Evaluating the uptake and intracellular fate of polystyrene nanoparticles by primary and hepatocyte cell lines in vitro

Johnston, Helinor Jane; Semmler‐Behnke, Manuela; Brown, David M.; Kreyling, Wolfgang G.; Tran, Lang; Stone, Vicki

doi:10.1016/j.taap.2009.09.015

Cited by 191 publications

(143 citation statements)

References 28 publications

Supporting

Mentioning

126

Contrasting

Unclassified

Order By: Relevance

“…These structures are responsible for the formation and secretion of bile by hepatocytes and could be indicative of NP removal from the cells. Interestingly, the elimination of polystyrene NPs (20 nm) in bile has been observed previously in vitro and in vivo [60].…”

Section: Discussionsupporting

confidence: 59%

“…Previous studies have indicated that the uptake of polymer NPs by cells increased with time in other cell lines (e.g. macrophages), although these studies did not look at the impact of NP concentration on uptake and assessed uptake over a shorter time frame [59,60]. Different cell types vary with respect to their efficiency at internalising NPs.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Synthesis, characterization and evaluation of in vitro toxicity in hepatocytes of linear polyesters with varied aromatic and aliphatic co-monomers

Kakde

Powell

Bansal

et al. 2016

Journal of Controlled Release

Self Cite

View full text Add to dashboard Cite

Section: Discussionsupporting

confidence: 59%

Section: Discussionmentioning

confidence: 99%

Synthesis, characterization and evaluation of in vitro toxicity in hepatocytes of linear polyesters with varied aromatic and aliphatic co-monomers

Kakde

Powell

Bansal

et al. 2016

Journal of Controlled Release

Self Cite

View full text Add to dashboard Cite

“…Furthermore, it has been presumed that the acidic nature of the cargo present in these vesicles, ie, carboxylated quantum dots, could contribute to acidification of these vesicles, which in turn targets them for strong staining with the reagent. Some cellular localization studies performed using nonacidic nanoparticles 45,46 showed that these nanoparticles did not colocalize with either early endosomes or lysosomes. Therefore, the effect of the quantum dot coating on intravesicular pH must be taken into account for interpretation of the results of intracellular localization.…”

Section: Discussionmentioning

confidence: 99%

Intracellular distribution of nontargeted quantum dots after natural uptake and microinjection

Damalakiene¹,

Karabanovas²,

Bagdonas³

et al. 2013

IJN

View full text Add to dashboard Cite

Background:The purpose of this study was to elucidate the mechanism of natural uptake of nonfunctionalized quantum dots in comparison with microinjected quantum dots by focusing on their time-dependent accumulation and intracellular localization in different cell lines. Methods:The accumulation dynamics of nontargeted CdSe/ZnS carboxyl-coated quantum dots (emission peak 625 nm) was analyzed in NIH3T3, MCF-7, and HepG2 cells by applying the methods of confocal and steady-state fluorescence spectroscopy. Intracellular colocalization of the quantum dots was investigated by staining with Lysotracker ® . Results:The uptake of quantum dots into cells was dramatically reduced at a low temperature (4°C), indicating that the process is energy-dependent. The uptake kinetics and imaging of intracellular localization of quantum dots revealed three accumulation stages of carboxyl-coated quantum dots at 37°C, ie, a plateau stage, growth stage, and a saturation stage, which comprised four morphological phases: adherence to the cell membrane; formation of granulated clusters spread throughout the cytoplasm; localization of granulated clusters in the perinuclear region; and formation of multivesicular body-like structures and their redistribution in the cytoplasm. Diverse quantum dots containing intracellular vesicles in the range of approximately 0.5-8 µm in diameter were observed in the cytoplasm, but none were found in the nucleus. Vesicles containing quantum dots formed multivesicular body-like structures in NIH3T3 cells after 24 hours of incubation, which were Lysotracker-negative in serum-free medium and Lysotracker-positive in complete medium. The microinjected quantum dots remained uniformly distributed in the cytosol for at least 24 hours. Conclusion: Natural uptake of quantum dots in cells occurs through three accumulation stages via a mechanism requiring energy. The sharp contrast of the intracellular distribution after microinjection of quantum dots in comparison with incubation as well as the limited transfer of quantum dots from vesicles into the cytosol and vice versa support the endocytotic origin of the natural uptake of quantum dots. Quantum dots with proteins adsorbed from the culture medium had a different fate in the final stage of accumulation from that of the protein-free quantum dots, implying different internalization pathways.

show abstract

“…Usually, it is regarded that membrane-wrapped NPs are trapped in intracellular vesicles such as early and late endosomes, lysosomes, or phagosomes. [20][21] Some studies also reported the presence of NPs in cytosol, 19,[22][23][24] in mitochondria, 15,25 and in nuclei. 23,26 The ways of entrance into cells and the subsequent distribution are crucial for their biological effects.…”

Section: Trafficking and Subcellular Distributionmentioning

confidence: 99%

“…23,26 The ways of entrance into cells and the subsequent distribution are crucial for their biological effects. 25,27 Shukla et al found that GNPs can be internalized in lysosomes and then the organelles move toward the nuclei with a peri-nucleu distribution in a time-dependent manner. 28 Our results demonstrated that intracellular localization is a key factor in determining final fates of both GNPs (retention or exclusion) and cells.…”

Section: Trafficking and Subcellular Distributionmentioning

confidence: 99%

Cellular Uptake, Intracellular Trafficking and Biological Responses of Gold Nanoparticles

Jiang

Wang

Chen

2011

J Chinese Chemical Soc

View full text Add to dashboard Cite

Promising application of gold nanoparticles (GNPs) in biomedical and environmental fields is increasing the likelihood of direct interaction with living systems. The knowledge about GNPs interacting with cells is quite limited and however, is necessary for risk prediction and evaluation of biological effects. Herein, we review recent advances in cellular effects of GNPs including cellular uptake, trafficking, subcellular distribution and cellular responses, and the relationship between physiochemical properties of GNPs and biological effects as well. To uncover these issues, it is quite instructive for safe and sustainable use of GNPs.

show abstract

Evaluating the uptake and intracellular fate of polystyrene nanoparticles by primary and hepatocyte cell lines in vitro

Cited by 191 publications

References 28 publications

Synthesis, characterization and evaluation of in vitro toxicity in hepatocytes of linear polyesters with varied aromatic and aliphatic co-monomers

Synthesis, characterization and evaluation of in vitro toxicity in hepatocytes of linear polyesters with varied aromatic and aliphatic co-monomers

Intracellular distribution of nontargeted quantum dots after natural uptake and microinjection

Cellular Uptake, Intracellular Trafficking and Biological Responses of Gold Nanoparticles

Contact Info

Product

Resources

About