Evaluation and identification of candidate genes for artificial microRNA-mediated resistance to tomato spotted wilt virus

Mitter, Neena; Zhai, Ying; Bai, Anh Xu; Chua, Keith; Eid, Sahar; Constantin, Myrna; Mitchell, Richard R.; Pappu, H. R.

doi:10.1016/j.virusres.2015.10.003

Cited by 37 publications

(14 citation statements)

References 54 publications

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“…The economic impact of TSWV has increased during the last decades, causing high yield losses in a variety of plant species, including economically important crops such as tomato and pepper (Turina et al, 2016). Recently, highly specific approaches based on the expression of artificial sRNA transgenes have been reported in model plants such as N. benthamiana or Nicotiana tabacum (Mitter et al, 2016;. Here, we aimed to systematically analyze and compare the anti-TSWV resistance induced by the expression of amiRNAs and syn-tasiRNAs in the natural hostof TSWV, S. lycopersicum.…”

Section: Discussionmentioning

confidence: 99%

Multi‐targeting of viral RNAs with synthetic trans‐acting small interfering RNAs enhances plant antiviral resistance

2019

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Summary RNA interference (RNAi)‐based tools are used in multiple organisms to induce antiviral resistance through the sequence‐specific degradation of target RNAs by complementary small RNAs. In plants, highly specific antiviral RNAi‐based tools include artificial microRNAs (amiRNAs) and synthetic trans‐acting small interfering RNAs (syn‐tasiRNAs). syn‐tasiRNAs have emerged as a promising antiviral tool allowing for the multi‐targeting of viral RNAs through the simultaneous expression of several syn‐tasiRNAs from a single precursor. Here, we compared in tomato plants the effects of an amiRNA construct expressing a single amiRNA and a syn‐tasiRNA construct expressing four different syn‐tasiRNAs against Tomato spotted wilt virus (TSWV), an economically important pathogen affecting tomato crops worldwide. Most of the syn‐tasiRNA lines were resistant to TSWV, whereas the majority of the amiRNA lines were susceptible and accumulated viral progenies with mutations in the amiRNA target site. Only the two amiRNA lines with higher amiRNA accumulation were resistant, whereas resistance in syn‐tasiRNA lines was not exclusive of lines with high syn‐tasiRNA accumulation. Collectively, these results suggest that syn‐tasiRNAs induce enhanced antiviral resistance because of the combined silencing effect of each individual syn‐tasiRNA, which minimizes the possibility that the virus simultaneously mutates all different target sites to fully escape each syn‐tasiRNA.

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Section: Discussionmentioning

confidence: 99%

Multi‐targeting of viral RNAs with synthetic trans‐acting small interfering RNAs enhances plant antiviral resistance

2019

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“…The transgenic rice plants showed high resistance simultaneously against RSV and RBSDV infection at a low temperature ( Sun et al, 2016 ). Thus far, engineering of amiRNA for antiviral resistance has been used successfully in various plant species, including N. benthamiana ( Qu et al, 2007 ; Ai et al, 2011 ; Kung et al, 2012 ; Ali et al, 2013 ; Song et al, 2014 ; Mitter et al, 2016 ; Wagaba et al, 2016 ; Carbonell and Daros, 2017 ), Arabidopsis ( Duan et al, 2008 ; Lin et al, 2009 ), rice ( Sun et al, 2016 ), wheat ( Fahim et al, 2012 ), maize ( Xuan et al, 2015 ), tomato ( Zhang et al, 2011 ; Vu et al, 2013 ), and grapevine ( Jelly et al, 2012 ) ( Table 1 ). Apart from being used in plant antiviral immune systems, engineering of amiRNA has been extensively applied in plant resistance against other pathogens such as bacteria ( Navarro et al, 2006 ; Li et al, 2010 ; Boccara et al, 2014 ; Ma et al, 2014 ), and fungi ( Liu et al, 2014 ; Ouyang et al, 2014 ; Xu et al, 2014 ).…”

Section: The Application Of Mirnas In Plant–virus Interactionsmentioning

confidence: 99%

MicroRNA-Mediated Gene Silencing in Plant Defense and Viral Counter-Defense

Liu

Zhou

et al. 2017

Front. Microbiol.

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MicroRNAs (miRNAs) are non-coding RNAs of approximately 20–24 nucleotides in length that serve as central regulators of eukaryotic gene expression by targeting mRNAs for cleavage or translational repression. In plants, miRNAs are associated with numerous regulatory pathways in growth and development processes, and defensive responses in plant–pathogen interactions. Recently, significant progress has been made in understanding miRNA-mediated gene silencing and how viruses counter this defense mechanism. Here, we summarize the current knowledge and recent advances in understanding the roles of miRNAs involved in the plant defense against viruses and viral counter-defense. We also document the application of miRNAs in plant antiviral defense. This review discusses the current understanding of the mechanisms of miRNA-mediated gene silencing and provides insights on the never-ending arms race between plants and viruses.

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“…Transgenic plants expressing artificial microRNAs (amiRNAs) have successfully been used to provide tolerance or resistance to virus infections caused by begomo-, cucumo-and orthotospoviruses (Zhang et al, 2011;Ali et al, 2013;van Vu, Choudhury & Mukherjee, 2013;Mitter et al, 2016). For example, transgenic tomato plants expressing amiRNAs targeting the transcripts of the pre-coat and coat proteins encoding sequences of tomato leaf curl New Delhi virus (ToLCNDV) showed tolerance to the virus infection (van Vu, Choudhury & Mukherjee, 2013).…”

Section: Introductionmentioning

confidence: 99%

Novel targets for engineering Physostegia chlorotic mottle and tomato brown rugose fruit virus-resistant tomatoes: in silico prediction of tomato microRNA targets

Gaafar

Ziebell

2020

PeerJ

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Background Physostegia chlorotic mottle virus (PhCMoV; genus: Alphanucleorhabdovirus, family: Rhabdoviridae) and tomato brown rugose fruit virus (ToBRFV; genus: Tobamovirus, family: Virgaviridae) are newly emerging plant viruses that have a dramatic effect on tomato production. Among various known virus-control strategies, RNAi-mediated defence has shown the potential to protect plants against various pathogens including viral infections. Micro(mi)RNAs play a major role in RNAi-mediated defence. Methods Using in silico analyses, we investigated the possibility of tomato-encoded miRNAs (TomiRNA) to target PhCMoV and ToBRFV genomes using five different algorithms, i.e., miRanda, RNAhybrid, RNA22, Tapirhybrid and psRNATarget. Results The results revealed that 14 loci on PhCMoV and 10 loci on ToBRFV can be targeted by the TomiRNAs based on the prediction of at least three algorithms. Interestingly, one TomiRNA, miR6026, can target open reading frames from both viruses, i.e., the phosphoprotein encoding gene of PhCMoV, and the two replicase components of ToBRFV. There are currently no commercially available PhCMoV- or ToBRFV-resistant tomato varieties, therefore the predicted data provide useful information for the development of PhCMoV- and ToBFRV-resistant tomato plants.

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Evaluation and identification of candidate genes for artificial microRNA-mediated resistance to tomato spotted wilt virus

Cited by 37 publications

References 54 publications

Multi‐targeting of viral RNAs with synthetic trans‐acting small interfering RNAs enhances plant antiviral resistance

Multi‐targeting of viral RNAs with synthetic trans‐acting small interfering RNAs enhances plant antiviral resistance

MicroRNA-Mediated Gene Silencing in Plant Defense and Viral Counter-Defense

Novel targets for engineering Physostegia chlorotic mottle and tomato brown rugose fruit virus-resistant tomatoes: in silico prediction of tomato microRNA targets

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