Eucalyptus urograndis stem proteome is responsive to short-term cold stress

Abstract

Eucalyptus urograndis is a hybrid eucalyptus of major economic importance to the Brazilian pulp and paper industry. Although widely used in forest nurseries around the country, little is known about the biochemical changes imposed by environmental stress in this species. In this study, we evaluated the changes in the stem proteome after short-term stimulation by exposure to low temperature. Using two-dimensional gel electrophoresis coupled to high-resolution mass spectrometry-based protein identification, 12 proteins were found to be differentially regulated and successfully identified after stringent database searches against a protein database from a closely related species (Eucalyptus grandis). The identification of these proteins indicated that the E. urograndis stem proteome responded quickly to low temperature, mostly by down-regulating specific proteins involved in energy metabolism, protein synthesis and signaling. The results of this study represent the first step in understanding the molecular and biochemical responses of E. urograndis to thermal stress.

Proteomics: A tool to decipher cold tolerance

“…Cold stress in E. urograndis stems showed downregulation of protein cytochrome b5-like heme/steroid binding domain protein responsible for cell division and elongation (Leonardi et al 2015). The increased accumulation of this protein in Arabidopsis thaliana membrane caused small sized hypocotyls, therefore supporting a strong correlation in between steroid binding proteins and cell elongation (Yang et al 2005).…”

“…During cold stress, Eucalyptus shows differential regulation of various proteins performing different cellular functions. Proteins like fructose bisphosphate aldolase, glyceraldehyde 3-phosphate dehydrogenase and NADH ubiquinone oxidoreductase were downregulated after induction of cold stress suggesting slow-down of metabolic activity in case of E. urogrand stems (Leonardi et al 2015). Also, cold stress resulted in down regulation of a myb-like transcription factor in E. urograndis stems, a protein known as a receptor for activated C kinase (RACK), suggesting a possible role of this protein in temperature-sensing signaling pathways.…”

Seasonal Variation of Carbon Metabolism in the Cambial Zone of Eucalyptus grandis

“…UGPase activity is related to cell wall synthesis and it promotes carbohydrate accumulation in storage tissues under low temperatures (Kleczkowski et al, 2004 ). Proteins of carbohydrate and energy metabolism have been reported in the stems of white spruce (Galindo-González et al, 2012 ), Populus (Plomion et al, 2006 ; Kalluri et al, 2009 ; Durand et al, 2011 ), Eucalyptus (Celedon et al, 2007 ; Leonardi et al, 2015 ), and Pinus (Gion et al, 2005 ). In summer samples, we also identified an upregulation of proteins involved in cell wall synthesis and cytoskeleton (Table 1 ).…”

“…Celedon et al ( 2007 ) and Carvalho et al ( 2008 ) reported the identification of proteins and transcripts related to carbon metabolism in the cambial zone of E. grandis trees at different ages, including alcohol dehydrogenase and pyruvate decarboxylase. Leonardi et al ( 2015 ) identified stem proteins (e.g., energy metabolism) potentially related to cold stress responses in Eucalyptus urograndis . On the other hand, Durand et al ( 2011 ) found two sucrose synthase more abundant in the cambial zone of Populus tremula L. × P. alba L. submitted to drought stress.…”

Understanding C4 photosynthesis in Setaria by a proteomic and kinetic approach

“…Finally, proteins (35 µg per well) were loaded on a 10% (w/v) polyacrylamide gel for SDS-PAGE (Laemmli, 1970) and then run for 1 cm. Bands were visualized by staining with colloidal Coomassie Brilliant Blue G-250 and subsequent incubation with a 30% (v/v) methanol solution, and then cut with a sterile scalpel (Leonardi et al, 2015). Four pools of each sample (M and BS) were processed as biological quadruplicates.…”