Cytosolic and mitochondrial ROS: which one is associated with poor chromatin remodeling?

Kiani-Esfahani, Abbas; Bahrami, Solmaz; Tavalaee, Marziyeh; Deemeh, Mohammad Reza; Mahjour, Amir Ashkan; Nasr-Esfahani, Mohammad Hossein

doi:10.3109/19396368.2013.829536

Cited by 18 publications

(14 citation statements)

References 53 publications

(72 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…the efficiency of chromatin remodeling in the male line is in keeping with previous publications demonstrating that strong relationships exist between the efficiency of chromatin protamination, as measured by chromomycin 3 (CMA3) fluorescence, and both ROS generation and oxidative DNA damage-the authors of this paper cannot claim to be the first to make this link [8,9]. 4.…”

Section: The Observed Relationship Between Oxidative Stress Andsupporting

confidence: 86%

Oxidative stress and the etiology of male infertility

Aitken

2016

J Assist Reprod Genet

View full text Add to dashboard Cite

Section: The Observed Relationship Between Oxidative Stress Andsupporting

confidence: 86%

Oxidative stress and the etiology of male infertility

Aitken

2016

J Assist Reprod Genet

View full text Add to dashboard Cite

“…Poor protamination with elevated levels of peripheral histone retention would leave the chromatin far less dense and exposed to ROS attack [Hammoud et al 2011]. In fact, a positive correlation was found between poor protamination and higher levels of ROS production and subsequent DNA fragmentation in individuals with abnormal semen parameters [Kiani-Esfahani et al 2013]. Besides, the retention of histone is not random and is known to have epigenetic significance making it one of the important parameters to be studied in sperm of RPL [Brunner et al 2014;Castillo et al 2014].…”

Section: Introductionmentioning

confidence: 99%

Histone retention, protein carbonylation, and lipid peroxidation in spermatozoa: Possible role in recurrent pregnancy loss

Mohanty

Swain

Goswami

et al. 2016

Systems Biology in Reproductive Medicine

View full text Add to dashboard Cite

Contribution from a defective paternal genome has been attributed to be an important cause for spontaneous recurrent pregnancy loss (RPL). Increased oxidative stress results in decreased detoxification and is a cause for damage to chromatin, proteins, and membrane lipids. The present study aimed to explore if there is a significant relationship between retained histones due to defective packaging of DNA in spermatozoa and oxidative stress. RPL patients (n=16) with a history of ≥2 embryo losses before the 20th week of gestation and no female factor abnormality, and fertile healthy volunteers (n=20) as controls were included in the study. A significant difference in the levels of protein carbonylation and lipid peroxidation together with an increased retention of histones in the experimental groups was noticed. Histone carrying sites for oxidative modification such as arginine and lysine might be responsible for disturbing the paternal epigenomic control during early stages of embryonic differentiation leading to abortion. ARTICLE HISTORY

show abstract

“…Studies have demonstrated that inappropriate culture mediums increase ROS resulting in OS events, DNA damage and low quality of oocytes. These are the main factors that lead to failure in IVM and decrease productivity [9,10]. OS appears to be responsible for many injuries in the embryo and oocyte [9,11,12].…”

Section: Introductionmentioning

confidence: 99%

Effect of Antioxidants ( β -mercaptoethanol and Cysteamine) on Assisted Reproductive Technology In vitro

Nikseresht

Toori

Rahimi

et al. 2017

JCDR

View full text Add to dashboard Cite

IntrOductIOnIVM of oocytes is an effective method for the production of mature oocyte used in ART, which includes IVF cloning and Intra Cytoplasmic Sperm Injection (ICSI). IVM is a cost-benefit technique with few side effects for gonadotropin stimulation of IVF, and is a simple treatment for certain infertile couple [1][2][3][4]. The GV-stage oocytes which are stripped of cumulus cells have a reduced developmental capacity when compared with cumulus enclosed GV-stage oocytes [5,6]. Cumulus cells play an important role in oocyte maturation, since they provide and transfer several known and unknown factors that are essential in the regulation of meiotic progression, normal nuclear and cytoplasmic maturation of oocytes and subsequent embryonic development after fertilization [7,8]. Studies have demonstrated that inappropriate culture mediums increase ROS resulting in OS events, DNA damage and low quality of oocytes. These are the main factors that lead to failure in IVM and decrease productivity [9,10]. OS appears to be responsible for many injuries in the embryo and oocyte [9,11,12]. Embryo metabolism generates ROS via several enzymatic mechanisms that penetrate the cell membrane and pass through it to damage cellular molecules such as lipids, proteins, and nucleic acids [13]. BME and CYS are thiol compounds that stimulate glutathione (GSH) synthesis [14]. Despite the fact that there is more stability of CYS than other thiol compounds, the effect of this substance on the growth of oocytes or embryos depending on breed, dose rate and the medium, is shown differently.GSH is one of the components of many biological processes, which includes the construction of DNA, protein metabolism of drugs and chemicals, and it protects the cells during OS events [15]. In addition, GSH and its revival mode of reproduction and early development of organism play a unique role. It can reduce the generation of free radicals such as hydrogen peroxide and oxygen free radicals, which are disruptive to oocytes. It has been shown that GSH plays an important role in oocyte maturation [15]. Intracellular GSH is an essential part in the development of the oocyte cytoplasm. Activities of GSH-related antioxidant properties protect the oocyte at the front of the active molecules of oxygen is toxic. With this consideration, this study was designed to evaluate the effects of antioxidants (BME and CYS) on improving ART invitro which is essential. Therefore, the main purpose of this study was to ascertain whether enriching the oocyte medium with antioxidants, BME and CYS may improve IVM and fertilization and embryo development into blastocyst, of mouse immature oocyte. MAterIAls And MethOdsThis experimental study was carried out in the cellular and molecular research center at Yasuj University of Medical Sciences in Iran. The University ethic committee approved the design of study. Sixteen mice were housed under standard laboratory conditions (temperature of 20±2°C, relative humidity of 40-45% and light-dark cycle of 12:12 hour) and had free access ...

show abstract

Cytosolic and mitochondrial ROS: which one is associated with poor chromatin remodeling?

Cited by 18 publications

References 53 publications

Oxidative stress and the etiology of male infertility

Oxidative stress and the etiology of male infertility

Histone retention, protein carbonylation, and lipid peroxidation in spermatozoa: Possible role in recurrent pregnancy loss

Effect of Antioxidants ( β -mercaptoethanol and Cysteamine) on Assisted Reproductive Technology In vitro

Contact Info

Product

Resources

About