Evaluation and comparison of two improved techniques for the on-line detection of antioxidants in liquid chromatography eluates

Dapkevičius, A.; Beek, TA van; Niederländer, H.A.G.

doi:10.1016/s0021-9673(01)00548-9

Cited by 109 publications

(70 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The HPLC method is sensitive and can be used as a quality control tool for the rapid determination of free radical scavenging activity of variety of products including plant extracts, foods, drugs and polyherbal formulations. The applicability of HPLC method was extended to determine the antioxidant activity of crude plant extracts and drugs (Dapkevicius et al 1999;Karunakar et al 2003). The HPLC method developed was specific for DPPH with an acceptable reproducibility and short run time allowing for rapid determination of radical scavenging activity of several samples (Chandrasekar et al 2006).…”

Section: Developments In Dpph Methodsmentioning

confidence: 99%

“…The compounds of over a broad range of polarity and pKa can be evaluated which exhibit different kinetic behavior towards DPPH. It can employ both isocratic and gradient runs with mobile phases of different composition and pH can be carried out (Dapkevicius et al 1999). The major advantage of this method is that it is immediately clear which constituent possesses radical scavenging activity.…”

Section: Developments In Dpph Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Genesis and development of DPPH method of antioxidant assay

2011

View full text Add to dashboard Cite

α, α-diphenyl-β-picrylhydrazyl (DPPH) free radical scavenging method offers the first approach for evaluating the antioxidant potential of a compound, an extract or other biological sources. This is the simplest method, wherein the prospective compound or extract is mixed with DPPH solution and absorbance is recorded after a defined period. However, with the advancement and sophistication in instrumental techniques, the method has undergone various modifications to suit the requirements, even though the basic approach remains same in all of them. This article presents a critical review on various developments to the DPPH method.

show abstract

Section: Developments In Dpph Methodsmentioning

confidence: 99%

Section: Developments In Dpph Methodsmentioning

confidence: 99%

Genesis and development of DPPH method of antioxidant assay

2011

View full text Add to dashboard Cite

show abstract

“…A typical example developed in our group is online radical scavenging detection (Dapkevicius et al 1999;Koleva et al 2000;Koleva et al 2001;Dapkevicius et al 2001;Niederländer et al 2008). Phenolic antioxidants can terminate radical chain processes in lipid rich foods by reacting with reactive oxygen species (ROS) forming more stable resonance-stabilised radicals or quinone-like structures (Fig.…”

Section: High-resolution Screeningmentioning

confidence: 99%

Recent developments in the rapid analysis of plants and tracking their bioactive constituents

et al. 2009

Self Cite

View full text Add to dashboard Cite

show abstract

“…Some tests rely on photometric measurements of the concentration of artificial radicals, which absorb light at a suitable wavelength or react with additional chromophores [15]. Other methods use the ability of antioxidants to reduce luminol chemiluminescence [16,17]. The antioxidantmodulated damage inflicted on biomolecules by radicals can also serve as a test of the antioxidative properties [20,21].…”

Section: Introductionmentioning

confidence: 99%

Immobilized Cytochrome c Sensor in Organic/Aqueous Media for the Characterization of Hydrophilic and Hydrophobic Antioxidants

2003

View full text Add to dashboard Cite

A method for the characterization of antioxidants is introduced, which allows the measurement of pure hydrophilic and hydrophobic substances as well as complex cosmetic creams. The sensor is based on cytochrome c covalently immobilized on a gold wire electrode working in mixtures of phosphate buffer and organic solvents. It is combined with a superoxide generating enzyme system. The decrease of the superoxide concentration in the test solution by the added antioxidants is detected and used for the quantification of their antioxidative efficiency. Electrochemical properties of immobilized cytochrome c, such as formal potential and heterogeneous electron transfer rate constant, have been investigated in mixtures of aqueous buffer and DMSO, methanol, butanediol, and THF. The maximum organic solvent content for quasi-reversible electrode behavior was correlated to spectroscopic measurements. The activity of the radical producing enzyme in such media was determined and the radical generation characterized. The antioxidative properties of pure substance such as ascorbic acid and Biochanin A as well as of five anti-ageing cosmetic creams were studied. This showed also the influence of matrix composition on the efficiency of antioxidative supplements.

show abstract

Evaluation and comparison of two improved techniques for the on-line detection of antioxidants in liquid chromatography eluates

Cited by 109 publications

References 23 publications

Genesis and development of DPPH method of antioxidant assay

Genesis and development of DPPH method of antioxidant assay

Recent developments in the rapid analysis of plants and tracking their bioactive constituents

Immobilized Cytochrome c Sensor in Organic/Aqueous Media for the Characterization of Hydrophilic and Hydrophobic Antioxidants

Contact Info

Product

Resources

About