EUS-Guided Portal Venous Sampling of Circulating Tumor Cells

Chapman, Christopher G.; Waxman, Irving

doi:10.1007/s11894-019-0733-2

Cited by 20 publications

(15 citation statements)

References 31 publications

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“…portal venous as the main drain tube of pancreas with abundant CTCs and the promising clinical application value of portal venous CTCs test had been demonstrated by previous studies (4,7,9,37). Recently, ultrasound-guided ( 9) and endoscopic ultrasound-guided (39,40) fine-needle aspiration have gradually been used to obtain portal venous blood in advanced PDAC patients. However, portal vein puncture is an invasive approach with the possibility of bleeding、 infection、thrombotic、tumor cell spread.…”

Section: Discussionmentioning

confidence: 99%

Portal Venous Circulating Tumor Cells Undergoing Epithelial-Mesenchymal Transition Exhibit Distinct Clinical Significance in Pancreatic Ductal Adenocarcinoma

Pan

Yang

et al. 2021

Front. Oncol.

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BackgroundMuch importance is attached to the clinical application value of circulating tumor cells (CTCs), meanwhile tumor-proximal CTCs detection has interested researchers for its unique advantage. This research mainly discusses the correlation of portal venous (PoV) CTCs counts in different epithelial-mesenchymal transition status with clinicopathologic parameters and postoperative prognosis in resectable pancreatic ductal adenocarcinoma patients (PDAC).MethodsPDAC patients (n=60) who received radical resection were enrolled in this research. PoV samples from all patients and peripheral venous (PV) samples from 32 patients among them were collected to verify spatial heterogeneity of CTCs distribution, and explore their correlation with clinicopathologic parameters and clinical prognosis.ResultsCTCs detectable rate and each phenotype count of PoV were higher than those of PV. Patients with recurrence had higher PV and PoV epithelial CTCs (E-CTCs) counts than recurrence-free patients (P<0.05). Some unfavourable clinicopathologic parameters were closely related to higher PoV CTCs counts. Multivariate regression analysis demonstrated that PoV mesenchymal CTC (M-CTC)s≥1/5 ml was an independent risk factor for metastasis free survival (MFS) (P=0.003) and overall survival (OS) (P=0.043).ConclusionsOur research demonstrated that portal venous was a preferable vessel for CTC test, and patients with PoV M-CTC≥1/5 ml had shorter MFS and OS time in resectable PDAC patients. PoV CTC phenotype detection has the potential to be a reliable and accurate tool to identify resectable PDAC patients with high tendency of postoperative metastasis for better stratified management.

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Section: Discussionmentioning

confidence: 99%

Portal Venous Circulating Tumor Cells Undergoing Epithelial-Mesenchymal Transition Exhibit Distinct Clinical Significance in Pancreatic Ductal Adenocarcinoma

Pan

Yang

et al. 2021

Front. Oncol.

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“…Endoscopic ultrasound (EUS) provides high-resolution, precise access to portal vein allowing for minimally invasive access for portal vein blood sampling. 18 Catenacci et al first reported that portal vein blood can be acquired safely, and an increased number of CTC in the portal vein blood was found compared with peripheral circulation in PC patients. 19 However, due to the relatively small sample size and heterogeneity of the patients, they did not further investigate the correlation between clinical features and CTCs in the portal vein blood.…”

Section: Introductionmentioning

confidence: 99%

Endoscopic Ultrasound-Guided Acquisition of Portal Venous Circulating Tumor Cells as a Potential Diagnostic and Prognostic Tool for Pancreatic Cancer

Zhang¹,

Su²,

Wang³

et al. 2021

CMAR

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Background: Circulating tumor cells (CTCs) were a promising liquid biopsy for pancreatic cancer (PC) but circulate in low counts in peripheral blood. We evaluated the diagnostic and prognostic values of portal vein (PoV) CTCs in PC patients. Methods: PoV was aspirated under EUS guidance from 40 patients with suspected pancreaticobiliary cancers. Epithelial-mesenchymal-transition-related subtypes of CTCs were identified via immunofluorescence using EpCAM and Twist antibodies. The diagnostic and prognostic performance of PoV CTCs was investigated by receiver-operating characteristic (AUC) curve and Kaplan-Meier survival analysis. Results: In total, 40 patients including 31 with PC, 4 with non-pancreatic periampullary cancer and 5 with benign pancreatic diseases (BPD) were enrolled. CTCs were detected more in PoV compared with peripheral blood. PoV CTC numbers in BPD patients were lower than in PC patients. The number of PoV CTCs, especially mesenchymal-CTCs (M-CTCs), was positively correlated with the tumor burden, instead of epithelial-CTCs (E-CTCs). The combination of PoV CTC numbers and CA19-9 demonstrated better diagnostic efficiency (AUC value 0.987) than either alone in differentiating PC with BPD. Moreover, the diagnostic efficacy of PoV CTCs and M-CTCs were obviously better than that of E-CTCs and CA19-9 in distinguishing early and late stage PC. Lastly, high PoV CTC and M-CTC numbers were both associated with shorter overall survival. Conclusion: Acquisition of the PoV samples in PC patients via EUS-guided procedures has been proved safe and feasible. PoV CTCs, especially M-CTCs, have great potentials in diagnosing and predicting the prognosis of PC, especially in combination with CA19-9.

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“…38 EUS-acquired CTC samples of the PV could be utilized for molecular testing, clinical predication of possible future hepatic metastasis, and drug sensitivity analyses. 39…”

Section: Endoscopic Ultrasound-guided Portal Vein Sampling Of Circulamentioning

confidence: 99%

“…Ting et al showed that CTCs were found in PV blood samples in 58% of patients undergoing a Whipple procedure for pancreatic cancer [ 38 ]. EUS-acquired CTC samples of the PV could be utilized for molecular testing, clinical predication of possible future hepatic metastasis, and drug sensitivity analyses [ 39 ].…”

Section: Endoscopic Ultrasoundguided Portal Vein Sampling Of Circulatmentioning

confidence: 99%

Endoscopic Ultrasound-Guided Vascular Procedures: A Review

2020

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Since the 1980s, endoscopic ultrasound has advanced from being purely diagnostic to an interventional modality. The gastrointestinal tract offers an exceptional window for assessing the vascular structures in the mediastinum and in the abdomen. This has led to a rapidly growing interest in endoscopic ultrasound-controlled vascular interventions as a minimally invasive alternative to surgical and radiological procedures.

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EUS-Guided Portal Venous Sampling of Circulating Tumor Cells

Cited by 20 publications

References 31 publications

Portal Venous Circulating Tumor Cells Undergoing Epithelial-Mesenchymal Transition Exhibit Distinct Clinical Significance in Pancreatic Ductal Adenocarcinoma

Portal Venous Circulating Tumor Cells Undergoing Epithelial-Mesenchymal Transition Exhibit Distinct Clinical Significance in Pancreatic Ductal Adenocarcinoma

Endoscopic Ultrasound-Guided Acquisition of Portal Venous Circulating Tumor Cells as a Potential Diagnostic and Prognostic Tool for Pancreatic Cancer

Endoscopic Ultrasound-Guided Vascular Procedures: A Review

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