European Committee on Antimicrobial Susceptibility Testing (EUCAST) Technical Notes on antimicrobial susceptibility testing

Abstract

The main objectives of the European Committee on Antimicrobial Susceptibility Testing (EUCAST) are to harmonise breakpoints for antimicrobial agents in Europe, and to act as the breakpoint committee for the European Medicines Agency (EMEA) during the registration of new antimicrobial agents. Detailed EUCAST procedures for harmonising and setting breakpoints for antimicrobial agents are available on the EUCAST website. Beginning with the current issue, a series of EUCAST Technical Notes will be published in CMI, based on the rationale documents produced by EUCAST for each of the antimicrobial agents studied, with the aim of highlighting important background information underlying decisions on breakpoints made by EUCAST.

Resistance Rate and Minimum Inhibitory Concentration of Metronidazole Among Helicobacter pylori Strains in Tehran, Iran

“…Susceptibility of the bacterial strains was determined based on guidelines of the European committee on antimicrobial susceptibility testing (EUCAST) (14). Antimicrobial susceptibility testing was performed using the agar dilution method.…”

“…The EUCAST clinical breakpoint of > 8 µg/mL was defined for evaluation of the resistant strains and MIC values were recorded in each case (14). Helicobacter pylori strain RIGLD 245 was used as a reference strain for all the experiments.…”

Testing the optimality properties of a dual antibiotic treatment in a two-locus, two-allele model

“…Antimicrobial susceptibility tests can be difficult to standardize [38], but they are widely used to determine important quantitative pharmacodynamic parameters characterizing the inhibitory relationship between antibiotic and bacteria [39]. For bacteriostatic antibiotics, a dose-response curve that showing densities at a given timepoint at a series of increasing dosages characterizes the effect of the drug.…”

Intrinsic and Acquired Methicillin-Resistance Detection in Staphylococcus aureus and Its Relevance in Therapeutics

“…The antibiotic susceptibility test was performed by disk agar diffusion and agar dilution methods (7,9). The antibiotic susceptibility patterns of 105 S. aureus isolates toward 13 antibiotics were studied by the disk agar diffusion method using the following antibiotics (HiMedia): ciprofloxacin (5 µg), rifampin (5 µg), clindamycin (2 µg), cephalothin (30 µg), sulfamethoxazole (25 µg), amikacin (30 µg), erythromycin (15 µg), penicillin (10 U), cephalexin (30 µg), gentamicin (10 µg), lincomycin (2 µg), and chloramphenicol (30 µg).…”