Eubacterial PCR for Bacterial Detection and Identification in 100 Acute Postcataract Surgery Endophthalmitis

Chiquet, Christophe; Cornut, Pierre-Loïc; Bénito, Yvonne; Thuret, Gilles; Maurin, Max; Lafontaine, P.O.; Péchinot, A.; Palombi, Karine; Lina, Gérard; Bron, Alain M.; Denis, Philippe; Carricajo, Anne; Creuzot, C; Romanet, Jean-Paul; Vandenesch, François

doi:10.1167/iovs.07-1377

Cited by 116 publications

(94 citation statements)

References 28 publications

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“…On the other hand, PCR was capable of detecting bacterial DNA in 37 (63.8%) of the specimens tested, demonstrated 100% concordance with cases characterized microbiologically by culture, and was capable of detecting bacterial DNA in 44.7% of the specimens found negative for bacterial culture. Later studies were able to observe a sensitivity of detection of pathogens by PCR varying from 57% to 100% while sensitivity of the culture varied between 24% and 56% (11,(13)(14)(15)22) .…”

Section: Molecular Detection and Identification In Cases Of Bacterialmentioning

confidence: 99%

“…Soon after, various authors showed how the utilization of PCR for the direct detection of pathogens from aqueous and vitreous humor samples could impact effectively the diagnosis of bacterial endophthalmitis, mainly by increasing significantly the number of cases that were characterized microbiologically with the use of PCR but that were shown to be negative by microscopy or culture (11,(13)(14)(15)(19)(20)(21)(22) (Table 2). The nested PCR method was later used in specimens of vitreous (n=30) and aqueous (n=28) humor collected from 55 patients with clinical diagnosis of endophthalmitis (21) .…”

Section: Molecular Detection and Identification In Cases Of Bacterialmentioning

confidence: 99%

“…A multicenter study published recently where PCR was applied for the diagnosis of bacterial endophthalmitis, included 100 patients with acute endophthalmitis following cataract surgery who were treated in 4 academic hospitals in France (22) . The specimens were divided into two groups, those collected at the time of admission and before the initiation of antibiotic therapy (n=76 for AH and n=38 for VH) and those collected at a second time (n=53 for AH and n=57 for VH) only in patients who required another intravitreal injection of antibiotic or were submitted to vitrectomy via pars plana.…”

Section: Molecular Detection and Identification In Cases Of Bacterialmentioning

confidence: 99%

See 2 more Smart Citations

Molecular biology applied to the laboratory diagnosis of bacterial endophthalmitis

Bispo

Höfling-Lima

Pignatari

2009

Arq. Bras. Oftalmol.

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Section: Molecular Detection and Identification In Cases Of Bacterialmentioning

confidence: 99%

Section: Molecular Detection and Identification In Cases Of Bacterialmentioning

confidence: 99%

Section: Molecular Detection and Identification In Cases Of Bacterialmentioning

confidence: 99%

See 1 more Smart Citation

Molecular biology applied to the laboratory diagnosis of bacterial endophthalmitis

Bispo

Höfling-Lima

Pignatari

2009

Arq. Bras. Oftalmol.

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“…Bacterial ribosomal DNA genes (16S rDNA) or fungal ribosomal DNA genes (18S or 28S rDNA) were previously used for qualitative broad-range PCR performed with ocular fluids of patients with infectious endophthalmitis and uveitis [15][16][17][18]. Broad-range PCR techniques use primers and probes for conserved regions in the genomes of bacteria and fungi of many species.…”

Section: Broad-range Real-time Pcrmentioning

confidence: 99%

“…In clinical settings, it is common practice to treat patients with endophthalmitis or infectious uveitis with antibiotics before samples are collected for culture, smear, and/or PCR tests. In these clinical situations, PCR is more effective than cultures and smears in detecting bacterial and fungal genomes in the ocular fluids [18] because PCR may still be able to detect the infectious DNA of living or dead bacteria and fungi. However, previous broad-range PCR techniques for bacteria and fungi were not able to determine quantitative measurements of these genomes in ocular samples.…”

Section: Broad-range Real-time Pcrmentioning

confidence: 99%

A new era of uveitis: impact of polymerase chain reaction in intraocular inflammatory diseases

et al. 2016

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Uveitis is a sight-threatening intraocular inflammatory disorder which may occur from both infectious and non-infectious or autoimmune causes. The frequency of infectious uveitis and autoimmune uveitis varies depending on countries and regions. According to a nationwide survey conducted by the Japanese Ocular Inflammation Society, infectious and non-infectious uveitis accounted for 16.4 and 50.1% of new patients, respectively while the remaining 33.5% of new uveitis cases were not classified or were idiopathic uveitis. Infectious uveitis is particularly important because it causes tissue damage to the eye and may result in blindness unless treated. However, it can be treated if the pathogenic microorganisms are identified promptly and accurately. Remarkable advancements in molecular and immunological technologies have been made in the last decade, and the diagnosis of infectious uveitis has been greatly improved by the application of molecular and immunological investigations, particularly polymerase chain reaction (PCR). PCR performed on a small amount of ocular samples provides a prompt, sensitive, and specific molecular diagnosis of pathogenic microorganisms in the eye. This technology has opened a new era in the diagnosis and treatment of uveitis, enabling physicians to establish new clinical entities of uveitis caused by infectious microorganisms, identify pathogens in the eyes of many patients with uveitis, and determine prompt diagnosis and appropriate therapy. Here we review the PCR process, new PCR tests specialized for ocular diseases, microorganisms detected by the PCR tests, diseases in the eye caused by these microorganisms, and the clinical characteristics, diagnosis, and therapy of uveitis.

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Baseline Factors Predictive of Visual Prognosis in Acute Postoperative Bacterial Endophthalmitis in Patients Undergoing Cataract Surgery

et al. 2013

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; for the French Institutional Endophthalmitis Study Group IMPORTANCE Although rare, postoperative endophthalmitis in patients undergoing cataract surgery can lead to anatomical or functional loss of the eye. Therapeutic strategies such as antibiotic prophylaxis and microbiological diagnosis are more effective with a target patient population. New prospective data are needed to identify prognostic factors. OBJECTIVE To identify baseline factors of visual prognosis in patients with acute bacterial endophthalmitis after cataract surgery. DESIGN Prospective study of consecutive patients undergoing cataract surgery, enrolled from March 1, 2004, through December 31, 2005. We analyzed outcomes to determine the effect on the final visual outcome, defined as poor (visual acuity [VA] worse than 20/100) or good (VA 20/40 or better) using univariate and multivariate analysis. SETTING Four academic hospitals. PARTICIPANTS Ninety-nine consecutive patients with cataract. INTERVENTION Corneal phacoemulsification. MAIN OUTCOMES AND MEASURES Factors related to the cataract surgery (complications), initial clinical presentation, and microbiological diagnosis and the final VA. RESULTS The significant baseline factors (at presentation) for good visual outcome (45% of the series) were the winter season, absence of complications during cataract surgery, initial VA, microbiological investigations revealing no microorganism or a coagulase-negative Staphylococcus species (CNSP), and fundus visibility. Quantitative factors associated with a good clinical prognosis were shorter duration of cataract surgery, younger age, and a hypopyon no greater than 1.5 mm. Significant factors associated with poor visual outcome were infection of the right eye, initial VA, corneal edema, a hypopyon larger than 1.5 mm, detection of bacterial species other than a CNSP, and the absence of fundus visibility. Multiple logistic regression analysis showed that high bacterial virulence was the only independent factor (odds ratio, 14.0 [95% CI, 2.7-71.0]; P = .001) for poor visual outcome. On the other hand, low bacterial virulence (odds ratio, 0.2 [95% CI, 0.03-0.6]; P = .01) and the absence of complications during cataract surgery (0.1 [0.01-0.4]; P = .003) were independent factors for good VA. CONCLUSIONS AND RELEVANCE Most clinical outcome factors in acute postoperative endophthalmitis can be identified at presentation. The bacterial virulence level is the main factor predictive of the final visual prognosis.

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Eubacterial PCR for Bacterial Detection and Identification in 100 Acute Postcataract Surgery Endophthalmitis

Cited by 116 publications

References 28 publications

Molecular biology applied to the laboratory diagnosis of bacterial endophthalmitis

Molecular biology applied to the laboratory diagnosis of bacterial endophthalmitis

A new era of uveitis: impact of polymerase chain reaction in intraocular inflammatory diseases

Baseline Factors Predictive of Visual Prognosis in Acute Postoperative Bacterial Endophthalmitis in Patients Undergoing Cataract Surgery

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