Evaluating the toxic potential of agrochemicals on the hypothalamic‐pituitary‐thyroid axis in tilapia(Oreochromis mossambicus)

Abstract

Agrochemicals are a major cause of concern for the aquatic environment because of their toxicity, persistence, and tendency to accumulate in the organisms. The impact of these chemicals on aquatic organisms is due to their movement from various diffuse or point sources, which poses a great threat to aquatic fauna especially fishes, which constitute one of the major sources of protein‐rich food for mankind. The present study is a first of its kind, where the toxic potential of two sublethal concentration (LC1/10th and LC1/25th) of four different classes of agrochemicals have been tested (Insecticide‐ Imidacloprid‐0.074 ppm, 0.02 ppm, Fungicide‐Curzate‐ 4.9 ppm, 1.96 ppm, Herbicide‐ Pyrazosulphuron ethyl‐50 ppm, 20 ppm and Fertilizer‐Micronutrient mixture 500 ppm, 200 ppm) on candidate markers of hypothalamus pituitary‐thyroid axis (TSH, T3, T4, TSHβr) in Oreochromis mossambicus (tilapia) by validating hormonal level and mRNA expression. The results reveal that exposure to agrochemicals resulted in a broad range of alterations with maximum damage being caused by insecticide followed by herbicide and fungicide in that order on the thyroid axis. The results of the present study highlight the need for more detailed studies on the effects of agrochemicals that accumulate in organisms and propose that there should be a check on the rampant use of agrochemicals.

Herbicide pyrazosulfuron ethyl induces genotoxicity, cell cycle alterations and apoptosis in tilapia (Oreochromis mossambicus)

“…As the maximum change observed was in MD and HD, we also predicted that the variations occurring in the gene expression pattern of these proteins would also be significant at both doses. This is because of our previously published data (Pandya et al, 2020), where we have shown a strong link between agrochemicals and endocrine markers, that is, hormones and their receptors are possible targets for agrochemicals, resulting in their multitarget competence. Nevertheless, the molecular structure analysis of PE through in silico applications and Gas Chromatography and Mass Spectrometry (GC-MS) studies will add to the present knowledge.…”

Decoding the effect of photoperiodic cues in transducing kisspeptin‐melatonin circuit during the pubertal onset in common carp

“…Quantitative real‐time PCR was executed using SYBR Green master‐mix (ThermoFisher: A25742) for 40 cycles, following the protocol by Pandya et al, 2020. The “Ct value” was employed to calculate the relative expression of the gene of interest using Microsoft Excel, and graphical representations were generated using GraphPad Prism software.…”