Evaluating the toxicological effects of agrochemicals on glucocorticoid receptor and serum cortisol level in <i>Mozambique tilapia</i>

Pandya, Parth; Upadhyay, Alka; Thakkar, Bhumi; Parikh, Pragna

doi:10.1080/23312025.2018.1480338

Cited by 12 publications

(4 citation statements)

References 49 publications

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“…In addition, the half‐life of PE has been observed in the near‐aquatic environment by agricultural runoff (Singh & Singh, 2011). In line with this, previous studies performed by our group (Pandya & Parikh, 2016; Pandya et al., 2018; Upadhyay et al., 2014) have provided deeper insights into neuroendocrine toxicity at the expression level (gene and protein) and general biochemical alterations. Oreochromis mossambicus was selected as the test model due to its abundance in the freshwaters of India and its sturdiness (Moyle et al., 2010).…”

Section: Introductionsupporting

confidence: 74%

“…Male and female adult O. mossambicus were obtained from pure brooders with a length of 12 ± 3 cm and weight of 25 ± 3 g. Fish (five males and five females) were acclimated in a clean glass tank for 15 days in de‐chlorinated water at 27 ± 4°C, pH 7.4 ± 0.05, dissolved oxygen 8 ± 0.3 mg/L and total hardness 188 mg/L CaCO 3 with a 12:12 light: dark photoperiod according to Pandya et al. (2018). If in any batch mortality exceeded 5% during acclimatisation, that entire batch was discarded.…”

Section: Methodsmentioning

confidence: 99%

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Herbicide pyrazosulfuron ethyl induces genotoxicity, cell cycle alterations and apoptosis in tilapia (Oreochromis mossambicus)

Upadhyay

Pandya²,

Salunke

et al. 2022

Aquaculture Fish & Fisheries

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Fishes are excellent subjects for the study of the mutagenic and carcinogenic potential of contaminants present in water. Herbicides are one of the toxic contaminants that cause lethal effects in fish. In this context, the present study was designed to study the sub‐lethal toxic effect (low dose [LD] 25 mg/L, medium dose‐50 mg/L, and high dose [HD] 100 mg/L) of a commonly used herbicide, pyrazosulfuron ethyl (PE), on erythrocytes of tilapia. The genotoxic effect was studied to understand the sub‐lethal potential by means of (a) the nuclear abnormalities, including the micronucleus (MN) frequency; (b) further, each stage of the cell cycle was analysed using flow cytometry and (c) finally, the targets of PE was deciphered using in silico analysis. The results showed that there were significant effects of time (7 and 14 days) and doses in the increase in the frequency of MN in erythrocytes of fish treated with PE, compared to the control group. After exposing fish to PE for 7 and 14 days, there was a proportional increase in the frequency of the apoptotic population of erythrocytes for LD to HD. During both time exposures (7 and 14 days), the highest cell death was accounted for in HD. Moreover, the majority of the cells showed a dysregulated cell cycle in the G1 phase in all the treated groups of PE, compared to the control group. This study showed that PE might initiate genotoxicity in non‐target species (tilapia) of the aquatic ecosystem near agricultural fields.

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Section: Introductionsupporting

confidence: 74%

Section: Methodsmentioning

confidence: 99%

Herbicide pyrazosulfuron ethyl induces genotoxicity, cell cycle alterations and apoptosis in tilapia (Oreochromis mossambicus)

Upadhyay

Pandya²,

Salunke

et al. 2022

Aquaculture Fish & Fisheries

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“…The fishes were sacrificed by decapitation on the 15 th day and tissues of thyroid follicles were dissected out from the lower pharyngeal portion after the oozing of the blood. Total RNA isolation was performed using Trizol (Invitrogen) according to the method of Pandya, Upadhyay, Thakkar, and Parikh () and concentration was measured spectroscopically by Perkin Elmer. Thermo Verso cDNA kit (AB‐1453/B, USA) was employed to reverse‐transcribed cDNA from 50ng isolated RNA.…”

Section: Methodsmentioning

confidence: 99%

“…Trizol (Invitrogen) according to the method ofPandya, Upadhyay, Thakkar, and Parikh (2018) and concentration was measured spectroscopically by…”

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confidence: 99%

Evaluating the toxic potential of agrochemicals on the hypothalamic‐pituitary‐thyroid axis in tilapia(Oreochromis mossambicus)

2020

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Agrochemicals are a major cause of concern for the aquatic environment because of their toxicity, persistence, and tendency to accumulate in the organisms. The impact of these chemicals on aquatic organisms is due to their movement from various diffuse or point sources, which poses a great threat to aquatic fauna especially fishes, which constitute one of the major sources of protein‐rich food for mankind. The present study is a first of its kind, where the toxic potential of two sublethal concentration (LC1/10th and LC1/25th) of four different classes of agrochemicals have been tested (Insecticide‐ Imidacloprid‐0.074 ppm, 0.02 ppm, Fungicide‐Curzate‐ 4.9 ppm, 1.96 ppm, Herbicide‐ Pyrazosulphuron ethyl‐50 ppm, 20 ppm and Fertilizer‐Micronutrient mixture 500 ppm, 200 ppm) on candidate markers of hypothalamus pituitary‐thyroid axis (TSH, T3, T4, TSHβr) in Oreochromis mossambicus (tilapia) by validating hormonal level and mRNA expression. The results reveal that exposure to agrochemicals resulted in a broad range of alterations with maximum damage being caused by insecticide followed by herbicide and fungicide in that order on the thyroid axis. The results of the present study highlight the need for more detailed studies on the effects of agrochemicals that accumulate in organisms and propose that there should be a check on the rampant use of agrochemicals.

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Hexaflumuron insecticide exposure induces behavior alterations, hemato-biochemical disorders, antioxidant-immune dysfunction, and histopathological alterations in Nile tilapia (Oreochromis niloticus)

Ibrahim,

Fouda,

Abdelwarith

et al. 2024

Vet Res Commun

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Evaluating the toxicological effects of agrochemicals on glucocorticoid receptor and serum cortisol level in Mozambique tilapia

Cited by 12 publications

References 49 publications

Herbicide pyrazosulfuron ethyl induces genotoxicity, cell cycle alterations and apoptosis in tilapia (Oreochromis mossambicus)

Herbicide pyrazosulfuron ethyl induces genotoxicity, cell cycle alterations and apoptosis in tilapia (Oreochromis mossambicus)

Evaluating the toxic potential of agrochemicals on the hypothalamic‐pituitary‐thyroid axis in tilapia(Oreochromis mossambicus)

Hexaflumuron insecticide exposure induces behavior alterations, hemato-biochemical disorders, antioxidant-immune dysfunction, and histopathological alterations in Nile tilapia (Oreochromis niloticus)

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