Evaluating the effects of extended cold ischemia on interstitial metabolite in grafts in kidney transplantation using microdialysis

Fonouni, Hamidreza; Jarahian, Parvin; Rad, Morva Tahmasbi; Golriz, Mohammad; Faridar, Alireza; Esmaeilzadeh, Majid; Hafezi, Mohammadreza; Macher-Goeppinger, Stephan; Longerich, Thomas; Orakcioglu, Berk; Sakowitz, Oliver W.; Garoussi, Camelia; Mehrabi, Arianeb

doi:10.1007/s00423-012-1010-0

Cited by 7 publications

(3 citation statements)

References 52 publications

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“…This is probably because there is still some glucose delivery to the kidney from the arterial supply through the first two interventions. Traditionally, glycerol has been viewed as one of the first markers for ischemia and cell damage[10,14,29]. However, this is not in line with our results which show glycerol and glucose as slow markers first giving a warning after the last venous intervention and then lactate and glutamate as fast markers, increasing after the first intervention.…”

Section: Discussioncontrasting

confidence: 95%

The unsuitability of implantable Doppler probes for the early detection of renal vascular complications – a porcine model for prevention of renal transplant loss

Amdisen¹,

Jespersen²,

Møldrup³

et al. 2017

PLoS ONE

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BackgroundVascular occlusion is a rare, but serious complication after kidney transplantation often resulting in graft loss. We therefore aimed to develop an experimental porcine model for stepwise reduction of the renal venous blood flow and to compare an implantable Doppler probe and microdialysis for fast detection of vascular occlusion.MethodsIn 20 pigs, implantable Doppler probes were placed on the renal artery and vein and a microdialysis catheter was placed in the renal cortex. An arterial flowprobe served as gold standard. Following two-hour baseline measurements, the pigs were randomised to stepwise venous occlusion, complete venous occlusion, complete arterial occlusion or controls.ResultsAll parameters were stable through baseline measurements. Glutamate and lactate measured by microdialysis increased significantly (p = 0.02 and p = 0.03 respectively) 30 minutes after a 2/3 (66%) reduction in renal blood flow. The implantable Doppler probe was not able to detect flow changes until there was total venous occlusion. Microdialysis detected changes in local metabolism after both arterial and venous occlusion; the implantable Doppler probe could only detect vascular occlusions on the vessel it was placed.ConclusionsWe developed a new model for stepwise renal venous blood flow occlusion. Furthermore, the first comparison of the implantable Doppler probe and microdialysis for detection of renal vascular occlusions was made. The implantable Doppler probe could only detect flow changes after a complete occlusion, whereas microdialysis detected changes earlier, and could detect both arterial and venous occlusion. Based on these results, the implantable Doppler probe for early detection of vascular occlusions cannot be recommended.

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Section: Discussioncontrasting

confidence: 95%

The unsuitability of implantable Doppler probes for the early detection of renal vascular complications – a porcine model for prevention of renal transplant loss

Amdisen¹,

Jespersen²,

Møldrup³

et al. 2017

PLoS ONE

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“…There Kidney transplantation. Generally, experimental studies on a transplanted kidney model aim at revealing the relations between the warm ischemia time and cold ischemia time and glucose interstitial metabolism both: at preservation and after reperfusion [32,34,36,39,40]. No blood flow in an organ is typically due to depletion of glucose and pyruvate supplies in addition to a growing concentration of lactate and glycerol.…”

Section: The Findings Of Experimental and Clinical Studies On Using Mmentioning

confidence: 99%

“…Experimental study Clinical study Liver n=6 [16][17][18][19][20]22] n=10 [21,[23][24][25][26][27][28][29][30][31] Kidney n=11 [32][33][34][35][36][37][38][39][40][41][42] n=1 [43] N o t e: n -number of studies; literature references are given in square brackets.…”

Section: Organsmentioning

confidence: 99%

Microdialysis: Opportunities and Prospects in Liver Transplantation (Review)

Сушков¹,

Voskanyan²,

Губарев³

2018

Sovrem Tehnol Med

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The assessment of donor organ quality and the initial graft function is of crucial importance, especially in liver transplantation. Recent studies have shown the feasibility of microdialysis to monitor energy metabolism in renal and liver grafts. This method is powerful and suitable to study chemical composition of interstitial fluid. It is possible to identify organ-related changes that occur early and are not reflected by changes in plasma. Currently, microdialysis is primarily applied in neurology and neurosurgery to assess the volume and severity of the brain tissue injury. The use of the technique to study the mechanisms of ischemia-reperfusion graft injury and the processes of early allograft dysfunction is a promising research trend. The review presents the findings of both experimental and clinical trials, and discusses the advisability and prospects of microdialysis monitoring in liver transplantation.

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Microdialysis Monitoring

Córcoles

Boutelle

2013

Biosensors and Invasive Monitoring in Clinical Applications

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Evaluating the effects of extended cold ischemia on interstitial metabolite in grafts in kidney transplantation using microdialysis

Cited by 7 publications

References 52 publications

The unsuitability of implantable Doppler probes for the early detection of renal vascular complications – a porcine model for prevention of renal transplant loss

The unsuitability of implantable Doppler probes for the early detection of renal vascular complications – a porcine model for prevention of renal transplant loss

Microdialysis: Opportunities and Prospects in Liver Transplantation (Review)

Microdialysis Monitoring

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