Evaluating the sterility of orthodontic materials as received from the manufacturer and that exposed to clinic environment - in vitro study

Abstract

Introduction: Sterilization in orthodontics has been discussed and stressed over times in the dental literature. Sterility of orthodontic materials should be of prime interest to the orthodontist as a health care professional and he should take appropriate measures to prevent the risk of cross infection.Aim: To evaluate the sterility of orthodontic materials as received from the manufacturer and that exposed to clinic environment for 6 months. Materials and methods:The sterility of orthodontic materials "as received" from the manufacturer and "clinically exposed" (unused materials stored in the clinic environment for a period of 6 months) was microbiologically evaluated (universal & conventional PCR).The materials which were included in this study were the sealed and unsealed orthodontic archwires, molar bands, elastomeric module, stainless steel bracket, coil spring and tungsten carbide burs of various brands (American Orthodontics, 3M Unitek, Ormco, Orthosystems, G&H, Modern Orthodontics, SS white etc).Results: After universal and conventional PCR, the detection of micro-organisms in all materials was significantly higher in the clinically exposed samples compared to as received samples from manufacturers (P <0.001). The detection of micro-organisms in all orthodontic wires is significantly higher in the unsealed samples compared to sealed samples (P <0.001). In universal PCR, the detection of microorganisms in all materials (Coil spring, E-chain, Elastomeric, Molar brands, Tungsten carbide) except for SS brackets is significantly higher in both as received and clinically exposed samples. Conclusion:After universal and conventional PCR, we conclude that bacteria were present on almost all orthodontic materials "as received from the manufacturers" and that exposed to a clinical environment. Therefore all materials should be sterilized before use in patients. 13 South Eur J Orthod Dentofac Res Musaddique SS. et al. Evaluating the sterility of orthodontic materials

Comparative Evaluation of Antibacterial Activity of Chlorhexidine, Povidone Iodine and Glutaraldehyde for Disinfection of Orthodontic Appliances - An In-Vitro Study

“…Previous studies have reported that all the sealed and unsealed brackets, archwires and molar bands, received directly from the manufacturers were contaminated with various organisms. [16,17] According to Lucas et al, both aerobic and anaerobic bacteria can produce bacteremia as a result of orthodontic treatment. [18] In vivo studies comparing the effects of Povidone iodine gargle and Chlorhexidine gluconate mouthwash on six different bacterial strains showed that Povidone iodine reduced the viable cell count.…”

Orthodontics - during and after COVID-19 pandemic

“…Individually sealed autoclavable arch wire packages should be preferred over unsealed ones for patient safety. 38 3. Arch wires should be ligated with stainless steel ligatures instead of elastomeric rings as the latter causes comparatively more plaque retention and gingival bleeding.…”