Evaluación de métodos para el estudio de la sensibilidad a oxacilina y penicilina en 60 aislamientos de Staphylococcus lugdunensis

Batista, Nínive; Fernández, M. Paula; Lara, Magdalena; Laich, Federico; Mendez, Sebastian

doi:10.1016/j.eimc.2008.04.005

Cited by 8 publications

(4 citation statements)

References 16 publications

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“…The induced nitrocefin and penicillin MIC tests had equally high sensitivities and specificities. While the reference broth microdilution (BMD) penicillin MIC results correlated 100% with those of blaZ PCR, 259 (26.1%) isolates had penicillin MICs of 0.12 g/ml, near the susceptible breakpoint, which could prove problematic for automated antimicrobial susceptibility platforms that have a history of imperfect penicillin susceptibility detection (32)(33)(34)(35). However, no ␤-lactamase was detectable in tested isolates with penicillin MICs of Յ0.12 g/ml by reference broth microdilution, unlike S. aureus strains, which may harbor ␤-lactamase despite a susceptible penicillin MIC and thus require confirmation using the penicillin zone edge test (8).…”

Section: Discussionmentioning

confidence: 99%

Clinical and Microbiological Aspects of β-Lactam Resistance in Staphylococcus lugdunensis

et al. 2017

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Antimicrobial susceptibility results from broth microdilution MIC testing of 993 Staphylococcus lugdunensis isolates recovered from patients at a tertiary care medical center from 2008 to 2015 were reviewed. Ninety-two oxacillin-susceptible isolates were selected to assess the accuracy of penicillin MIC testing, the penicillin disk diffusion test, and three ␤-lactamase tests, including the cefoxitin-induced nitrocefin test, penicillin cloverleaf assay, and penicillin disk zone edge test. The results of all phenotypic tests were compared to the results of blaZ PCR. The medical records of 62 patients from whom S. lugdunensis was isolated, including 31 penicillinsusceptible and 31 penicillin-resistant strains, were retrospectively reviewed to evaluate the clinical significance of S. lugdunensis isolation, the antimicrobial agents prescribed, if any, and the clinical outcome. MIC testing revealed that 517/993 (52.1%) isolates were susceptible to penicillin and 946/993 (95.3%) were susceptible to oxacillin. The induced nitrocefin test was 100% sensitive and specific for the detection of ␤-lactamase compared to the blaZ PCR results, whereas the penicillin disk zone edge and cloverleaf tests showed sensitivities of 100% but specificities of only 9.1% and 89.1%, respectively. The penicillin MIC test had 100% categorical agreement with blaZ PCR, while penicillin disk diffusion yielded one major error. Only 3/31 patients with penicillin-susceptible isolates were treated with a penicillin family antimicrobial. The majority of cases were treated with other ␤-lactams, trimethoprimsulfamethoxazole, or vancomycin. These data indicate that nearly all isolates of S. lugdunensis are susceptible to narrow-spectrum antimicrobial agents. Clinical laboratories in areas with resistance levels similar to those described here can help promote the use of these agents versus vancomycin by effectively designing their antimicrobial susceptibility reports to convey this message.KEYWORDS Staphylococcus, antimicrobial resistance, beta-lactams, lugdunensis S taphylococcus spp. are normal skin flora inhabitants and, in some cases, opportunistic human pathogens (1). The coagulase-negative staphylococci (CoNS) are typically less virulent than Staphylococcus aureus in healthy human hosts. One exception is Staphylococcus lugdunensis, which may exhibit increased virulence compared to that of other CoNS (2). Infections associated with S. lugdunensis can include skin and soft tissue infections, native valve endocarditis, urinary tract infections, and prosthetic device infections (2).Isolates of CoNS, in particular Staphylococcus epidermidis, that harbor the mecA gene can have oxacillin MICs in the 0.5-to 2.0-g/ml range, which is much lower than the oxacillin MICs observed in mecA-positive isolates of S. lugdunensis and S. aureus (3-7). As such, the Clinical and Laboratory Standards Institute (CLSI) recommends that

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Section: Discussionmentioning

confidence: 99%

Clinical and Microbiological Aspects of β-Lactam Resistance in Staphylococcus lugdunensis

et al. 2017

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“…Nuestra tasa es similar a la publicada por Batista y cols. 13 , con cifras de 40% de resistencia a penicilina. La resistencia a oxacilina es infrecuente en este microorganismo (por debajo de 5%) 1,12 , lo que coincide con los datos de nuestro estudio.…”

Section: Discussionunclassified

Infección por Staphylococcus lugdunensis: descripción de 44 casos

Betrán

Cebollada

Lavilla

et al. 2022

Rev. chil. infectol.

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Introducción: Staphylococcus lugdunensis, es un estafilococo coagulasa negativa (SCN) con características de virulencia y de sensibilidad antimicrobiana que lo hacen más parecido a Staphylococcus aureus que a otros SCN. Objetivos: Conocer las características clínicomicrobiológicas de los aislados de S. lugdunensis identificados en nuestra institución. Material y Métodos: Se realizó un estudio retrospectivo de los aislados de S. lugdunensis entre los años 2017 y 2019 en

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“…Caso o teste seja positivo, o isolado é resistente a penicilina, carbóxi, ureído e aminopenicilinas, devendo-se realizar o teste com o disco de cefoxitina para verificar a suscetibilidade aos outros agentes betalactâmicos. A porcentagem de produção de betalactamases em S. lugdunensis varia de 7% a 40% (2,5,12) . Desde 2004, o Clinical Laboratory Standards Institute (CLSI) preconizou o uso do disco de cefoxitina em substituição à oxacilina para predizer a presença do gene mecA.…”

Section: Testes De Suscetibilidadeunclassified

“…O isolado possuidor do gene mecA produz uma proteína alterada de parede chamada proteína ligadora de penicilina (PBP2a), inviabilizando a terapêutica com qualquer agente betalactâmico. Por isso é fundamental a detecção do gene mecA, seja por características fenotípicas (resistência à cefoxitina ou verificação da presença de PBP 2a mediante testes de aglutinação em látex) ou genotípicas (PCR para detecção do gene mecA) (2,5) .…”

Section: Testes De Suscetibilidadeunclassified

Staphylococcus lugdunensis: um olhar diferenciado no laboratório clínico

Silveira¹,

d’Azevedo²

2011

J. Bras. Patol. Med. Lab.

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Evaluación de métodos para el estudio de la sensibilidad a oxacilina y penicilina en 60 aislamientos de Staphylococcus lugdunensis

Cited by 8 publications

References 16 publications

Clinical and Microbiological Aspects of β-Lactam Resistance in Staphylococcus lugdunensis

Clinical and Microbiological Aspects of β-Lactam Resistance in Staphylococcus lugdunensis

Infección por Staphylococcus lugdunensis: descripción de 44 casos

Staphylococcus lugdunensis: um olhar diferenciado no laboratório clínico

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