Evaluating specific adhesion of Plasmodium falciparum-infected erythrocytes to immobilised hyaluronic acid with comparison to binding of mammalian cells

Beeson, James G.; Rogerson, Stephen J.; Brown, Graham V.

doi:10.1016/s0020-7519(02)00097-8

Cited by 19 publications

(17 citation statements)

References 36 publications

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“…falciparum adhesion assays. Adhesion assays were performed as previously described (42,66) using gelatin-enriched P. falciparum trophozoite-IEs at 15%-20% parasitemia. Incubations were conducted at 37°C for 30 minutes, and wash steps were performed with plain RPMI-HEPES.…”

Section: Methodsmentioning

confidence: 99%

Targets of antibodies against Plasmodium falciparum–infected erythrocytes in malaria immunity

et al. 2012

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Plasmodium falciparum is the major cause of malaria globally and is transmitted by mosquitoes. During parasitic development, P. falciparum-infected erythrocytes (P. falciparum-IEs) express multiple polymorphic proteins known as variant surface antigens (VSAs), including the P. falciparum erythrocyte membrane protein 1 (PfEMP1). VSA-specific antibodies are associated with protection from symptomatic and severe malaria. However, the importance of the different VSA targets of immunity to malaria remains unclear, which has impeded an understanding of malaria immunity and vaccine development. In this study, we developed assays using transgenic P. falciparum with modified PfEMP1 expression to quantify serum antibodies to VSAs among individuals exposed to malaria. We found that the majority of the human antibody response to the IE targets PfEMP1. Furthermore, our longitudinal studies showed that individuals with PfEMP1-specific antibodies had a significantly reduced risk of developing symptomatic malaria, whereas antibodies to other surface antigens were not associated with protective immunity. Using assays that measure antibody-mediated phagocytosis of IEs, an important mechanism in parasite clearance, we identified PfEMP1 as the major target of these functional antibodies. Taken together, these data demonstrate that PfEMP1 is a key target of humoral immunity. These findings advance our understanding of the targets and mediators of human immunity to malaria and have major implications for malaria vaccine development.

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Section: Methodsmentioning

confidence: 99%

Targets of antibodies against Plasmodium falciparum–infected erythrocytes in malaria immunity

et al. 2012

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“…For P. falciparum, such coincidence among the reduction of peripheral parasitaemia and the completion of placental development led to hypothesize that placental cytoadherence of parasites is the explanation for this observation [38,39]. After delivery, a rapid clearance of parasitaemia is observed [40], although in some cases it might persist [36]. As already mentioned, placental parasitaemia is rarely explored, particularly at early stages, and in regions where P. falciparum is predominant, term pregnancies exhibit higher parasite densities than peripheral blood [37,41].…”

Section: Dynamics Of Gestational and Placental Malariamentioning

confidence: 99%

Immune responses during gestational malaria: a review of the current knowledge and future trend of research

Maestre

Carmona‐Fonseca

2014

J Infect Dev Ctries

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Women pregnant with their first child are susceptible to severe P. falciparum disease from placental malaria because they lack immunity to placenta-specific cytoadherence proteins. In subsequent pregnancies, as immunity against placental parasites is acquired, there is a reduced risk of adverse effects of malaria on the mother and fetus and asymptomatic parasitaemia is common. In the case of vivax malaria, with increasing reports of severe cases in Asia and South America, the effects of infection by this species during pregnancy remain to be elucidated. This review summarized the main aspects involved in the acquisition of specific antimalarial immune responses during pregnancy with emphasis in research carried out in America and Asia, in order to offer a framework of interpretation for studies on pregnant women with malaria which are recently being produced in these regions. The authors conclude that (1) Effective humoral responses during gestational malaria are mainly directed against variant surface antigens codified by genes of the var2Csa family of P. falciparum; (2) Acquisition of immunity against these variant antigens depends on the degree and intensity of transmission, and the chance increases with age and successive pregnancies; (3) Antibody development is guided by specific cellular immune responses in cases of placental and maternal infection, and (4) The study of the significance of acquisition of specific immunity against both P. falciparum and P. vivax in America, should be performed.

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“…10 IE binding to HA seemed to be weaker than to CSA, less common among placental IEs, and less PAM-specific than adhesion to CSA, and the relative importance of CSA and HA as placental adhesion receptors for IEs has been the subject of some controversy. Since the original report, various studies have reported that IEs can bind to HA independently of their affinity for CSA 10,11 or have dismissed binding to HA as the result of contaminating CSA in the HA preparations used.…”

Section: Chondroitin Sulfate a And Hyaluronic Acid As Infected Erythrmentioning

confidence: 99%

Adhesion Specificities of Plasmodium falciparum-Infected Erythrocytes Involved in the Pathogenesis of Pregnancy-Associated Malaria

Hviid

2007

The American Journal of Pathology

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Evaluating specific adhesion of Plasmodium falciparum-infected erythrocytes to immobilised hyaluronic acid with comparison to binding of mammalian cells

Cited by 19 publications

References 36 publications

Targets of antibodies against Plasmodium falciparum–infected erythrocytes in malaria immunity

Targets of antibodies against Plasmodium falciparum–infected erythrocytes in malaria immunity

Immune responses during gestational malaria: a review of the current knowledge and future trend of research

Adhesion Specificities of Plasmodium falciparum-Infected Erythrocytes Involved in the Pathogenesis of Pregnancy-Associated Malaria

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