Evaluating Tumor Angiogenesis

Abstract

The evaluation of tumor angiogenesis in pancreatic cancers involves determining the status of tumor vasculature and hypoxia in the tumor. Describing the nature and extent of tumor angiogenesis involves evaluating the expression of endothelial and perivascular cells within the tumor, and the expression of angiogenesis-related genes in tumor vasculature. Here we describe the methodology for assessment of tumor vasculature in murine mouse models of cancer. Specifically, we provide methodology for the evaluation of tumor hypoxia, tumor vessel perfusion, and chromogenic and fluorescent immunohistochemistry applied to tumor vascular analysis.

MicroRNAs-mediated regulation of glucose transporter (GLUT) expression in glioblastoma

“…At the same time, the ingrowing vessels have numerous defects: leaky post-capillary veins, weak blood flow due to the irregular course of the vessels, which poorly cope with providing the tumor with oxygen. If earlier it was believed that the main effect of hypoxia is the forced switching of the cell to a less favorable energy supply due to the impossibility of using a more profitable energy source - mitochondrial oxidation, now it is clear that hypoxia is a different state of the cell that supports and develops the tumor process [ 34 ]. Cells subjected to hypoxia show increased proliferation, arrest in differentiation by activating dedifferentiation genes (e.g.…”