Evaluating the efficacy of turbimetric measurements as a rapid screening technique to assess fungal susceptibility to antimicrobial compounds as exemplified by the use of sodium metabisulfite

Natskoulis, Pantelis I.; Lappa, Iliada K.; Panagou, Efstathios Ζ.

doi:10.1016/j.foodres.2018.01.058

Cited by 15 publications

(8 citation statements)

References 21 publications

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“…Since sodium metabisulphite is an antimicrobial agent (Natskoulis et al . ), it's likely that the apparent increase in DSV was due to the antimicrobial properties of this additive. Our results also indicated that sodium metabisulphite (1 g l −1 ) may not affect the growth of DSV.…”

Section: Resultsmentioning

confidence: 99%

“…In the single factor experiment, the individual addition of sodium metabisulphite (1 g l À1 ) significantly increased the relative abundance of DSV. Since sodium metabisulphite is an antimicrobial agent (Natskoulis et al 2018), it's likely that the apparent increase in DSV was due to the antimicrobial properties of this additive. Our results also indicated that sodium metabisulphite (1 g l À1 ) may not affect the growth of DSV.…”

Section: Resultsmentioning

confidence: 99%

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Isolation of Desulfovibrio spp. from human gut microbiota using a next‐generation sequencing directed culture method

Chen

Zhou

Fang

et al. 2019

Lett Appl Microbiol

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Desulfovibrio spp. is predominant member of sulphate‐reducing bacteria in human gut microbiota. Previous studies indicated that the isolation of Desulfovibrio strains from human faecal samples is very important to study the roles of human intestinal Desulfovibrio spp. in maintaining healthy states or causing diseases, as well as defining their biological characteristics. However, there are very few reports describing the isolation of Desulfovibrio spp. from human faecal samples. In this study, faecal samples were inoculated into various media containing different components. The enriched culture communities were identified using 16S rRNA gene high‐throughput sequencing analysis, enabling us to identify the specific components that enable the enrichment of Desulfovibrio. Using this information, we developed five specific media and identified an effective enrichment medium that produced the highest relative abundance of Desulfovibrio in communities cultured from four faecal samples (26·5, 73·5, 44·7 and 77·6% respectively). In addition, the major non‐Desulfovibrio genera were identified. Finally, three species of Desulfovibrio, D. desulfuricans, D. piger and D. legallii were isolated, representing the first time that has D. legallii been isolated from a human gastrointestinal source. Significance and Impact of the Study ost of the human intestinal bacteria have not been cultured because of lack of appropriate culture method and appropriate media. Desulfovibrio spp. is associated with several clinical conditions like inflammatory bowel disease, but until now there are very few reports describing the isolation of Desulfovibrio spp. from human faecal samples. In this study, 16S rRNA gene high‐throughput sequencing analysis was applied to screen appropriate enrichment media and selective cultivation of Desulfovibrio. This sequencing‐based directed culture method described here can be used for the selective cultivation of gut bacteria of interest.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Isolation of Desulfovibrio spp. from human gut microbiota using a next‐generation sequencing directed culture method

Chen

Zhou

Fang

et al. 2019

Lett Appl Microbiol

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“…Spectrophotometric assays have been used to develop turbidimetric growth curves for a wide number of yeast and moulds (Meletiadis et al, 2001, 2003; Natskoulis, Lappa, & Panagou, 2018), and are found to be in agreement with standards of the Clinical and Laboratory Standards Institute (CLSI, 2008). Spectrophotometric assays have now been adopted to test antimicrobial susceptibility in both human and veterinary medicine as they are regarded as highly reproducible between laboratories and users.…”

Section: Discussionmentioning

confidence: 91%

In vitro susceptibility testing for the emerging pathogenic mould Veronaea botryosa and pharmacokinetic parameters of natamycin in white sturgeon (Acipenser transmontanus)

Coleman

Martínez‐López

Knych

et al. 2022

Journal of Fish Diseases

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Systemic phaeohyphomycosis caused by the dematiaceous mould Veronaea botryosa is an important emergent disease affecting captive sturgeons (Acipenser spp.). The disease, colloquially known as “fluid belly,” causes morbidity and mortality in adult animals resulting in significant economic losses to the aquaculture industry. Advancements in therapeutic and prophylactic protocols have been partially hampered by the lack of basic protocols to grow and manipulate the fungus in the laboratory. In this study, microbroth kinetic protocols were established to analyse V. botryosa growth in seven nutrient media at different temperatures. Generated area under the curve (AUC) indicates that potato flake dextrose broth (PFD‐B) and Sabouraud dextrose broth (SD‐B) incubated at 25°C provided the greatest growth. The generated protocol was then used to test the susceptibility of V. botryosa isolates to natamycin, a macrolide polyene antifungal agent used as a food preservative. SD‐B and RPMI with l‐glutamine (+RPMI‐B) containing different concentrations of natamycin were inoculated with V. botryosa conidia and the generated growth curves were compared using cubic smoothing spline model. The non‐inhibitory concentration and minimal inhibitory concentration (MIC; decrease of AUC by 90% compared with control) were determined to be <1 μg/mL and 16 μg/mL of natamycin in SD‐B media. To gain an understanding of the tissue distribution of natamycin in white sturgeon, pharmacokinetics was tested. Based on pharmacokinetic parameters determined in this study and targeting a blood concentration >16 μg/mL for 24 h, an intravenous dose >1 g/kg would be needed, making the use of this drug unrealistic. The information presented in this study can be used to investigate susceptibility of pathogenic fungus to antimicrobials and disinfectants as well as support future therapeutic protocols against emerging fungal diseases like fluid belly.

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“…MBS is an additive used mainly for its antimicrobial action and has been strongly related to damage to the cardiovascular system (Natskoulis et al, 2018;Noorafshan et al, 2014). Noorafshan et al (2014) carried out a study to evaluate the effects of MBS on the cardiovascular system of rats for an eight-week period.…”

Section: Synthetic Preservatives In Meat and Meat Productsmentioning

confidence: 99%

Natural compounds obtained from plants as alternatives for meat and meat products preservation

Lacerda

Paulino

Esteves

et al. 2021

RSD

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The purpose of this review is to demonstrate the findings on the use of natural compounds as preservatives in meat and meat products as a healthier and safer alternative compared to the use of synthetic preservatives. Due to its biological composition, meat and its derivatives are susceptible to spoilage, a process that can be delayed with the use of synthetic food preservatives. However, it is known that some products can trigger undesirable effects on consumer health. In recent years, there has been a greater demand for products obtained naturally or directly from a biological organism without laboratory interference in their composition, in order to reduce risks to the consumer. The most promising compounds belong to the groups of phenolic and flavonoid compounds, which have a proven, positive effect on oxidation and microbial development. Findings on the use of natural compounds as preservatives in meat and meat products demonstrate an alternative to increased shelf life without risking consumer health.

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Evaluating the efficacy of turbimetric measurements as a rapid screening technique to assess fungal susceptibility to antimicrobial compounds as exemplified by the use of sodium metabisulfite

Cited by 15 publications

References 21 publications

Isolation of Desulfovibrio spp. from human gut microbiota using a next‐generation sequencing directed culture method

Isolation of Desulfovibrio spp. from human gut microbiota using a next‐generation sequencing directed culture method

In vitro susceptibility testing for the emerging pathogenic mould Veronaea botryosa and pharmacokinetic parameters of natamycin in white sturgeon (Acipenser transmontanus)

Natural compounds obtained from plants as alternatives for meat and meat products preservation

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