Evaluating of Induction of Apoptosis by Cornus mass L. Extract in the Gastric Carcinoma Cell Line (AGS)

Hosseini, Farzaneh Sadat; Karimabad, Mojgan Noroozi; Hajizadeh, Mohammad Reza; Khoshdel, Alireza; Falahati-Pour, Soudeh Khanamani; Mirzaei, Mohammad Reza; Mirmohamadi, Seyed Mehdi; Mahmoodi, Mehdi

doi:10.31557/apjcp.2019.20.1.123

Cited by 12 publications

(12 citation statements)

References 45 publications

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“…The effect of BAE was evaluated on MC38 tumour cells and normal cells. As L929 cells are frequently used as normal control cells to detect apoptotic effects [30][31][32], we used L929 cells as the model of normal cells. According to the data in the literature [2,33,34] and the experimental results of IC50 (Table S2), four doses (100, 200, 500 and 1000 μM) of ANC were used for the MTT assays.…”

Section: Inhibitory Effects Of the Bilberry Anthocyanins Extract (Bae)mentioning

confidence: 99%

Glycosylation of anthocyanins enhances the apoptosis of colon cancer cells by handicapping energy metabolism

Jing

Song

Liu

et al. 2020

BMC Complement Med Ther

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Background While anthocyanins are proven to be effective in inhibiting tumour cell proliferation, the underlying mechanisms remain unclear. This research aims to explore the glycosylation of anthocyanins in the tumour inhibitory effects and the potential mechanism. Methods The tumour inhibitory effect on mouse colon cancer cells (MC38) was examined by MTT and flow cytometric analyses. The inhibitory pathway of anthocyanin was explored by assessment of tumour cell mitochondrial membrane potential (MMP), the caspase-3 and caspase-9 activity, as well as the cell energy metabolism in terms of the glucose uptake, the NAD+/NADH ratio and the ATP level. Results We found that 500 μM bilberry anthocyanins extract (BAE) induced 48.1% mitochondrial damage, activated the downstream caspase cascade to form apoptotic bodies (caspase-3 activity increased by 169%, caspase-9 activity increased by 186%), and inhibited cell proliferation (survival rate: 55.97%, 24 h). In contrast, the same concentration of anthocyanidin (cyanidin) led to marginal mitochondrial damage (only 9.85%) and resulted in little inhibition of MC38 cells (survival rate: 86.84%, 24 h). For cells incubated with 500 μM BAE, reactive oxygen species (ROS) decreased by 53.8%, but the ratio of NAD+/NADH increased to 3.67, demonstrating that the mitochondrial damage was induced by blocking energy metabolism. Furthermore, cell energy metabolism is related to glucose uptake since the presence of 200 μM GLUT1 inhibitor substantially enhanced the inhibitory effects of cyanidin-3-O-glucoside (Cy-3-Glu) at 500 μM (survival rate: 51.08%, 24 h). Conclusions The study suggested that the glycosides of anthocyanins might handicap glucose transport and inhibit energy metabolism, which, in turn, led to mitochondrial damage and apoptosis of tumour cells.

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Section: Inhibitory Effects Of the Bilberry Anthocyanins Extract (Bae)mentioning

confidence: 99%

Glycosylation of anthocyanins enhances the apoptosis of colon cancer cells by handicapping energy metabolism

Jing

Song

Liu

et al. 2020

BMC Complement Med Ther

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“…Regardless of the used cell line, antiproliferative effects were spotted in a broad spectrum of concentrations from 5 to 1000 µg/mL. Hosseini et al [59] observed cytotoxic and proapoptotic effects of C. mas L. extract on AGS and L929 cell lines with the use of the MTT test and FITC-Annexin V binding, observed with the use of flow cytometry. Based on the figures featured in the mentioned study, the lowest concentrations of C. mas L. extract in which cytotoxicity could be observed were the following: 5 mg/mL (after 48 h of cell growth) or 2 mg/mL (after 72 h), regardless of the used cell line.…”

Section: Insights Into the In Vitro Antiproliferative And Cytotoxic P...mentioning

confidence: 99%

“…Much attention has been drawn to the cytotoxic, antiproliferative, and thus, anticancer [38,[56][57][58][59][60][61] attributes of C. mas L. Furthermore, the antitumor and anti-inflammatory actions of C. mas L. compounds have been successfully applied in the form of nanoparticle carriers containing the extract itself or its various components [9,[62][63][64][65][66]. Cytotoxic/antiproliferative properties of C. mas L. extracts have been observed (based on the aforementioned studies) with the use of various tumor cell lines, such as the following: MCF-7, SKOV-3, PC-3, HeLa, HepG2, CaCo-2, HT29, CT26, A549.…”

Section: Introductionmentioning

confidence: 99%

Cornelian Cherry (Cornus mas L.) Extracts Exert Cytotoxicity in Two Selected Melanoma Cell Lines—A Factorial Analysis of Time-Dependent Alterations in Values Obtained with SRB and MTT Assays

et al. 2022

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Despite the fact that phytochemicals of Cornaceae species have long been discussed as possible auxiliary agents in contemporary treatment, the insights on their properties remain relatively scarce. This study focuses on Cornus mas L. (Cornelian cherry), the extracts of which are reported to exert a pleiotropic effect shown in both in vivo and in vitro studies. This study aimed to explore the cytotoxic effect of extracts from fruits of red (Cornus mas L. ‘Podolski’) and yellow (Cornus mas L. ‘Yantarnyi’ and ‘Flava’) Cornelian cherries on two melanoma cell lines (A375 and MeWo). The extracts were characterized in the context of the concentration of bioactive compounds of antioxidative properties. Cytotoxicity was investigated with the use of the following two assays: SRB and MTT. An additional, alternative protocol for the SRB assay was used in this study so as to account for possible bias. Cytotoxicity was assessed as a difference in the whole time series of cell viability, instead of analyzing differences in raw values (often found in the literature). Both extracts from Cornus mas L. induced cytotoxicity in both A375 and MeWo cell lines, although the response of these cells was different. Moreover, based on this study, there is no evidence for claiming a different magnitude of cytotoxicity between these two extracts.

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“…Recent studies discovered that natural products can also trigger non-canonical cell death such as ferroptosis, necroptosis, and pyroptosis [33]. Among the studies discussed, one reported the occurrence of necrosis and another lipid peroxidation [34,35]. Other studies mainly reported induction of apoptosis and/or autophagy.…”

Section: Role Of Naturally Occurring Molecules In Gastric Cancer Managementsmentioning

confidence: 99%

Emerging Potential of Naturally Occurring Molecules against Gastric Cancer: Insights Molecular Mechanism and Therapeutic Targets

Kang¹,

Hwang²,

Shin³

et al. 2021

Preprint

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Gastric cancer, also known as stomach cancer, is a cancer which develops from the lining of the stomach. Accumulated evidences and epidemiological studies have been indicated that natural products play an important role in gastric cancer prevention and treatment, although its mechanism of action did not elucidate yet. Particularly, experimental studies have been showed that natural products displayed a protective effect against gastric cancer via numerous molecular mechanisms such as suppression of cell metastasis, anti-angiogenesis, inhibition of cell proliferation, induction of apoptosis, and modulation of autophagy. Although chemotherapy remains the standard treatment for advanced gastric cancer along with surgery, radiation therapy, hormone therapy and immunotherapy, but its adverse side effects including neutropenia, stomatitis, mucositis, diarrhea, nausea, and emesis are well documented. Additionally, intake of naturally occurring phytochemicals could increase the efficacy of gastric chemotherapy and chemotherapeutics resistance. However, natural product structural stability and powerful bioactivity are important to develop novel treatments for gastric cancer that may minimize such adverse effects. Therefore, the purpose of this review is to summarize the potential therapeutic effects of natural products on prevention and treatment of gastric cancer with intensive molecular mechanisms of action, bioavailability, and safety efficacy.

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Evaluating of Induction of Apoptosis by Cornus mass L. Extract in the Gastric Carcinoma Cell Line (AGS)

Cited by 12 publications

References 45 publications

Glycosylation of anthocyanins enhances the apoptosis of colon cancer cells by handicapping energy metabolism

Glycosylation of anthocyanins enhances the apoptosis of colon cancer cells by handicapping energy metabolism

Cornelian Cherry (Cornus mas L.) Extracts Exert Cytotoxicity in Two Selected Melanoma Cell Lines—A Factorial Analysis of Time-Dependent Alterations in Values Obtained with SRB and MTT Assays

Emerging Potential of Naturally Occurring Molecules against Gastric Cancer: Insights Molecular Mechanism and Therapeutic Targets

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