Cellular Uptake, Intracellular Trafficking and Biological Responses of Gold Nanoparticles

Jiang, Xiumin; Wang, Liming; Chen, Chunying

doi:10.1002/jccs.201190024

Cited by 16 publications

(11 citation statements)

References 66 publications

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“…31 Adverse effects such as organelle disruption, membrane breakdown, and apoptosis can be induced. 30 However, the current study demonstrated no significant differences between ASCs loaded with GNTs compared to those without GNTs in respect to cell morphology, viability, or proliferation. In addition, both the current study and the authors' prior work using BMSCs demonstrate that exocytosis of GNTs does not contribute significantly to a signal loss relative to cellular proliferation.…”

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confidence: 63%

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Evaluation of gold nanotracers to track adipose-derived stem cells in a PEGylated fibrin gel for dermal tissue engineering applications

Chung¹,

Nam²,

Ricles³

et al. 2013

IJN

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Evaluating the regenerative capacity of a tissue-engineered device in a noninvasive and synchronous manner is critical to determining the mechanisms for success in clinical applications. In particular, directly tracking implanted cells in a three-dimensional (3D) scaffold is desirable in that it enables the monitoring of cellular activity in a specific and localized manner. The authors' group has previously demonstrated that the PEGylation of fibrin results in a 3D scaffold that supports morphologic and phenotypic changes in mesenchymal stem cells that may be advantageous in wound healing applications. Recently, the authors have evaluated adipose-derived stem cells (ASCs) as a mesenchymal cell source to regenerate skin and blood vessels due to their potential for proliferation, differentiation, and production of growth factors. However, tracking and monitoring ASCs in a 3D scaffold, such as a PEGylated fibrin gel, have not yet been fully investigated. In the current paper, nanoscale gold spheres (20 nm) as cell tracers for ASCs cultured in a PEGylated fibrin gel were evaluated. An advanced dual-imaging modality combining ultrasound and photoacoustic imaging was utilized to monitor rat ASCs over time. The ASCs took up gold nanotracers and could be detected up to day 16 with high sensitivity using photoacoustic imaging. There were no detrimental effects on ASC morphology, network formation, proliferation, and protein expression/secretion (ie, smooth muscle α-actin, vascular endothelial growth factor, matrix metalloproteinase-2, and matrix metalloproteinase-9) associated with gold nanotracers. Therefore, utilization of gold nanotracers can be an effective strategy to monitor the regenerative process of a stem cell source in a 3D gel for vascular and dermal tissue engineering applications.

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confidence: 63%

“…30 Compared to the absorbance of GNTs in solution, the absorbance of GNTs in cells showed red shifting and peak broadening ( Figure 2C and D). This is due to the aggregation-induced plasmon resonance coupling, a phenomenon which is well described in the literature.…”

mentioning

confidence: 91%

Evaluation of gold nanotracers to track adipose-derived stem cells in a PEGylated fibrin gel for dermal tissue engineering applications

Chung¹,

Nam²,

Ricles³

et al. 2013

IJN

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“…The surface of Au NPs is usually modified with electrolytes such as citrate, hexadecyl trimethyl ammonium bromide (CTAB), sodium polystyrene sulfonate (PSS), polyethylene diallyl dimethyl amine hydrochloride (PDDAC), etc in order to increase their dispersibility [78]. The surface modification provides enough net charges on the Au NPs, which may produce electrostatic attraction to the oppositely charged functional groups in proteins.…”

Section: Au Np–protein Interactionsmentioning

confidence: 99%

Interaction of gold nanoparticles with proteins and cells

Wang

et al. 2015

Science and Technology of Advanced Materials

164

117

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Gold nanoparticles (Au NPs) possess many advantages such as facile synthesis, controllable size and shape, good biocompatibility, and unique optical properties. Au NPs have been widely used in biomedical fields, such as hyperthermia, biocatalysis, imaging, and drug delivery. The broad application range may result in hazards to the environment and human health. Therefore, it is important to predict safety and evaluate therapeutic efficiency of Au NPs. It is necessary to establish proper approaches for the study of toxicity and biomedical effects. In this review, we first focus on the recent progress in biological effects of Au NPs at the molecular and cellular levels, and then introduce key techniques to study the interaction between Au NPs and proteins. Knowledge of the biomedical effects of Au NPs is significant for the rational design of functional nanomaterials and will help predict their safety and potential applications.

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“…This result indicates that optimal size and well-dispersed nanoparticles are important for photodynamic effect in aqueous media. Especially, bigger size (~60 nm) of nanoparticles 2a could be useful to transport more chlorine molecules into the cancer cells by endocytosis [28, 29, 40]. …”

Section: Resultsmentioning

confidence: 99%

Size-Dependent Photodynamic Activity of Gold Nanoparticles Conjugate of Water Soluble Purpurin-18-N-Methyl-D-Glucamine

Lkhagvadulam

Kim

Yoon

et al. 2013

BioMed Research International

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Gold nanoparticles (GNPs) conjugates of water soluble ionic photosensitizer (PS), purpurin-18-N-methyl-D-glucamine (Pu-18-NMGA), were synthesized using various molar ratios between HAuCl4 and Pu-18-NMGA without adding any particular reducing agents and surfactants. The PS-GNPs conjugates showed long wavelength absorption of range 702–762 nm, and their different shapes and diameters depend on the molar ratios used in the synthesis. In vitro anticancer efficacy of the PS-GNPs conjugates was investigated by MTT assay against A549 cells, resulting in higher photodynamic activity than that of the free Pu-18-NMGA. Among the PS-GNPs conjugates, the GNPs conjugate from the molar ratio of 1 : 2 (Au(III): Pu-18-NMGA) exhibits the highest photodynamic activity corresponding to bigger size (~60 nm) of the GNPs conjugate which could efficiently transport the PS into the cells than that of smaller size of the GNPs conjugate.

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Cellular Uptake, Intracellular Trafficking and Biological Responses of Gold Nanoparticles

Cited by 16 publications

References 66 publications

Evaluation of gold nanotracers to track adipose-derived stem cells in a PEGylated fibrin gel for dermal tissue engineering applications

Evaluation of gold nanotracers to track adipose-derived stem cells in a PEGylated fibrin gel for dermal tissue engineering applications

Interaction of gold nanoparticles with proteins and cells

Size-Dependent Photodynamic Activity of Gold Nanoparticles Conjugate of Water Soluble Purpurin-18-N-Methyl-D-Glucamine

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