Infección por Staphylococcus lugdunensis: descripción de 44 casos

Betrán, Ana Pilar; Cebollada, Rocío; Lavilla, Ma José; Torres, Luís

doi:10.4067/s0716-10182022000200254

Cited by 1 publication

(1 citation statement)

References 10 publications

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“…S. lugdunensis has been represented as a "wolf in sheep's clothing" as it causes many serious diseases, such as skin and soft tissue infections (SSTI), subcutaneous tissue infections, bone and joint infections, prosthetic joint infections (PJI), vascular catheter-related infections, infective endocarditis (IE), bacteremia and abscesses [3][4][5][6][7][8][9][10]. Although S. lugdunensis is susceptible to most antibiotics [11][12][13][14] and lacks many of the virulence factors that S. aureus possesses, such as protein A, enterotoxins A, B or C, toxic shock syndrome toxin (TSST), hemagglutinin and toxin [1,15], this wolf in disguise appears to be equipped with other virulence factors and is capable of expressing pathological mechanisms. S. lugdunensis can adhere to host matrix proteins with surface adhesins such as the fibrinogen-binding surface protein (Fbl) [16], the putative fibronectin/fibrinogen binding proteins (fbpA), the von Willebrand factor binding protein (vWbf) [17], and the polysaccharide intercellular adhesin (ica) [18] and can produce some cytolytic toxins toward human erythrocytes such as the putative βhemolysin [19], the putative hemolysin III [20,21], and the small cytolytic S. lugdunensis synergistic hemolysins (SLUSH), peptides with δ-toxin-like activity [9,17].…”

Section: Introductionmentioning

confidence: 99%

Searching for Virulence Factors among Staphylococcus lugdunensis Isolates from Orthopedic Infections: Correlation of β-hemolysin, hemolysin III, and slush Genes with Hemolytic Activity and Synergistic Hemolytic Activity

Ravaioli,

Campoccia,

Mirzaei

et al. 2023

IJMS

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Staphylococcus lugdunensis is an emerging high-virulent pathogen. Here, the presence and expression of virulence genes (icaA, fbl, vwbl, fbpA, slush A, B and C, and genes of the putative β-hemolysin and hemolysin III) and the ability to induce synergistic hemolytic activity and hemolysis after 24, 48 and 72 h were investigated in a collection of twenty-two S. lugdunensis clinical isolates. The collection of isolates, mainly from implant orthopedic infections, had previously been grouped by ribotyping/dendrogram analysis and studied for biofilm matrices, biomasses and antibiotic resistances. Two isolates, constituting a unique small ribogroup sharing the same cluster, exhibited an amplicon size of the slush operon (S. lugdunensis synergistic hemolysin) which was shorter than the expected 977 bp. This outcome can predict the genetic lineage of the S. lugdunensis strains. One isolate (cra1342) presented two deletions: one of 90 bp in slush A and the other of 91 bp in slush B. Another isolate (N860314) showed a single 193 bp deletion, which encompassed part of the slush B terminal sequence and most of slush C. The isolate N860314 was devoid of hemolytic activity after 24 h, and the first consideration was that the deleted region deals with the coding of the active enzymatic site of the slush hemolysin. On the other hand, cra1342 and N860314 isolates with different slush deletions and with hemolytic activity after 24 and 48 h, respectively, could have replaced the hemolytic phenotype through other processes.

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