Evaluation and comparison of neutrophil bipolar shape formation with a migration assay.

Lord, RJ; Roath, S.

doi:10.1136/jcp.43.4.342

Cited by 11 publications

(3 citation statements)

References 10 publications

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“…Morphological examination of fixed neutrophils can be used to assess the proportion which are activated (i.e., the percentage with distorted instead of smooth spherical shapes). Cell shape can be quantitated using: (1) isolated neutrophils [66]; (2) stained blood films [60]; (3) whole blood fixed relatively mildly, followed by lysis of red cells [62]. The percentage of morphologically active cells has been found to correlate strongly with impaired filterability for purified cell suspensions [66].…”

Section: Formation Of Pseudopodia and F-actinmentioning

confidence: 99%

“…Whole blood can be used to measure leukocyte deformability in some filtration devices [29], for analysis of expression of adhesion receptors [13], measurement of cell-cell aggregation [80], and cell morphology with pseudopod formation [60]. Even if an activating stimulus may be present in vivo, the cells may be responding over time after withdrawal of whole blood.…”

Section: Precautions and Interpretation Of Datamentioning

confidence: 99%

See 1 more Smart Citation

New guidelines for hemorheological laboratory techniques

Başkurt¹,

Boynard²,

Cokelet

et al. 2009

Clinical Hemorheology and Microcirculation

414

267

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Section: Formation Of Pseudopodia and F-actinmentioning

confidence: 99%

Section: Precautions and Interpretation Of Datamentioning

confidence: 99%

New guidelines for hemorheological laboratory techniques

Başkurt¹,

Boynard²,

Cokelet

et al. 2009

Clinical Hemorheology and Microcirculation

414

267

View full text Add to dashboard Cite

“…While contact with a substratum is necessary for the locomotion of PMN, these cells polarise in suspension following the addition of chemotactic stimuli such as the synthetic peptide N-formyl-methionyl-leucylphenylalanine (FMLP) [18,27]. In general, the extent of polarisation displayed by PMN in suspension is directly proportional to their level of motility on substrata [18,22], but assays of the latter are influenced by the cells' degree of adhesion [1,2,28]. Assays of PMN polarisation in suspension have therefore been widely used as a rapid and simple alternative for assessing activation of the cell's internal motile apparatus [10,11,12,16,20,25].…”

Section: Introductionmentioning

confidence: 99%

Comparison of techniques for the assessment of polymorphonuclear leukocyte polarisation in suspension

Harkin¹,

Gadd²,

Bignold³

1993

Biology of the Cell

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Polarisation of polymorphonuclear leukocytes (PMN) in suspension was assessed using three techniques: 1) visual classification; 2) computerised morphometry; and 3) flow cytometry. While visual classification detected the formation, polarisation and type of cytoplasmic extensions produced by PMN, morphometry and flow cytometry detected only the formation of extensions. The area, perimeter and ellipticity were, in general, statistically different for each subtype of PMN-shape identified by visual classification. Furthermore, the magnitude and direction of changes detected by flow cytometry were affected by the use of erythrocyte lysis (during isolation of the cells) and the fixative used prior to analysis. The findings of this study demonstrate that visual classification is a more sensitive, reliable and appropriate assay of PMN polarisation than current morphometric and flow cytometric methods.

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Effect of aprotinin on neutrophil function after major vascular surgery

Lord

Roath

Thompson

et al. 1992

British Journal of Surgery

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High-dose aprotinin reduces blood loss and blood transfusion requirements during liver transplantation and cardiac and vascular surgery. The mechanism of the haemostatic effect of aprotinin is unclear. A general effect on the anti-inflammatory response may be involved. Because leucocyte activation is part of this process, white cell function was measured in patients undergoing aortic surgery who received high-dose aprotinin therapy (n = 10) and was compared with the results from controls who did not (n = 10). The test group received an intravenous bolus (2 x 10(6) kallikrein inhibitor units) of aprotinin after induction of anaesthesia followed by continuous infusion (0.5 x 10(6) kallikrein inhibitor units/h) until the end of the operation. Blood samples were obtained before operation, immediately after surgery, and 1 and 7 days after operation. Aprotinin maintained significantly better postoperative white cell function as measured by bipolar shape formation (P less than 0.001), unstimulated nitroblue tetrazolium (NBT) reduction (P less than 0.001) and chemotaxis (P less than 0.001). Endotoxin-stimulated NBT reduction was similar in both groups, indicating that neutrophils from treated individuals retained the capacity to respond to oxidative stimuli. Aortic surgery activates neutrophils in vivo, as reflected by impaired chemotaxis and increased superoxide production. Aprotinin protects the cells against this potentially deleterious effect without affecting their ability to respond when provoked. Whether this affects leucocyte interaction with coagulation pathways and contributes to the reduction in blood loss remains to be determined.

show abstract

Evaluation and comparison of neutrophil bipolar shape formation with a migration assay.

Cited by 11 publications

References 10 publications

New guidelines for hemorheological laboratory techniques

New guidelines for hemorheological laboratory techniques

Comparison of techniques for the assessment of polymorphonuclear leukocyte polarisation in suspension

Effect of aprotinin on neutrophil function after major vascular surgery

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