New guidelines for hemorheological laboratory techniques

Başkurt, Oğuz K.; Boynard, M.; Cokelet, Giles C.; Connes, Philippe; Cooke, Brian M.; Forconi, S; Liao, Fulong; Hardeman, Max R.; Jung, F.; Meiselman, Herbert J.; Nash, Gerard B.; Németh, Norbert; Neu, Björn; Sandhagen, Bo; Shin, Sehyun; Thurston, George B.; Jl, Wautier

doi:10.3233/ch-2009-1202

Cited by 414 publications

(283 citation statements)

References 68 publications

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“…Heparin was used as an anticoagulant; whole blood viscosity (including high\middle\low shear rates), hematocrit (HCT) and red blood cell sedimentation were checked [24] by an automatic blood rheometer (LBY-N6B, Beijing Precil Instrument Co. Ltd.).…”

Section: Methodsmentioning

confidence: 99%

Assessing Regional Cerebral Blood Flow in Depression Using 320-Slice Computed Tomography

et al. 2014

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While there is evidence that the development and course of major depressive disorder (MDD) symptomatology is associated with vascular disease, and that there are changes in energy utilization in the disorder, the extent to which cerebral blood flow is changed in this condition is not clear. This study utilized a novel imaging technique previously used in coronary and stroke patients, 320-slice Computed-Tomography (CT), to assess regional cerebral blood flow (rCBF) in those with MDD and examine the pattern of regional cerebral perfusion. Thirty nine participants with depressive symptoms (Hamilton Depression Rating Scale 24 (HAMD24) score >20, and Self-Rating Depression Scale (SDS) score >53) and 41 healthy volunteers were studied. For all subjects, 3 ml of venous blood was collected to assess hematological parameters. Trancranial Doppler (TCD) ultrasound was utilized to measure parameters of cerebral artery rCBFV and analyse the Pulsatility Index (PI). 16 subjects (8 = MDD; 8 = healthy) also had rCBF measured in different cerebral artery regions using 320-slice CT. Differences among groups were analyzed using ANOVA and Pearson's tests were employed in our statistical analyses. Compared with the control group, whole blood viscosity (including high\middle\low shear rate)and hematocrit (HCT) were significantly increased in the MDD group. PI values in different cerebral artery regions and parameters of rCBFV in the cerebral arteries were decreased in depressive participants, and there was a positive relationship between rCBFV and the corresponding vascular rCBF in both gray and white matter. rCBF of the left gray matter was lower than that of the right in MDD. Major depression is characterized by a wide range of CBF impairments and prominent changes in gray matter blood flow. 320-slice CT appears to be a valid and promising tool for measuring rCBF, and could thus be employed in psychiatric settings for biomarker and treatment response purposes.

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Section: Methodsmentioning

confidence: 99%

Assessing Regional Cerebral Blood Flow in Depression Using 320-Slice Computed Tomography

et al. 2014

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“…All hemorheological measurements were carried out within 4 hours of blood sampling to avoid blood rheological alterations [8], and after complete oxygenation of blood [9]. We strictly followed the international guidelines for the standardization in blood rheology techniques/measurements [9].…”

Section: Methodsmentioning

confidence: 99%

“…We strictly followed the international guidelines for the standardization in blood rheology techniques/measurements [9]. Blood viscosity was determined at native hematocrit, at 25 ° C and at 225 s − 1 using a cone/plate viscometer (Brookfield DVII+ with CPE40 spindle, Brookfield Engineering Labs, Natick, MA).…”

Section: Methodsmentioning

confidence: 99%

Effects of Hydration Level and Heat Stress on Thermoregulatory Responses, Hematological and Blood Rheological Properties in Growing Pigs

et al. 2014

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Heat stress is one of the major limiting factors of production efficiency in the swine industry. The aims of the present study were 1) to observe if hemorheological and hematological parameters could be associated to physiological acclimation during the first days of heat stress exposure and 2) to determine if water restriction could modulate the effect of thermal heat stress on physiological, hematological and hemorheological parameters. Twelve Large White male pigs were divided into an ad libitum and a water restricted group. All pigs were submitted to one week at 24°C (D-7 to D-1). Then, at D0, temperature was progressively increased until 32°C and maintained during one week (D1 to D7). We performed daily measurements of water and feed intake. Physiological (i.e., skin temperature, rectal temperature, respiratory rate), hematological and hemorheological parameters were measured on D-6, D-5, D0, D1, D2 and D7. Water restriction had no effect on physiological, hematological and hemorheological parameters. The first days of heat stress caused an increase in the three physiological parameters followed by a reduction of these parameters suggesting a successful acclimation of pigs to heat stress. We showed an increase in hematocrit, red blood cell aggregation and red blood cell aggregation strength during heat stress. Further, we observed an important release of reticulocytes, an increase of red blood cell deformability and a reduction of feed intake and blood viscosity under heat stress. This study suggests that physiological acute adaptation to heat stress is accompanied by large hematological and hemorheological changes.

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“…Although participants in both groups had free access to water in the postexercise period, maybe those consuming a meal were better hydrated. It has been recommended that the sampling should be done after overnight fasting for the determination of blood and plasma viscosity [24] but several postprandial studies reported a decrease in viscosity suggesting relative overnight dehydration [25, 26]. However in active healthy people moderate dehydration occurring during exercise has only a slight effect on blood viscosity [27, 28].…”

Section: Discussionmentioning

confidence: 99%

Effects of a Meal on the Hemorheologic Responses to Exercise in Young Males

Bilski

Teległów²,

Pokorski

et al. 2014

BioMed Research International

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Aim. This study investigates the changes in hemorheologic parameters resulting from exercise followed by a standard meal. Methods. In twelve moderately active men a period of exercise on a bicycle ergometer for 30 min at 60% VO2max was followed by a test meal or by 30 min rest. Venous blood was sampled for further analysis at baseline, after exercise, and after the meal/rest period. Results. The elongation index (EI) was reduced and a marked rise in plasma viscosity was observed after exercise. A significant decrease in half time of total aggregation (T 1/2) and a rise in aggregation index (AI) after exercise were observed; however, after the postexercise period these changes were reversed. Conclusion. The present study demonstrates that physical exercise causes several changes in blood rheology parameters, such as an increase of blood viscosity, a decrease in EI and an increase in AI, and a fall in the T 1/2 values. The meal eaten in the postexercise period caused a further reduction in EI values indicating higher red cell rigidity, but not in plasma viscosity or aggregations indices. Such alterations in hemorheologic parameters should not impair the function of the cardiovascular system in fit and healthy people but it could constitute a serious risk under various pathophysiological conditions.

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New guidelines for hemorheological laboratory techniques

Cited by 414 publications

References 68 publications

Assessing Regional Cerebral Blood Flow in Depression Using 320-Slice Computed Tomography

Assessing Regional Cerebral Blood Flow in Depression Using 320-Slice Computed Tomography

Effects of Hydration Level and Heat Stress on Thermoregulatory Responses, Hematological and Blood Rheological Properties in Growing Pigs

Effects of a Meal on the Hemorheologic Responses to Exercise in Young Males

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