Evaluating human sperm DNA integrity: relationship between 8-hydroxydeoxyguanosine quantification and the sperm chromatin structure assay

Oger, Isabelle; Cruz, Christelle Da; Panteix, G.; Ménézo, Yves

doi:10.1017/s0967199403002442

Cited by 59 publications

(39 citation statements)

References 21 publications

(32 reference statements)

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“…DNA fragmentation in mature male germ cell is usually caused by external factors such as reactive oxygen species (ROS), rather than cell's own programmed death [24]. Study has proven that DNA fragmentation measured by the SCSA originates (a part) from oxidative products (8-hydroxy-deoxyguanosine) [32]. It is therefore necessary to evaluate sperm DFI and the origin of increased DFI, so that a couple can be provided appropriate therapeutics.…”

Section: Discussionmentioning

confidence: 99%

Predictive value of DNA integrity analysis in idiopathic recurrent pregnancy loss following spontaneous conception

Kumar

Deka

Singh

et al. 2012

J Assist Reprod Genet

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Purpose Standard semen parameters are poor predictors of fertility potential. To date, apart from, paternal karyotyping sperm factors are not evaluated in recurrent pregnancy loss (RPL), only recent studies have emphasized the role of sperm factors in early embryonic development as sperm transcribes genes critical for early embryonic development. Sperm DNA integrity is useful diagnostic and prognostic marker and has clinical implications in idiopathic recurrent pregnancy loss (iRPL) following spontaneous conception. The aim of this study was to assess DNA integrity in cases experiencing iRPL following spontaneous conception. Methods Semen samples from 45 patients and 20 controls were analyzed as per WHO 1999 guidelines and sperm chromatin structure assay (SCSA) was used to measure DNA fragmentation index (DFI). Results By applying receiver operating curve (ROC) analysis, sperm DFI of approximately 26 % was found in male partner of couples experiencing iRPL. Conclusions Our data indicate that sperm from men with a history of iRPL have a higher percentage of DNA damage as compared to control group, and this can explain pregnancy loss in these patients. Men with higher DFI are infertile whereas men with lower DFI (26 %) are able to conceive but experience recurrent pregnancy loss. Thus it is important to evaluate sperm DFI in couples experiencing iRPL to understand exact aetiology of RPL and determine prognosis and management.

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Section: Discussionmentioning

confidence: 99%

Predictive value of DNA integrity analysis in idiopathic recurrent pregnancy loss following spontaneous conception

Kumar

Deka

Singh

et al. 2012

J Assist Reprod Genet

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“…Additionally, the presence of reactive oxygen species (ROS) and ROS-related DNA oxidative products, such as 8-hydroxy-deoxyguanosine (8-OHdG) have also been associated with sperm DNA fragmentation (17,25). It has been shown that as sperm concentration decreases, DNA fragmentation index and 8-OH-dG increase, suggesting that ROS and the relevant products may have a major impact on sperm DNA integrity (25).…”

Section: Discussionmentioning

confidence: 99%

“…It has been shown that as sperm concentration decreases, DNA fragmentation index and 8-OH-dG increase, suggesting that ROS and the relevant products may have a major impact on sperm DNA integrity (25).…”

Section: Discussionmentioning

confidence: 99%

The effects of sperm quality on embryo development after intracytoplasmic sperm injection

Loutradi

Tarlatzis

Goulis

et al. 2006

J Assist Reprod Genet

117

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Purpose:To explore the possible relationship between sperm quality and embryo development, pregnancy and implantation rates, in patients undergoing intracytoplasmic sperm injection (ICSI). Methods: Fertilization and cleavage rates, quality of embryos, blastocyst development, pregnancy and implantation rates were analyzed in 1020 embryos from 219 couples undergoing first ICSI treatment cycle. The couples were allocated in five groups, according to semen parameters: Group 1: patients with normal semen parameters, Group 2: patients with mild oligoastheno-teratozoospermia, Group 3: patients with severe oligo-astheno-teratozoospermia, Group 4: patients with obstructive azoospermia, Group 5: patients with non-obstructive azoospermia. Results: Fertilization and cleavage rates, quality of embryos as well as blastocyst development rates were significantly reduced, as semen quality decreased. However, no significant differences were observed in clinical pregnancy and implantation rates. Conclusion: Overall, a negative relationship was observed between semen quality and embryo development, even before activation of the embryonic genome, suggesting that sperm can affect embryogenesis from a very early stage.

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“…Sperm DNA damage can arise by different mechanisms, among which it is believed that the excess production of ROS plays a major role [Aitken et al 1998;Henkel et al 2005;Oger et al 2003;Saleh et al 2002a;Wang et al 2003]. Reactive oxygen species can originate from endogenous sources such as leukocytes and immature spermatozoa [Aitken and West 1990;Kessopoulou et al 1992;Shekarriz et al 1995], as well as, exogenous sources such as industrial compounds, smoking, and alcohol [Acharya et al 2003;Agarwal and Saleh 2002;Kasahara et al 2002;Koch et al 2004;Kothari et al 2010;Saleh et al 2002b].…”

Section: Introductionmentioning

confidence: 99%

Cytosolic and mitochondrial ROS: which one is associated with poor chromatin remodeling?

Kiani-Esfahani¹,

Bahrami²,

Tavalaee³

et al. 2013

Systems Biology in Reproductive Medicine

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The aim of this study was to determine if there are any associations between impaired chromatin packaging and the origin of reactive oxygen species (ROS) production. Cytosolic ROS, mitochondrial ROS, DNA protamination, and apoptosis were evaluated with dichlorofluoresceindiacetate (DCFH-DA), dihydrorhodamine 123 (DHR123), chromomycin A3 (CMA3), and YO-Pro-1 (Y1)/propidium iodide (PI), respectively, by flow cytometry (FCM) in 40 infertile individuals. Percentages DCF+ and R123+ sperm were positively associated with percentage CMA3+ sperm and negatively associated with percentage apoptotic sperm. No correlation was observed between CMA3+ sperm and the percentage of apoptotic sperm. Under protamination of sperm is not associated with the origin of ROS production, but their relationship may suggest an association with general physiological dysfunction of sperm. Furthermore, under protamination does make sperm prone to apoptosis. Rather, it is likely that apoptosis is induced by ROS production. Considering that these conclusions are derived from correlative analyses, additional studies including an interventive approach are required.

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Evaluating human sperm DNA integrity: relationship between 8-hydroxydeoxyguanosine quantification and the sperm chromatin structure assay

Cited by 59 publications

References 21 publications

Predictive value of DNA integrity analysis in idiopathic recurrent pregnancy loss following spontaneous conception

Predictive value of DNA integrity analysis in idiopathic recurrent pregnancy loss following spontaneous conception

The effects of sperm quality on embryo development after intracytoplasmic sperm injection

Cytosolic and mitochondrial ROS: which one is associated with poor chromatin remodeling?

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