Evaluating micro-propagation of Kashan damask rose, Yasooj aromatic rose and their hybrid

Abstract

According to the value of damask rose and aromatic rose, crossing of Kashan damask rose × Yasooj aromatic rose has high importance in terms of its essential oil performance, rose water quality and its usage as a medicinal plant. With considering propagation problems of this plant, mass reproduction with tissue culture can help in a short time. The samples of hybrid plants and their parents were collected from Research Institute of Forests and Rangelands. After pre-sterilization and sterilization, explants were established on the MS medium. When length of explants became around 2 cm, were transferred to MS medium with 3 different treatments for shooting and it was indicated that best shooting treatment for all 3 genotypes was MS medium with IBA (0.1 mg/l), 2ip (0.5 mg/l) and BA (0.5 mg/l). G22 genotype produced better shoots, so it was established on 8 different rooting treatments and finally MS media with IBA (2 mg/l) showed best result (100%). Then explants of (G22×G4) and G4 genotype also were established on the best rooting media and produced 28% and 0% roots, respectively. All the rooted plantlets were transferred to plastic pots containing cocopeat+ perlite+ vermicompost with the ratio of 2:1:0.5 and placed in the laboratory for compatibility. Totally it was concluded that between 3 genotypes (crossed (G22×G4), damask rose as the paternal line (G4) and (G22) aromatic rose as the maternal line), G22 had the highest results of micropropagation, G4 had lowest results and crossed plant (G22×G4) was in the interstitial situation.

Clonal micropropagation of essential oil rose cultivars and breeding samples at long-term subcultivationin vitro

“…The formation of several shoots at the stage of explant introduction in vitro was noted in many works devoted to the micropropagation of essential oil roses [2,[8][9][10]12]. However, some authors noted that explants exude dark colored compounds into the culture medium (phenols, pigments) that are released from the cut ends of the explants [14].…”

“…When studying in vitro propagation of three cultivars of R. damascena and R. gallica, formation from 2.5 to 4.2 shoots per explant was reported [15]. S. Mirzaei et al at micropropagation of Kashan damask rose, Yasooj aromatic rose and their hybrid received up to 3.4-3.9 shoots per explant depending on genotype [8]. During multiplication in vitro of different R. damascena cultivars and samples it was reported obtaining 4.4-6.0 [13], 3.7-4.0 [11], 2.9-3.8 [12], 5.9 [5] shoots per explant.…”

“…There are far less works devoted to research on clonal micropropagation for essential oil roses than for ornamental ones. These publications mostly covered issues of R. damascena propagation in vitro [2,[5][6][7][8]. As explants when introduced into an aseptic culture, axillary or apical buds and meristems [5,[8][9][10], stem segments with a node [7,9,[11][12] were usually used.…”

“…These publications mostly covered issues of R. damascena propagation in vitro [2,[5][6][7][8]. As explants when introduced into an aseptic culture, axillary or apical buds and meristems [5,[8][9][10], stem segments with a node [7,9,[11][12] were usually used. There is evidence of the use of direct shoot regeneration from leaf, stem and petiole for R. damascena micropropagation [1].…”

“…B. Allahverdi Mamaghani et al [5] recorded the highest level of shoot multiplication rate at a combination of 5 mg/l BAP and 0.1 mg/l thidiazuron. For three genotypes (Kashan damask rose, Yasooj aromatic rose and their hybrid), the best shoot formation from shoot tip explants was achieved on MS medium with BAP (0.5 mg/l), N 6 -2isopentenyl adenine (0.5 mg/l) and IBA (0.1 mg/l) [8]. At the same time, M. Salekjalali [13] for R. damascena revealed the effectiveness of introducing into the medium not only BAP and NAA, but also phloroglucinol, which enhanced the multiplication rates in vitro.…”