Proteomics: A tool to decipher cold tolerance

Jan, Nelofer; Qazi, Hilal Ahmad; Raja, Vaseem; John, Riffat

doi:10.1007/s40626-019-00140-2

Cited by 13 publications

(5 citation statements)

References 291 publications

(277 reference statements)

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“…Environmental conditions affect the stability and activity of proteins. In order to understand how the plant responds to different environments, it is crucial to analyze changes in the proteome ( Nelofer et al., 2019 ) and PTMomes, including phosphoproteome ( Kosová et al., 2021 ). The cold stress response in cucumbers is a complex process involving a large number of genes and proteins.…”

Section: Discussionmentioning

confidence: 99%

Proteome and phosphoproteome analysis of 2,4-epibrassinolide-mediated cold stress response in cucumber seedlings

Zhou

Li²,

Yan³

et al. 2023

Front. Plant Sci.

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The 2, 4-epibrassinolide (EBR) significantly increased plants cold tolerance. However, mechanisms of EBR in regulating cold tolerance in phosphoproteome and proteome levels have not been reported. The mechanism of EBR regulating cold response in cucumber was studied by multiple omics analysis. In this study, phosphoproteome analysis showed that cucumber responded to cold stress through multi-site serine phosphorylation, while EBR further upregulated single-site phosphorylation for most of cold-responsive phosphoproteins. Association analysis of the proteome and phosphoproteome revealed that EBR reprogrammed proteins in response to cold stress by negatively regulating protein phosphorylation and protein content, and phosphorylation negatively regulated protein content in cucumber. Further functional enrichment analysis of proteome and phosphoproteome showed that cucumber mainly upregulated phosphoproteins related to spliceosome, nucleotide binding and photosynthetic pathways in response to cold stress. However, different from the EBR regulation in omics level, hypergeometric analysis showed that EBR further upregulated 16 cold-up-responsive phosphoproteins participated photosynthetic and nucleotide binding pathways in response to cold stress, suggested their important function in cold tolerance. Analysis of cold-responsive transcription factors (TFs) by correlation between proteome and phosphoproteome showed that cucumber regulated eight class TFs may through protein phosphorylation under cold stress. Further combined with cold-related transcriptome found that cucumber phosphorylated eight class TFs, and mainly through targeting major hormone signal genes by bZIP TFs in response to cold stress, while EBR further increased these bZIP TFs (CsABI5.2 and CsABI5.5) phosphorylation level. In conclusion, the EBR mediated schematic of molecule response mechanisms in cucumber under cold stress was proposed.

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Section: Discussionmentioning

confidence: 99%

Proteome and phosphoproteome analysis of 2,4-epibrassinolide-mediated cold stress response in cucumber seedlings

Zhou

Li²,

Yan³

et al. 2023

Front. Plant Sci.

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“…Earlier developing stress-tolerant varieties via conventional breeding was laborious and time-consuming process which was eased down by the new modern breeding approaches as well as by multi-omic techniques that simplify the improvement and development of new cold stress-tolerant varieties in various crops. Thus, to survive the cold stress, plants adapt different approaches which include gene expression, reprogramming, and alteration in different metabolic processes which help in modulation of various proteins of stress induction (Jan et al, 2019). In-depth knowledge of how plants react to cold stress could provide critical information and biological resources for improving crop cold-stress tolerance.…”

Section: Introductionmentioning

confidence: 99%

Low Temperature Stress Tolerance: An Insight Into the Omics Approaches for Legume Crops

Bhat

Mahajan²,

Pakhtoon³

et al. 2022

Front. Plant Sci.

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The change in climatic conditions is the major cause for decline in crop production worldwide. Decreasing crop productivity will further lead to increase in global hunger rate. Climate change results in environmental stress which has negative impact on plant-like deficiencies in growth, crop yield, permanent damage, or death if the plant remains in the stress conditions for prolonged period. Cold stress is one of the main abiotic stresses which have already affected the global crop production. Cold stress adversely affects the plants leading to necrosis, chlorosis, and growth retardation. Various physiological, biochemical, and molecular responses under cold stress have revealed that the cold resistance is more complex than perceived which involves multiple pathways. Like other crops, legumes are also affected by cold stress and therefore, an effective technique to mitigate cold-mediated damage is critical for long-term legume production. Earlier, crop improvement for any stress was challenging for scientific community as conventional breeding approaches like inter-specific or inter-generic hybridization had limited success in crop improvement. The availability of genome sequence, transcriptome, and proteome data provides in-depth sight into different complex mechanisms under cold stress. Identification of QTLs, genes, and proteins responsible for cold stress tolerance will help in improving or developing stress-tolerant legume crop. Cold stress can alter gene expression which further leads to increases in stress protecting metabolites to cope up the plant against the temperature fluctuations. Moreover, genetic engineering can help in development of new cold stress-tolerant varieties of legume crop. This paper provides a general insight into the “omics” approaches for cold stress in legume crops.

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“…quadrangulosa, E. glaucescens, E. moorei, E. subcrenulata and E. nitida, cold stratification reduced germination rates compared to unstratified seeds. Cold stress not only reduces the yield but also restricts the geographical distribution of many plants, environmental conditions greatly influence the accumulation of many proteins and thus dissecting the dynamics of proteome in response to any external stimuli (Jan et al 2019). In contrast, germination of seeds of one species, E. mitchelliana, increased following a four-week cold-stratification period.…”

Section: Correlations Between Tz Seed Viability Vigour and Seed Massmentioning

confidence: 99%

A comparative study on seed physiology and germination requirements for 15 species of Eucalyptus

Afroze

Douglas

Grogan

2021

Theor. Exp. Plant Physiol.

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Seed physiology of 15 Eucalyptus species of interest for cut foliage plantations was unknown and therefore evaluated. The viability and vigour of seeds and germination potential of 15 Eucalyptus species was determined by using a tetrazolium (TZ) staining test, and the results were compared to a germination test. In a separate experiment, seeds of each lot were subjected to either 0 or 4-week cold stratification at 4 ± 1 °C to investigate their potential stratification requirement. After stratification, seeds were then allowed to germinate at 22 ± 1 °C with 16 h lighting per day for 36 days. Seed viability and vigour were checked by evaluating % root, cotyledon and first true leaves emergence, and the speed of emergence, in the germination test. The germination percentages varied with the species. Seed stratification with the interaction of seed species lots significantly affected both viability and vigour. The seed viability of the different species ranged from 9 to 100% and 2 to 100%, for the TZ test and germination test, respectively, with a high correlation (R2 = 0.89) between the two. Physiology tests revealed that cold stratification of seed was not required for the 15 species to maximise their germination potential and growth in Irish and British climate.

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Proteomics: A tool to decipher cold tolerance

Cited by 13 publications

References 291 publications

Proteome and phosphoproteome analysis of 2,4-epibrassinolide-mediated cold stress response in cucumber seedlings

Proteome and phosphoproteome analysis of 2,4-epibrassinolide-mediated cold stress response in cucumber seedlings

Low Temperature Stress Tolerance: An Insight Into the Omics Approaches for Legume Crops

A comparative study on seed physiology and germination requirements for 15 species of Eucalyptus

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