Evaluating Antibacterial Efficacy and Biocompatibility of PAN Nanofibers Loaded with Diclofenac Sodium Salt

Sarwar, Muhammad Nauman; Ullah, Azeem; Haider, Kaiser; Hussain, Nageen; Ullah, Sana; Hashmi, Motahira; Khan, Muhammad Qamar

doi:10.3390/polym13040510

Cited by 37 publications

(18 citation statements)

References 42 publications

(44 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this study, the antimicrobial activity of the electrospun pure PCL and DSS-loaded PCL mats was tested against S. aureus and E. coli by the contact method according to ISO 22196:2011. The selected bacterial strains are the most common bacteria that cause soft tissue infections that delay wound healing [76,77]. S. aureus most often causes skin infections [78], while E. coli is considered the primary source of burn wound infections [79].…”

Section: Discussionmentioning

confidence: 99%

The Drug-Loaded Electrospun Poly(ε-Caprolactone) Mats for Therapeutic Application

et al. 2021

View full text Add to dashboard Cite

Diclofenac sodium salt (DSS)-loaded electrospun nanofiber mats on the base of poly(ε-caprolactone) (PCL) were investigated as biocompatible nanofibrous mats for medical applications with the ability to inhibit bacterial infections. The paper presents the characteristics of fibrous mats made by electrospinning and determines the effect of medicament on the fiber morphology, chemical, mechanical and thermal properties, as well as wettability. PCL and DSS-loaded PCL nanofibrous mats were characterized using scanning electron microscopy, transmission electron microscopy, attenuated total reflectance-Fourier transform infrared spectrometry, dynamic mechanical analysis, and contact angle measurements. Electron paramagnetic resonance measurements confirmed the lifetime of DSS before and after application of high voltage during the electrospinning process. In vitro biocompatibility was studied, and it was proved to be of good viability with ~92% of the diploid human cells culture line composed of lung fibroblast (MRC 5) after 48 h of incubation. Moreover, the significant activity of DSS-loaded nanofibers against cancer cells, Ca Ski and HeLa, was established as well. It was shown that 12.5% (m/V) is the minimal concentration for antibacterial activity when more than 99% of Escherichia coli (Gram-negative) and 99% of Staphylococcus aureus (Gram-positive) have been exterminated.

show abstract

Section: Discussionmentioning

confidence: 99%

The Drug-Loaded Electrospun Poly(ε-Caprolactone) Mats for Therapeutic Application

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Furthermore, biological molecules can be incorporated into the polymer matrix 12 . Because of its outstanding properties such as chemical stability, light resistance, non-toxicity, electrospinning, and mechanical elasticity, polyacrylonitrile (PAN) is a popular polymer for wound dressing 13 .…”

Section: Introductionmentioning

confidence: 99%

Electrospun non-wovens potential wound dressing material based on polyacrylonitrile/chicken feathers keratin nanofiber

Serag

El-Aziz

El‐Maghraby

et al. 2022

Sci Rep

View full text Add to dashboard Cite

Electrospinning nanofibers have a tremendous interest in biomedical applications such as tissue engineering, drug administration, and wound healing because of their ability to replicate and restore the function of the natural extracellular matrix found in tissues. The study’s highlight is the electrospinning preparation and characterization of polyacrylonitrile with chicken feather keratin as an additive. In this study, keratin was extracted from chicken feather waste using an environmentally friendly method and used to reinforce polymeric nanofiber mats. Scanning electron microscopy, energy dispersive spectroscopy, and transmission electron microscopy were used to examine the morphology and the structure of the prepared nanofiber mats. The effect of keratin on the porosity and the tensile strength of reinforcing nanofibers is investigated. The porosity ratio of the nanofiber mats goes up from 24.52 ± 2.12 for blank polyacrylonitrile (PAN (NF)) to 90.89 ± 1.91% for polyacrylonitrile nanofiber with 0.05 wt% keratin (PAN/0.05% K). Furthermore, keratin reinforcement improves the nanofiber's mechanical properties, which are important for wound dressing application, as well as its antibacterial activity without causing hemolysis (less than 2%). The best antibacterial activities were observed against Pseudomonas aeruginosa (30 ± 0.17 mm inhibition zone) and Staphylococcus aureus (29 ± 0.31 mm inhibition zone) for PAN/0.05% K sample, according to the antibacterial test. This research has a good potential to broaden the use of feather keratin-based nanofibers in wound healing.

show abstract

“…Although the use of antibiotics to prevent infections is an effective solution, due to the occurrence of resistant pathogenic microorganisms and slower synthesis/isolation of new antibiotics, there is a need for alternative solutions [ 9 ]. Previous studies [ 23 , 24 , 25 , 26 ] have shown that diclofenac-incorporated formulations have antimicrobial activity, to some extent, against various bacterial strains. Since diclofenac primarily acts as an anti-inflammatory drug, which reduces post-injury pain, its additional advantage in wound treatment is its antibacterial property.…”

Section: Introductionmentioning

confidence: 99%

Curcumin and Diclofenac Therapeutic Efficacy Enhancement Applying Transdermal Hydrogel Polymer Films, Based on Carrageenan, Alginate and Poloxamer

Postolović

Antonijević²,

Ljujić³

et al. 2022

Polymers

View full text Add to dashboard Cite

Films based on carrageenan, alginate and poloxamer 407 have been formulated with the main aim to apply prepared formulations in wound healing process. The formulated films were loaded with diclofenac, an anti-inflammatory drug, as well as diclofenac and curcumin, as multipurpose drug, in order to enhance encapsulation and achieve controlled release of these low-bioavailability compounds. The obtained data demonstrated improved drug bioavailability (encapsulation efficiency higher than 90%), with high, cumulative in vitro release percentages (90.10% for diclofenac, 89.85% for curcumin and 95.61% for diclofenac in mixture-incorporated films). The results obtained using theoretical models suggested that curcumin establishes stronger, primarily dispersion interactions with carrier, in comparison with diclofenac. Curcumin and diclofenac-loaded films showed great antibacterial activity against Gram-positive bacteria strains (Bacillus subtilis and Staphylococcus aureus, inhibition zone 16.67 and 13.67 mm, respectively), and in vitro and in vivo studies indicated that curcumin- and diclofenac-incorporated polymer films have great potential, as a new transdermal dressing, to heal wounds, because diclofenac can target the inflammatory phase and reduce pain, whereas curcumin can enhance and promote the wound healing process.

show abstract

Evaluating Antibacterial Efficacy and Biocompatibility of PAN Nanofibers Loaded with Diclofenac Sodium Salt

Cited by 37 publications

References 42 publications

The Drug-Loaded Electrospun Poly(ε-Caprolactone) Mats for Therapeutic Application

The Drug-Loaded Electrospun Poly(ε-Caprolactone) Mats for Therapeutic Application

Electrospun non-wovens potential wound dressing material based on polyacrylonitrile/chicken feathers keratin nanofiber

Curcumin and Diclofenac Therapeutic Efficacy Enhancement Applying Transdermal Hydrogel Polymer Films, Based on Carrageenan, Alginate and Poloxamer

Contact Info

Product

Resources

About