Clonal micropropagation of essential oil rose cultivars and breeding samples at long-term subcultivation<i>in vitro</i>

Yegorova, N.A.; Stavtzeva, Irina

doi:10.1051/e3sconf/202022404010

Cited by 2 publications

(1 citation statement)

References 15 publications

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“…The composition of the culture medium holds paramount importance in lavender micropropagation, as it dictates the plant tissue's growth, morphological attributes, and phytochemical makeup [10]. Numerous studies have been dedicated to refining micropropagation protocols for lavender, revealing that shoot proliferation increases in initial subcultures but declines in subsequent ones [11,12]. Elicitor compounds such as jasmonic and salicylic acid, integrated into the culture medium, have positively impacted the growth and biochemical composition of in vitro propagated lavender [10].…”

Section: Introductionmentioning

confidence: 99%

Optimizing Micropropagation and Rooting Protocols for Diverse Lavender Genotypes: A Synergistic Approach Integrating Machine Learning Techniques

Şimşek,

Dalda Şekerci,

Isak

et al. 2024

Horticulturae

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This study comprehensively explored the micropropagation and rooting capabilities of four distinct lavender genotypes, utilizing culture media with and without 2 g/L of activated charcoal. A systematic examination of varying concentrations of BAP for micropropagation and IBA for rooting identified an optimal concentration of 1 mg/L for both BAP and IBA, resulting in excellent outcomes. Following robust root development, the acclimatization of plants to external conditions achieved a 100% survival rate across all genotypes. In addition to the conventional techniques employed, integrating machine learning (ML) methodologies holds promise for further enhancing the efficiency of lavender propagation protocols. Using cutting-edge computational tools, including MLP, RBF, XGBoost, and GP algorithms, our findings were rigorously examined and forecast using three performance measures (RMSE, R2, and MAE). Notably, the comparative evaluation of different machine learning models revealed distinct R2 rates for plant characteristics, with MLP, RBF, XGBoost, and GP demonstrating varying degrees of effectiveness. Future studies may leverage ML models, such as XGBoost, MLP, RBF, and GP, to fine-tune specific variables, including culture media composition and growth regulator treatments. The adaptability and ability of ML techniques to analyze complex biological processes can provide valuable insights into optimizing lavender micropropagation on a broader scale. This collaborative approach, combining traditional in vitro techniques with machine learning, validates the success of current micropropagation and rooting protocols and paves the way for continuous improvement. By embracing ML in lavender propagation studies, researchers can contribute to advancing sustainable and efficient plant propagation techniques, thereby fostering the preservation and exploitation of genetic resources for conservation and agriculture.

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Section: Introductionmentioning

confidence: 99%

Optimizing Micropropagation and Rooting Protocols for Diverse Lavender Genotypes: A Synergistic Approach Integrating Machine Learning Techniques

Şimşek,

Dalda Şekerci,

Isak

et al. 2024

Horticulturae

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Micropropagation in vitro of essential oil rose hybrids obtained in embryoculture

2021

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The aim of the work was to study the features of clonal micropropagation of essential oil rose interspecific hybrids obtained in embryo culture in vitro. Analysis of 12 crossing combinations demonstrated that the frequency of hybrid seedling formation in the embryo culture varied from 0 to 71.4%. For clonal micropropagation obtained in vitro seedlings were divided into stem segments with a node and cultivated on MS culture medium supplemented with 0.5 mg/l BAP and 0.1 mg/l GA3. During the multiplication of 13 hybrids (R. alba × R. damascena cv. ‘Kazanlykskaya’) in 2-6 subcultures, high variability of the multiplication index (1.8-18.5 depending on the genotype and passage) was revealed. This parameter was maximum in the 3-4th subcultures. The best ability to micropropagation showed hybrid No. 37-14. Microshoots were rooted in vitro on ½ MS medium, containing for different hybrids 0.5 or 1.0 mg/l NAA; frequency – up to 80.5-100.0%. However, in No. 37-2, 37-19 and 37-31 on four tested media, the number of shoots with roots was only 0-35.4%.

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Clonal micropropagation of essential oil rose cultivars and breeding samples at long-term subcultivationin vitro

Cited by 2 publications

References 15 publications

Optimizing Micropropagation and Rooting Protocols for Diverse Lavender Genotypes: A Synergistic Approach Integrating Machine Learning Techniques

Optimizing Micropropagation and Rooting Protocols for Diverse Lavender Genotypes: A Synergistic Approach Integrating Machine Learning Techniques

Micropropagation in vitro of essential oil rose hybrids obtained in embryoculture

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