimental trace elements, particularly cadmium and mercury, and radiocaesium isotopes if growing on heavily polluted substrates. Mushrooms seem to be a considerable source of ergosterol, provitamin D₂, and phenolids with antioxidative properties. Hundreds of flavour constituents have been identified, particularly with eight-carbon aliphatic chains. Data on changes of mushroom components under various preservation conditions and culinary treatments have been fragmentary. Even more limited is knowledge of nutrient bioavailability.

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Section: Chemical Composition and Nutritional Valuementioning

confidence: 99%

Section: Lipidsmentioning

confidence: 99%

A review of chemical composition and nutritional value of wild‐growing and cultivated mushrooms

2012

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“…GC/MS and LC/MS complement each other, and a combination of these methods allows more comprehensive analysis of different compounds (Lee et al, 2013 ; Yao et al, 2013 ). The combination of the two methods have also been widely used to detect metabolites in mushrooms, such as P. ostreatus (Zhang et al, 2008 ), Agaricus bisporus (O'Gorman et al, 2011 ), and Lentinula edodes (Mata et al, 2014 ).…”

Section: Introductionmentioning

confidence: 99%

High-Temperature Induced Changes of Extracellular Metabolites in Pleurotus ostreatus and Their Positive Effects on the Growth of Trichoderma asperellum

Qiu

Zhang

et al. 2018

Front. Microbiol.

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Pleurotus ostreatus is a widely cultivated edible fungus in China. Green mold disease of P. ostreatus which can seriously affect yield is a common disease during cultivation. It occurs mostly after P. ostreatus mycelia have been subjected to high temperatures. However, little information is available on the relationship between high temperature and green mold disease. The aim of this study is to prove that extracellular metabolites of P. ostreatus affected by high temperature can promote the growth of Trichoderma asperellum. After P. ostreatus mycelia was subjected to high temperature, the extracellular fluid of P. ostreatus showed a higher promoting effect on mycelial growth and conidial germination of T. asperellum. The thiobarbituric acid reactive substance (TBARS) content reached the maximum after 48 h at 36°C. A comprehensive metabolite profiling strategy involving gas chromatography-mass spectrometry (GC/MS) combined with liquid chromatography-mass spectrometry (LC/MS) was used to analyze the changes of extracellular metabolites in response to high temperature. A total of 141 differential metabolites were identified, including 84.4% up-regulated and 15.6% down-regulated. Exogenous metabolites whose concentrations were increased after high temperature were randomly selected, and nearly all of them were able to promote the mycelial growth and conidial germination of T. asperellum. The combination of all selected exogenous metabolites also has the promotion effects on the mycelial growth and conidial germination of T. asperellum in a given concentration range in vitro. Overall, these results provide a first view that high temperature affects the extracellular metabolites of P. ostreatus, and the extensive change in metabolites promotes T. asperellum growth.

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“…Agaricus bisporus is highly susceptible to mechanical damage during storage or transportation. GC-MS technology has been used to identify metabolites that can be used as markers for A. bisporus injury (O’Gorman et al, 2012). Qiu et al (2018) used GC-MS and LC-MS to analyze changes in extracellular metabolites in the mycelium of P. ostreatus under high temperature conditions, as well as to study the effect of exogenous addition of some significantly increased metabolites on the growth of Trichoderma asperellum , with the goal of determining why P. ostreatus was easily infected by T .…”

Section: Introductionmentioning

confidence: 99%

Metabolic Profiling of Pleurotus tuoliensis During Mycelium Physiological Maturation and Exploration on a Potential Indicator of Mycelial Maturation

Zou

et al. 2019

Front. Microbiol.

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Pleurotus tuoliensis is a valuable and rare edible fungus with extremely high nutritional and medicinal value. However, the relative immaturity of P. tuoliensis cultivation technology leads to fluctuating yields and quality. The main difficulty in P. tuoliensis cultivation is estimate of mycelial maturity. There is currently no measurable indicator that clearly characterizes the physiological maturation of mycelia. The aim of this study was to identify potential indicators of physiological maturation for P. tuoliensis mycelia by using metabolomics approach. A metabolite profiling strategy involving gas chromatography-mass spectrometry (GC/MS) was used to analyze changes to extracellular metabolites in mycelia collected at mycelium physiological maturation period (MPMP) day 0, MPMP day 35 at 17°C and MPMP day 35 at 29°C. 72 differential metabolites (37.8% up-regulated and 62.2% down-regulated) were identified based on the selected criteria [variable important in projection (VIP) greater than 1.0 and p < 0.01]. Metabolic pathways enrichment analysis showed that these metabolites are involved in glycolysis, organic acid metabolism, amino acid metabolism, tricarboxylic acid cycle (TCA), sugar metabolism, nicotinate and nicotinamide metabolism, and oxidative phosphorylation. In addition, the pyrimidine synthesis pathway was significantly activated during mycelium physiological maturation of P. tuoliensis. The abundance of N-carbamoyl-L-aspartate (CA-asp), a component of this pathway, was significantly increased at MPMP day 35, which motivated us to explore its potential as an indicator for physiological maturation of mycelia. The content of CA-asp in mycelia changed in a consistent manner during physiological maturation. The feasibility of using CA-asp as an indicator for mycelial maturation requires further investigation.

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Evaluation and identification of markers of damage in mushrooms (Agaricus bisporus) postharvest using a GC/MS metabolic profiling approach

Cited by 28 publications

References 35 publications

A review of chemical composition and nutritional value of wild‐growing and cultivated mushrooms

A review of chemical composition and nutritional value of wild‐growing and cultivated mushrooms

High-Temperature Induced Changes of Extracellular Metabolites in Pleurotus ostreatus and Their Positive Effects on the Growth of Trichoderma asperellum

Metabolic Profiling of Pleurotus tuoliensis During Mycelium Physiological Maturation and Exploration on a Potential Indicator of Mycelial Maturation

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